3H11
Catalog Fields
Antigen: laminin-1, avian
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Pittsburgh
Host Species: mouse
Depositors Notes: This antibody is avian specific. Use non-reducing conditions for western blotting.
Positive Tested Species Reactivity: Avian
Gene: LAMA1/LAMB1/LAMC1
Antigen Molecular Weight: Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Clonality: Monoclonal
Immunogen: Retinal basal laminae (E10)
Epitope Mapped: No
Myeloma Strain: X63Ag8.653
Immunogen Sequence: full length protein
Uniprot ID: F1NM47 Q01635 F1NI05
Additional Information: This antibody recognizes native embryonic chicken laminin.
Entrez Gene ID: 374016 396478 424442
Antibody Registry ID: AB_528342
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H11 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
14 References
Initial Publication
Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
Von Boxberg Y
The European journal of neuroscience 4.9 (1992): 840-852.