3H11

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$40.00
SKU: 3H11
View product citations for antibody 3H11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: laminin-1, avian
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Avian
Depositors Notes: This antibody is avian specific. Use non-reducing conditions for western blotting.
Antigen Molecular Weight: Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LAMA1/LAMB1/LAMC1
Immunogen: Retinal basal laminae (E10)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: F1NM47 Q01635 F1NI05 
Immunogen Sequence: full length protein
Entrez Gene ID: 374016 396478 424442 
Additional Characterization:
Antibody Registry ID: AB_528342 
Additional Information: This antibody recognizes native embryonic chicken laminin.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H11 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
    Von Boxberg Y
    The European journal of neuroscience 4.9 (1992): 840-852.

    A heparan sulfate proteoglycan in developing avian axonal tracts.
    Halfter W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.7 (1993 Jul): 2863-73.

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes and development 21.5 (2007 Mar 1): 531-6.

    Extracellular matrices of the avian ovarian follicle. Molecular characterization of chicken perlecan.
    Schneider WJ
    The Journal of biological chemistry 279.22 (2004 May 28): 23486-94.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    An ovomucin-like protein on the surface of migrating primordial germ cells of the chick and rat.
    Epperlein HH
    Development (Cambridge, England) 122.3 (1996 Mar): 915-23.

    Effects of Antitumor Drug Sorafenib on Chick Embryo Development.
    Yang XS
    Anatomical record (Hoboken, N.J. : 2007) 298.7 (2015 Jul): 1271-81.

    Hedgehog-BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos.
    Saito D
    Nature communications 7. (2016 Aug 25): 12561.

    In ovo gene manipulation of melanocytes and their adjacent keratinocytes during skin pigmentation of chicken embryos.
    Takahashi Y
    Development, growth and differentiation 57.3 (2015 Apr): 232-41.

    Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development.
    Caprile T
    Frontiers in neuroanatomy 10. (2016): 89.

    FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis.
    Takahashi Y
    Development (Cambridge, England) 142.13 (2015 Jul 1): 2329-37.

    Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet.
    Takahashi Y
    Proceedings of the National Academy of Sciences of the United States of America 111.18 (2014 May 6): 6660-5.

    An organizing function of basement membranes in the developing nervous system.
    Yip J
    Mechanisms of development 133. (2014 Aug): 1-10.

    WB References

    Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
    Von Boxberg Y
    The European journal of neuroscience 4.9 (1992): 840-852.

    A heparan sulfate proteoglycan in developing avian axonal tracts.
    Halfter W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.7 (1993 Jul): 2863-73.

    Biomechanical properties of native basement membranes.
    Lin H
    The FEBS journal 274.11 (2007 Jun): 2897-908.

    All References

    Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
    Von Boxberg Y
    The European journal of neuroscience 4.9 (1992): 840-852.

    A heparan sulfate proteoglycan in developing avian axonal tracts.
    Halfter W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.7 (1993 Jul): 2863-73.

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes and development 21.5 (2007 Mar 1): 531-6.

    Extracellular matrices of the avian ovarian follicle. Molecular characterization of chicken perlecan.
    Schneider WJ
    The Journal of biological chemistry 279.22 (2004 May 28): 23486-94.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    An ovomucin-like protein on the surface of migrating primordial germ cells of the chick and rat.
    Epperlein HH
    Development (Cambridge, England) 122.3 (1996 Mar): 915-23.

    Effects of Antitumor Drug Sorafenib on Chick Embryo Development.
    Yang XS
    Anatomical record (Hoboken, N.J. : 2007) 298.7 (2015 Jul): 1271-81.

    Hedgehog-BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos.
    Saito D
    Nature communications 7. (2016 Aug 25): 12561.

    In ovo gene manipulation of melanocytes and their adjacent keratinocytes during skin pigmentation of chicken embryos.
    Takahashi Y
    Development, growth and differentiation 57.3 (2015 Apr): 232-41.

    Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development.
    Caprile T
    Frontiers in neuroanatomy 10. (2016): 89.

    FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis.
    Takahashi Y
    Development (Cambridge, England) 142.13 (2015 Jul 1): 2329-37.

    Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet.
    Takahashi Y
    Proceedings of the National Academy of Sciences of the United States of America 111.18 (2014 May 6): 6660-5.

    An organizing function of basement membranes in the developing nervous system.
    Yip J
    Mechanisms of development 133. (2014 Aug): 1-10.

    Biomechanical properties of native basement membranes.
    Lin H
    The FEBS journal 274.11 (2007 Jun): 2897-908.

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