3H11
Catalog Fields
Antigen: laminin-1, avian
Available to For-Profits: Yes
Antigen Species: chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Halfter, W.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian
Depositors Institution: University of Pittsburgh
Antigen Molecular Weight: Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Depositors Notes: This antibody is avian specific. Use non-reducing conditions for western blotting.
Immunogen: Retinal basal laminae (E10)
Gene: LAMA1/LAMB1/LAMC1
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: X63Ag8.653
Epitope Mapped: No
Uniprot ID: F1NM47 Q01635 F1NI05
Epitope Location or Sequence:
Entrez Gene ID: 374016 396478 424442
Immunogen Sequence: full length protein
Antibody Registry ID: AB_528342
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: This antibody recognizes native embryonic chicken laminin.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H11 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
Von Boxberg Y
The European journal of neuroscience 4.9 (1992): 840-852.