3H11
Catalog Fields
Antigen: laminin-1, avian
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Avian
Depositors Notes: This antibody is avian specific. Use non-reducing conditions for western blotting.
Antigen Molecular Weight: Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Predicted Species Reactivity:
Gene: LAMA1/LAMB1/LAMC1
Immunogen: Retinal basal laminae (E10)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: F1NM47 Q01635 F1NI05
Immunogen Sequence: full length protein
Entrez Gene ID: 374016 396478 424442
Antibody Registry ID: AB_528342
Additional Information: This antibody recognizes native embryonic chicken laminin.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H11 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
Von Boxberg Y
The European journal of neuroscience 4.9 (1992): 840-852.