3H11

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$45.00 to $425.00
SKU: 3H11
View product citations for antibody 3H11 on CiteAb

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Available: 60

DSHB Data Sheet

Catalog Fields

Antigen: laminin-1, avian
Available to For-Profits: Yes
Antigen Species: chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Halfter, W.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian
Depositors Institution: University of Pittsburgh
Antigen Molecular Weight: Apparent: 200 kDa (beta1 and gamma1) and 400 kDa (alpha1)
Depositors Notes: This antibody is avian specific. Use non-reducing conditions for western blotting.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Retinal basal laminae (E10)
Gene: LAMA1/LAMB1/LAMC1
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: X63Ag8.653
Epitope Mapped: No
Uniprot ID: F1NM47 Q01635 F1NI05 
Epitope Location or Sequence:
Entrez Gene ID: 374016 396478 424442 
Immunogen Sequence: full length protein
Antibody Registry ID: AB_528342 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: This antibody recognizes native embryonic chicken laminin.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H11 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
    Von Boxberg Y
    The European journal of neuroscience 4.9 (1992): 840-852.

    A heparan sulfate proteoglycan in developing avian axonal tracts.
    Halfter W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.7 (1993 Jul): 2863-73.

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes & development 21.5 (2007 Mar 1): 531-6.

    Extracellular matrices of the avian ovarian follicle. Molecular characterization of chicken perlecan.
    Schneider WJ
    The Journal of biological chemistry 279.22 (2004 May 28): 23486-94.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    An ovomucin-like protein on the surface of migrating primordial germ cells of the chick and rat.
    Epperlein HH
    Development (Cambridge, England) 122.3 (1996 Mar): 915-23.

    Effects of Antitumor Drug Sorafenib on Chick Embryo Development.
    Yang XS
    Anatomical record (Hoboken, N.J. : 2007) 298.7 (2015 Jul): 1271-81.

    Hedgehog-BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos.
    Saito D
    Nature communications 7. (2016 Aug 25): 12561.

    In ovo gene manipulation of melanocytes and their adjacent keratinocytes during skin pigmentation of chicken embryos.
    Takahashi Y
    Development, growth & differentiation 57.3 (2015 Apr): 232-41.

    Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development.
    Caprile T
    Frontiers in neuroanatomy 10. (2016): 89.

    FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis.
    Takahashi Y
    Development (Cambridge, England) 142.13 (2015 Jul 1): 2329-37.

    Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet.
    Takahashi Y
    Proceedings of the National Academy of Sciences of the United States of America 111.18 (2014 May 6): 6660-5.

    An organizing function of basement membranes in the developing nervous system.
    Yip J
    Mechanisms of development 133. (2014 Aug): 1-10.

    Neurospheres obtained from the ciliary margin of the chicken eye possess positional values and retinal ganglion cells differentiated from them respond to EphA/ephrin-A system.
    Scicolone G
    Experimental eye research 217. (2022 Apr): 108965.

    WB References

    Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
    Von Boxberg Y
    The European journal of neuroscience 4.9 (1992): 840-852.

    A heparan sulfate proteoglycan in developing avian axonal tracts.
    Halfter W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.7 (1993 Jul): 2863-73.

    Biomechanical properties of native basement membranes.
    Lin H
    The FEBS journal 274.11 (2007 Jun): 2897-908.

    IHC References

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    All References

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    Axonal Growth on Solubilized and Reconstituted Matrix from the Embryonic Chicken Retina Inner Limiting Membrane.
    Von Boxberg Y
    The European journal of neuroscience 4.9 (1992): 840-852.

    A heparan sulfate proteoglycan in developing avian axonal tracts.
    Halfter W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.7 (1993 Jul): 2863-73.

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes & development 21.5 (2007 Mar 1): 531-6.

    Extracellular matrices of the avian ovarian follicle. Molecular characterization of chicken perlecan.
    Schneider WJ
    The Journal of biological chemistry 279.22 (2004 May 28): 23486-94.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    An ovomucin-like protein on the surface of migrating primordial germ cells of the chick and rat.
    Epperlein HH
    Development (Cambridge, England) 122.3 (1996 Mar): 915-23.

    Effects of Antitumor Drug Sorafenib on Chick Embryo Development.
    Yang XS
    Anatomical record (Hoboken, N.J. : 2007) 298.7 (2015 Jul): 1271-81.

    Hedgehog-BMP signalling establishes dorsoventral patterning in lateral plate mesoderm to trigger gonadogenesis in chicken embryos.
    Saito D
    Nature communications 7. (2016 Aug 25): 12561.

    In ovo gene manipulation of melanocytes and their adjacent keratinocytes during skin pigmentation of chicken embryos.
    Takahashi Y
    Development, growth & differentiation 57.3 (2015 Apr): 232-41.

    Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development.
    Caprile T
    Frontiers in neuroanatomy 10. (2016): 89.

    FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis.
    Takahashi Y
    Development (Cambridge, England) 142.13 (2015 Jul 1): 2329-37.

    Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet.
    Takahashi Y
    Proceedings of the National Academy of Sciences of the United States of America 111.18 (2014 May 6): 6660-5.

    An organizing function of basement membranes in the developing nervous system.
    Yip J
    Mechanisms of development 133. (2014 Aug): 1-10.

    Neurospheres obtained from the ciliary margin of the chicken eye possess positional values and retinal ganglion cells differentiated from them respond to EphA/ephrin-A system.
    Scicolone G
    Experimental eye research 217. (2022 Apr): 108965.

    Biomechanical properties of native basement membranes.
    Lin H
    The FEBS journal 274.11 (2007 Jun): 2897-908.

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