VN 3-24
Catalog Fields
Clone ID/Product Name: VN 3-24
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: VCL
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2214518
Uniprot ID: P12003
Entrez Gene ID: 396422
Clonality: Monoclonal
Immunogen: Purified vinculin from gizzards
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: Vinculin/meta-vinculin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/22/1988
Antigen Molecular Weight: 130kDa vinculin; 150 kDa meta-vinculin
Depositor: Saga, S.
Antigen Sequence:
Depositor Institution: Nagoya University
Antigen Species: Chick
Depositor Notes: Meta-vinculin is expressed exclusively in muscular tissues. This antibody does not react with human, mouse and rat vinculin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Xenopus
Additional Information: This antibody does not react with human, mouse or rat. RRID: AB_2214518
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VN 3-24 was deposited to the DSHB by Saga, S. (DSHB Hybridoma Product VN 3-24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
Kojima K
Experimental cell research 156.1 (1985 Jan): 45-56.