VN 3-24

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$45.00 to $425.00
SKU: VN 3-24
View product citations for antibody VN 3-24 on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Antigen: Vinculin/meta-vinculin
Available to For-Profits: Yes
Antigen Species: Chick
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Saga, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Xenopus
Depositors Institution: Nagoya University
Antigen Molecular Weight: 130kDa vinculin; 150 kDa meta-vinculin
Depositors Notes: Meta-vinculin is expressed exclusively in muscular tissues. This antibody does not react with human, mouse and rat vinculin.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Purified vinculin from gizzards
Gene: VCL
Alternate Antibody Name:
Alternate Gene Names: VINC1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: No
Uniprot ID: P12003 
Epitope Location or Sequence:
Entrez Gene ID: 396422 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2214518 
Additional Characterization:  
Recommended Applications: Western Blot
Additional Information: This antibody does not react with human, mouse or rat. RRID: AB_2214518
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VN 3-24 was deposited to the DSHB by Saga, S. (DSHB Hybridoma Product VN 3-24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
    Kojima K
    Experimental cell research 156.1 (1985 Jan): 45-56.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Subcellular localization of dystrophin and vinculin in cardiac muscle fibers and fibers of the conduction system of the chicken ventricle.
    Shimada Y
    Cell and tissue research 294.1 (1998 Oct): 137-43.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    The chicken cornea as a model of wound healing and neuronal re-innervation.
    Fischer AJ
    Molecular vision 17. (2011): 2440-54.

    Enabled (Xena) regulates neural plate morphogenesis, apical constriction, and cellular adhesion required for neural tube closure in Xenopus.
    Miller JR
    Developmental biology 314.2 (2008 Feb 15): 393-403.

    Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development.
    Miller JR
    Development (Cambridge, England) 133.4 (2006 Feb): 685-95.

    Subcellular localization of dystrophin and vinculin in cardiac muscle fibers and fibers of the conduction system of the chicken ventricle.
    Shimada Y
    Cell and tissue research 294.1 (1998 Oct): 137-43.

    Protein kinase C regulates the recruitment of syndecan-4 into focal contacts.
    Goetinck PF
    Molecular biology of the cell 6.11 (1995 Nov): 1503-13.

    Rearrangement of mRNAs for costamere proteins during costamere development in cultured skeletal muscle from chicken.
    Fulton AB
    Journal of cell science 107 ( Pt 3). (1994 Mar): 377-86.

    WB References
    IHC References

    Microfilament-membrane interactions in Xenopus myocytes.
    Bloch RJ
    Cell motility and the cytoskeleton 35.1 (1996): 68-80.

    ELISA References
    All References

    Microfilament-membrane interactions in Xenopus myocytes.
    Bloch RJ
    Cell motility and the cytoskeleton 35.1 (1996): 68-80.

    Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
    Kojima K
    Experimental cell research 156.1 (1985 Jan): 45-56.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Subcellular localization of dystrophin and vinculin in cardiac muscle fibers and fibers of the conduction system of the chicken ventricle.
    Shimada Y
    Cell and tissue research 294.1 (1998 Oct): 137-43.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    The chicken cornea as a model of wound healing and neuronal re-innervation.
    Fischer AJ
    Molecular vision 17. (2011): 2440-54.

    Enabled (Xena) regulates neural plate morphogenesis, apical constriction, and cellular adhesion required for neural tube closure in Xenopus.
    Miller JR
    Developmental biology 314.2 (2008 Feb 15): 393-403.

    Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development.
    Miller JR
    Development (Cambridge, England) 133.4 (2006 Feb): 685-95.

    Protein kinase C regulates the recruitment of syndecan-4 into focal contacts.
    Goetinck PF
    Molecular biology of the cell 6.11 (1995 Nov): 1503-13.

    Rearrangement of mRNAs for costamere proteins during costamere development in cultured skeletal muscle from chicken.
    Fulton AB
    Journal of cell science 107 ( Pt 3). (1994 Mar): 377-86.

    Structures linking microfilament bundles to the membrane at focal contacts.
    Bloch RJ
    The Journal of cell biology 122.2 (1993 Jul): 485-96.

    v-Src inhibits myogenic differentiation by interfering with the regulatory network of muscle-specific transcriptional activators at multiple levels.
    Alemà S
    Oncogene 22.51 (2003 Nov 13): 8302-15.

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