VN 3-24

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$40.00
SKU: VN 3-24
View product citations for antibody VN 3-24 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Vinculin/meta-vinculin
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: Saga, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Nagoya University
Positive Tested Species Reactivity: Chicken, Xenopus
Depositors Notes: Meta-vinculin is expressed exclusively in muscular tissues. This antibody does not react with human, mouse and rat vinculin.
Antigen Molecular Weight: 130kDa vinculin; 150 kDa meta-vinculin
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: VCL
Immunogen: Purified vinculin from gizzards
Alternate Gene Names: VINC1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P12003 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396422 
Additional Characterization:
Antibody Registry ID: AB_2214518 
Additional Information: This antibody does not react with human, mouse or rat. RRID: AB_2214518
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VN 3-24 was deposited to the DSHB by Saga, S. (DSHB Hybridoma Product VN 3-24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
    Kojima K
    Experimental cell research 156.1 (1985 Jan): 45-56.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Subcellular localization of dystrophin and vinculin in cardiac muscle fibers and fibers of the conduction system of the chicken ventricle.
    Shimada Y
    Cell and tissue research 294.1 (1998 Oct): 137-43.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    The chicken cornea as a model of wound healing and neuronal re-innervation.
    Fischer AJ
    Molecular vision 17. (2011): 2440-54.

    Enabled (Xena) regulates neural plate morphogenesis, apical constriction, and cellular adhesion required for neural tube closure in Xenopus.
    Miller JR
    Developmental biology 314.2 (2008 Feb 15): 393-403.

    Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development.
    Miller JR
    Development (Cambridge, England) 133.4 (2006 Feb): 685-95.

    Subcellular localization of dystrophin and vinculin in cardiac muscle fibers and fibers of the conduction system of the chicken ventricle.
    Shimada Y
    Cell and tissue research 294.1 (1998 Oct): 137-43.

    Protein kinase C regulates the recruitment of syndecan-4 into focal contacts.
    Goetinck PF
    Molecular biology of the cell 6.11 (1995 Nov): 1503-13.

    Rearrangement of mRNAs for costamere proteins during costamere development in cultured skeletal muscle from chicken.
    Fulton AB
    Journal of cell science 107 ( Pt 3). (1994 Mar): 377-86.

    WB References
    IHC References

    Microfilament-membrane interactions in Xenopus myocytes.
    Bloch RJ
    Cell motility and the cytoskeleton 35.1 (1996): 68-80.

    ELISA References
    All References

    Microfilament-membrane interactions in Xenopus myocytes.
    Bloch RJ
    Cell motility and the cytoskeleton 35.1 (1996): 68-80.

    Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
    Kojima K
    Experimental cell research 156.1 (1985 Jan): 45-56.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Subcellular localization of dystrophin and vinculin in cardiac muscle fibers and fibers of the conduction system of the chicken ventricle.
    Shimada Y
    Cell and tissue research 294.1 (1998 Oct): 137-43.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    The chicken cornea as a model of wound healing and neuronal re-innervation.
    Fischer AJ
    Molecular vision 17. (2011): 2440-54.

    Enabled (Xena) regulates neural plate morphogenesis, apical constriction, and cellular adhesion required for neural tube closure in Xenopus.
    Miller JR
    Developmental biology 314.2 (2008 Feb 15): 393-403.

    Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development.
    Miller JR
    Development (Cambridge, England) 133.4 (2006 Feb): 685-95.

    Protein kinase C regulates the recruitment of syndecan-4 into focal contacts.
    Goetinck PF
    Molecular biology of the cell 6.11 (1995 Nov): 1503-13.

    Rearrangement of mRNAs for costamere proteins during costamere development in cultured skeletal muscle from chicken.
    Fulton AB
    Journal of cell science 107 ( Pt 3). (1994 Mar): 377-86.

    Structures linking microfilament bundles to the membrane at focal contacts.
    Bloch RJ
    The Journal of cell biology 122.2 (1993 Jul): 485-96.

    v-Src inhibits myogenic differentiation by interfering with the regulatory network of muscle-specific transcriptional activators at multiple levels.
    Alemà S
    Oncogene 22.51 (2003 Nov 13): 8302-15.

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