znt-1

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$40.00
SKU: znt-1-s
View product citations for antibody znt-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: notochord and neuropil marker
Hybridoma Cells Available: No
Antigen Species: zebrafish
Depositor: Trevarrow, B.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Neuroscience, University of Oregon
Positive Tested Species Reactivity: Bony fish, Tunicate, Zebrafish
Depositors Notes: Doesn't work on Xenopus, chicken, shark, medaka or trout. Works on zebrafish embryos/larva and adults, betta fish, Haplochromis burtoni and tunicates. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat) the cell surface of notochord, actinotrichia, neuropil, pectoral fin tip and otocyst.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Adult brain membranes. Boosts: adult CNS membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531911 
Additional Information:
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
znt-1 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product znt-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis.
    Cheng SH
    Toxicological sciences : an official journal of the Society of Toxicology 73.1 (2003 May): 149-59.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis.
    Cheng SH
    Toxicological sciences : an official journal of the Society of Toxicology 73.1 (2003 May): 149-59.

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