ZN-8

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$40.00
SKU: ZN-8
View product citations for antibody ZN-8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD166 antigen homolog A (Neurolin)
Hybridoma Cells Available: No
Antigen Species: zebrafish
Depositor: Trevarrow, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Neuroscience, University of Oregon
Positive Tested Species Reactivity: Zebrafish
Depositors Notes: Doesn't work on Xenopus (st36), betta fish (1d, 2d), chicken, shark and Haplochromis burtoni. Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface on commissural fibers, secondary Motoneurons, outer surface of somites, some ganglia, radial glial cells and the anterior commissure.
Antigen Molecular Weight: Predicted: 59 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: alcama
Immunogen: Zebrafish embryo membranes (2 days old)
Alternate Gene Names: alcam, cd166, DM-GRASP
Alternate Antibody Name:
Clonality:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: Q90460 
Immunogen Sequence: Full length protein
Entrez Gene ID: 30194 
Additional Characterization:  
Antibody Registry ID: AB_531904 
Additional Information: zn-5 and zn-8 are two clones from the same hybridoma. Recognizes secondary motor neurones.
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ZN-8 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product ZN-8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References

    The homeobox gene mbx is involved in eye and tectum development.
    Dawid IB
    Developmental biology 248.1 (2002 Aug 1): 107-17.

    Xirp proteins mark injured skeletal muscle in zebrafish.
    Abdelilah-Seyfried S
    PloS one 7.2 (2012): e31041.

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Tcf7l1 is required for spinal cord progenitor maintenance.
    Dorsky RI
    Developmental dynamics : an official publication of the American Association of Anatomists 240.10 (2011 Oct): 2256-64.

    Vgll2a is required for neural crest cell survival during zebrafish craniofacial development.
    Artinger KB
    Developmental biology 357.1 (2011 Sep 1): 269-81.

    Apc1-mediated antagonism of Wnt/beta-catenin signaling is required for retino-tectal pathfinding in the zebrafish.
    Zivkovic D
    Zebrafish 6.1 (2009 Mar): 41-7.

    Analysis of the retina in the zebrafish model.
    Avanesov A
    Methods in cell biology 134. (2016): 257-334.

    The Roles of RNA Polymerase I and III Subunits Polr1c and Polr1d in Craniofacial Development and in Zebrafish Models of Treacher Collins Syndrome.
    Trainor PA
    PLoS genetics 12.7 (2016 Jul): e1006187.

    Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish.
    Ali DW
    Neurotoxicology and teratology 48. (2015 Mar-Apr): 18-27.

    Effects of BDE-209 contaminated sediments on zebrafish development and potential implications to human health.
    Raldúa D
    Environment international 63. (2014 Feb): 216-23.

    The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes.
    Chen JN
    eLife 6. (2017 Aug 19): .

    Sox2 expression in the visual system of two teleost species.
    Mack AF
    Brain research 1722. (2019 Nov 1): 146350.

    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division.
    Kane DA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 828-43.

    Autotaxin/ENPP2 regulates oligodendrocyte differentiation in vivo in the developing zebrafish hindbrain.
    Fuss B
    Glia 60.10 (2012 Oct): 1605-18.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division.
    Kane DA
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 828-43.

    Autotaxin/ENPP2 regulates oligodendrocyte differentiation in vivo in the developing zebrafish hindbrain.
    Fuss B
    Glia 60.10 (2012 Oct): 1605-18.

    The homeobox gene mbx is involved in eye and tectum development.
    Dawid IB
    Developmental biology 248.1 (2002 Aug 1): 107-17.

    Xirp proteins mark injured skeletal muscle in zebrafish.
    Abdelilah-Seyfried S
    PloS one 7.2 (2012): e31041.

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Tcf7l1 is required for spinal cord progenitor maintenance.
    Dorsky RI
    Developmental dynamics : an official publication of the American Association of Anatomists 240.10 (2011 Oct): 2256-64.

    Vgll2a is required for neural crest cell survival during zebrafish craniofacial development.
    Artinger KB
    Developmental biology 357.1 (2011 Sep 1): 269-81.

    Apc1-mediated antagonism of Wnt/beta-catenin signaling is required for retino-tectal pathfinding in the zebrafish.
    Zivkovic D
    Zebrafish 6.1 (2009 Mar): 41-7.

    Analysis of the retina in the zebrafish model.
    Avanesov A
    Methods in cell biology 134. (2016): 257-334.

    The Roles of RNA Polymerase I and III Subunits Polr1c and Polr1d in Craniofacial Development and in Zebrafish Models of Treacher Collins Syndrome.
    Trainor PA
    PLoS genetics 12.7 (2016 Jul): e1006187.

    Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish.
    Ali DW
    Neurotoxicology and teratology 48. (2015 Mar-Apr): 18-27.

    Effects of BDE-209 contaminated sediments on zebrafish development and potential implications to human health.
    Raldúa D
    Environment international 63. (2014 Feb): 216-23.

    The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes.
    Chen JN
    eLife 6. (2017 Aug 19): .

    Sox2 expression in the visual system of two teleost species.
    Mack AF
    Brain research 1722. (2019 Nov 1): 146350.

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