F59

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$40.00
SKU: F59
View product citations for antibody F59 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (all fast isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University Medical Center
Positive Tested Species Reactivity: Amphibian, Avian, Canine, Fish, Human, Mouse, Quail, Rabbit, Rat, Shark, Turtle, Xenopus, Zebrafish
Depositors Notes: This antibody is specific for myosin heavy chain in fast contracting skeletal muscle and atrial and ventricular myosin heavy chains.
Antigen Molecular Weight: ~223 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH1A
Immunogen: Myosin isolated from pectoralis major
Alternate Gene Names: MYH1, MYH3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Location or Sequence: a.a. 200-250
Uniprot ID: Q91352 
Immunogen Sequence: Full length protein
Entrez Gene ID: 417309 
Additional Characterization:
Antibody Registry ID: AB_528373 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F59 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F59)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

38 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IF References

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    CKIP-1 regulates mammalian and zebrafish myoblast fusion.
    Goillot E
    Journal of cell science 125.Pt 16 (2012 Aug 15): 3790-800.

    Immortalized myogenic cells from congenital muscular dystrophy type1A patients recapitulate aberrant caspase activation in pathogenesis: a new tool for MDC1A research.
    Miller JB
    Skeletal muscle 3.1 (2013 Dec 6): 28.

    Scube activity is necessary for Hedgehog signal transduction in vivo.
    Currie PD
    Developmental biology 368.2 (2012 Aug 15): 193-202.

    Smyd1b is required for skeletal and cardiac muscle function in zebrafish.
    Du SJ
    Molecular biology of the cell 24.22 (2013 Nov): 3511-21.

    A novel approach to study motor neurons from zebrafish embryos and larvae in culture.
    Feldman EL
    Journal of neuroscience methods 205.2 (2012 Apr 15): 277-82.

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Xirp proteins mark injured skeletal muscle in zebrafish.
    Abdelilah-Seyfried S
    PloS one 7.2 (2012): e31041.

    The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity.
    Lecker SH
    The Journal of clinical investigation 117.12 (2007 Dec): 3940-51.

    Fibronectin is deposited by injury-activated epicardial cells and is necessary for zebrafish heart regeneration.
    Poss KD
    Developmental biology 382.2 (2013 Oct 15): 427-35.

    Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene.
    Jones TI
    Molecular and cellular biology 34.11 (2014 Jun): 1942-55.

    Restricted expression of cdc25a in the tailbud is essential for formation of the zebrafish posterior body.
    Kimelman D
    Genes and development 28.4 (2014 Feb 15): 384-95.

    In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration.
    Poss KD
    Development (Cambridge, England) 140.3 (2013 Feb 1): 660-6.

    Macondo crude oil from the Deepwater Horizon oil spill disrupts specific developmental processes during zebrafish embryogenesis.
    Barresi MJ
    BMC biology 10. (2012 May 4): 40.

    Divergence of zebrafish and mouse lymphatic cell fate specification pathways.
    Schulte-Merker S
    Development (Cambridge, England) 141.6 (2014 Mar): 1228-38.

    [Pathogenetic and legal insurance problems in the demonstration of atypical mycobacteria as cause of occupational diseases].
    Bassermann FJ
    Praxis der Pneumologie 26.12 (1972 Dec): 677-87.

    The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction.
    Henry CA
    Skeletal muscle 6. (2016): 18.

    Acetylcholine and calcium signalling regulates muscle fibre formation in the zebrafish embryo.
    Ashworth R
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5181-90.

    WB References

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Evolutionarily conserved sequences of striated muscle myosin heavy chain isoforms. Epitope mapping by cDNA expression.
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

    The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation.
    Pan H
    The FEBS journal 281.13 (2014 Jul): 2990-3003.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Mutations in KLHL40 are a frequent cause of severe autosomal-recessive nemaline myopathy.
    Laing NG
    American journal of human genetics 93.1 (2013 Jul 11): 6-18.

    IHC References

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Overdose of D-serine Induces Movement Disorder and Neuromuscular Changes of Zebrafish Larvae.
    Chen YH
    Journal of toxicologic pathology 27.1 (2014 Apr): 19-24.

    Toxicity assessments of chalcone and some synthetic chalcone analogues in a zebrafish model.
    Chen YH
    Molecules (Basel, Switzerland) 19.1 (2014 Jan 7): 641-50.

    SCUBE3 (signal peptide-CUB-EGF domain-containing protein 3) modulates fibroblast growth factor signaling during fast muscle development.
    Yang RB
    The Journal of biological chemistry 289.27 (2014 Jul 4): 18928-42.

    The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation.
    Pan H
    The FEBS journal 281.13 (2014 Jul): 2990-3003.

    Short-term exposure of zebrafish embryos to arecoline leads to retarded growth, motor impairment, and somite muscle fiber changes.
    Kung HN
    Zebrafish 12.1 (2015 Feb): 58-70.

    Heat-shock-mediated conditional regulation of hedgehog/gli signaling in zebrafish.
    Karlstrom R
    Developmental dynamics : an official publication of the American Association of Anatomists 242.5 (2013 May): 539-49.

    Lampreys have a single gene cluster for the fast skeletal myosin heavy chain gene family.
    Watabe S
    PloS one 8.12 (2013): e85500.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Vincristine and bortezomib cause axon outgrowth and behavioral defects in larval zebrafish.
    Hutson LD
    Journal of the peripheral nervous system : JPNS 17.1 (2012 Mar): 76-89.

    Expression of DUX4 in zebrafish development recapitulates facioscapulohumeral muscular dystrophy.
    Kunkel LM
    Human molecular genetics 22.3 (2013 Feb 1): 568-77.

    Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment.
    Chen YH
    Journal of applied toxicology : JAT 35.3 (2015 Mar): 287-94.

    Mutations in KLHL40 are a frequent cause of severe autosomal-recessive nemaline myopathy.
    Laing NG
    American journal of human genetics 93.1 (2013 Jul 11): 6-18.

    Intestinal mucosa in nephropathic cystinosis.
    Shiloh H
    Journal of pediatric gastroenterology and nutrition 6.3 (1987 May-Jun): 359-64.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Myosin isozyme expression in response to stretch-induced hypertrophy in the Japanese quail.
    Gonyea WJ
    The Anatomical record 228.3 (1990 Nov): 255-61.

    The appearance of acetylated alpha-tubulin during early development and cellular differentiation in Xenopus.
    Klymkowsky MW
    Developmental biology 136.1 (1989 Nov): 104-17.

    Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish.
    Mercader N
    Cell reports 28.5 (2019 Jul 30): 1296-1306.e6.

    IP References

    Evolutionarily conserved sequences of striated muscle myosin heavy chain isoforms. Epitope mapping by cDNA expression.
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

    Epitope Map References

    Evolutionarily conserved sequences of striated muscle myosin heavy chain isoforms. Epitope mapping by cDNA expression.
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

    All References

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Overdose of D-serine Induces Movement Disorder and Neuromuscular Changes of Zebrafish Larvae.
    Chen YH
    Journal of toxicologic pathology 27.1 (2014 Apr): 19-24.

    Toxicity assessments of chalcone and some synthetic chalcone analogues in a zebrafish model.
    Chen YH
    Molecules (Basel, Switzerland) 19.1 (2014 Jan 7): 641-50.

    SCUBE3 (signal peptide-CUB-EGF domain-containing protein 3) modulates fibroblast growth factor signaling during fast muscle development.
    Yang RB
    The Journal of biological chemistry 289.27 (2014 Jul 4): 18928-42.

    The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation.
    Pan H
    The FEBS journal 281.13 (2014 Jul): 2990-3003.

    Short-term exposure of zebrafish embryos to arecoline leads to retarded growth, motor impairment, and somite muscle fiber changes.
    Kung HN
    Zebrafish 12.1 (2015 Feb): 58-70.

    Heat-shock-mediated conditional regulation of hedgehog/gli signaling in zebrafish.
    Karlstrom R
    Developmental dynamics : an official publication of the American Association of Anatomists 242.5 (2013 May): 539-49.

    Lampreys have a single gene cluster for the fast skeletal myosin heavy chain gene family.
    Watabe S
    PloS one 8.12 (2013): e85500.

    Muscle development and differentiation in the urodele Ambystoma mexicanum.
    Grimaldi A
    Development, growth and differentiation 54.4 (2012 May): 489-502.

    Vincristine and bortezomib cause axon outgrowth and behavioral defects in larval zebrafish.
    Hutson LD
    Journal of the peripheral nervous system : JPNS 17.1 (2012 Mar): 76-89.

    Expression of DUX4 in zebrafish development recapitulates facioscapulohumeral muscular dystrophy.
    Kunkel LM
    Human molecular genetics 22.3 (2013 Feb 1): 568-77.

    Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment.
    Chen YH
    Journal of applied toxicology : JAT 35.3 (2015 Mar): 287-94.

    Mutations in KLHL40 are a frequent cause of severe autosomal-recessive nemaline myopathy.
    Laing NG
    American journal of human genetics 93.1 (2013 Jul 11): 6-18.

    Intestinal mucosa in nephropathic cystinosis.
    Shiloh H
    Journal of pediatric gastroenterology and nutrition 6.3 (1987 May-Jun): 359-64.

    Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    Myosin isozyme expression in response to stretch-induced hypertrophy in the Japanese quail.
    Gonyea WJ
    The Anatomical record 228.3 (1990 Nov): 255-61.

    The appearance of acetylated alpha-tubulin during early development and cellular differentiation in Xenopus.
    Klymkowsky MW
    Developmental biology 136.1 (1989 Nov): 104-17.

    Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish.
    Mercader N
    Cell reports 28.5 (2019 Jul 30): 1296-1306.e6.

    CKIP-1 regulates mammalian and zebrafish myoblast fusion.
    Goillot E
    Journal of cell science 125.Pt 16 (2012 Aug 15): 3790-800.

    Immortalized myogenic cells from congenital muscular dystrophy type1A patients recapitulate aberrant caspase activation in pathogenesis: a new tool for MDC1A research.
    Miller JB
    Skeletal muscle 3.1 (2013 Dec 6): 28.

    Scube activity is necessary for Hedgehog signal transduction in vivo.
    Currie PD
    Developmental biology 368.2 (2012 Aug 15): 193-202.

    Smyd1b is required for skeletal and cardiac muscle function in zebrafish.
    Du SJ
    Molecular biology of the cell 24.22 (2013 Nov): 3511-21.

    A novel approach to study motor neurons from zebrafish embryos and larvae in culture.
    Feldman EL
    Journal of neuroscience methods 205.2 (2012 Apr 15): 277-82.

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Xirp proteins mark injured skeletal muscle in zebrafish.
    Abdelilah-Seyfried S
    PloS one 7.2 (2012): e31041.

    The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity.
    Lecker SH
    The Journal of clinical investigation 117.12 (2007 Dec): 3940-51.

    Fibronectin is deposited by injury-activated epicardial cells and is necessary for zebrafish heart regeneration.
    Poss KD
    Developmental biology 382.2 (2013 Oct 15): 427-35.

    Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene.
    Jones TI
    Molecular and cellular biology 34.11 (2014 Jun): 1942-55.

    Restricted expression of cdc25a in the tailbud is essential for formation of the zebrafish posterior body.
    Kimelman D
    Genes and development 28.4 (2014 Feb 15): 384-95.

    In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration.
    Poss KD
    Development (Cambridge, England) 140.3 (2013 Feb 1): 660-6.

    Macondo crude oil from the Deepwater Horizon oil spill disrupts specific developmental processes during zebrafish embryogenesis.
    Barresi MJ
    BMC biology 10. (2012 May 4): 40.

    Divergence of zebrafish and mouse lymphatic cell fate specification pathways.
    Schulte-Merker S
    Development (Cambridge, England) 141.6 (2014 Mar): 1228-38.

    [Pathogenetic and legal insurance problems in the demonstration of atypical mycobacteria as cause of occupational diseases].
    Bassermann FJ
    Praxis der Pneumologie 26.12 (1972 Dec): 677-87.

    The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction.
    Henry CA
    Skeletal muscle 6. (2016): 18.

    Acetylcholine and calcium signalling regulates muscle fibre formation in the zebrafish embryo.
    Ashworth R
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5181-90.

    Evolutionarily conserved sequences of striated muscle myosin heavy chain isoforms. Epitope mapping by cDNA expression.
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

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