F59

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$40.00
SKU: F59
View product citations for antibody F59 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (all fast isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University Medical Center
Positive Tested Species Reactivity: Amphibian, Avian, Canine, Fish, Human, Mouse, Quail, Rabbit, Rat, Shark, Turtle, Xenopus, Zebrafish
Depositors Notes: This antibody is specific for myosin heavy chain in fast contracting skeletal muscle and atrial and ventricular myosin heavy chains.
Antigen Molecular Weight: ~223 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH1A
Immunogen: Myosin isolated from pectoralis major
Alternate Gene Names: MYH1, MYH3
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Location or Sequence: a.a. 200-250
Uniprot ID: Q91352 
Immunogen Sequence: Full length protein
Entrez Gene ID: 417309 
Additional Characterization:
Antibody Registry ID: AB_528373 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F59 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F59)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

38 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of cell biology
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IF References

    The Journal of cell biology
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Journal of cell science
    Goillot E
    Journal of cell science 125.Pt 16 (2012 Aug 15): 3790-800.

    Skeletal muscle
    Miller JB
    Skeletal muscle 3.1 (2013 Dec 6): 28.

    Developmental biology
    Currie PD
    Developmental biology 368.2 (2012 Aug 15): 193-202.

    Molecular biology of the cell
    Du SJ
    Molecular biology of the cell 24.22 (2013 Nov): 3511-21.

    Journal of neuroscience methods
    Feldman EL
    Journal of neuroscience methods 205.2 (2012 Apr 15): 277-82.

    Developmental biology
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    PloS one
    Abdelilah-Seyfried S
    PloS one 7.2 (2012): e31041.

    The Journal of clinical investigation
    Lecker SH
    The Journal of clinical investigation 117.12 (2007 Dec): 3940-51.

    Developmental biology
    Poss KD
    Developmental biology 382.2 (2013 Oct 15): 427-35.

    Molecular and cellular biology
    Jones TI
    Molecular and cellular biology 34.11 (2014 Jun): 1942-55.

    Genes & development
    Kimelman D
    Genes & development 28.4 (2014 Feb 15): 384-95.

    Development (Cambridge, England)
    Poss KD
    Development (Cambridge, England) 140.3 (2013 Feb 1): 660-6.

    BMC biology
    Barresi MJ
    BMC biology 10. (2012 May 4): 40.

    Development (Cambridge, England)
    Schulte-Merker S
    Development (Cambridge, England) 141.6 (2014 Mar): 1228-38.

    Praxis der Pneumologie
    Bassermann FJ
    Praxis der Pneumologie 26.12 (1972 Dec): 677-87.

    Developmental biology
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Skeletal muscle
    Henry CA
    Skeletal muscle 6. (2016): 18.

    Journal of cell science
    Ashworth R
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5181-90.

    WB References

    The Journal of cell biology
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    The Journal of biological chemistry
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

    The FEBS journal
    Pan H
    The FEBS journal 281.13 (2014 Jul): 2990-3003.

    Development, growth & differentiation
    Grimaldi A
    Development, growth & differentiation 54.4 (2012 May): 489-502.

    American journal of human genetics
    Laing NG
    American journal of human genetics 93.1 (2013 Jul 11): 6-18.

    IHC References

    The Journal of cell biology
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Journal of toxicologic pathology
    Chen YH
    Journal of toxicologic pathology 27.1 (2014 Apr): 19-24.

    Molecules (Basel, Switzerland)
    Chen YH
    Molecules (Basel, Switzerland) 19.1 (2014 Jan 7): 641-50.

    The Journal of biological chemistry
    Yang RB
    The Journal of biological chemistry 289.27 (2014 Jul 4): 18928-42.

    The FEBS journal
    Pan H
    The FEBS journal 281.13 (2014 Jul): 2990-3003.

    Zebrafish
    Kung HN
    Zebrafish 12.1 (2015 Feb): 58-70.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Karlstrom R
    Developmental dynamics : an official publication of the American Association of Anatomists 242.5 (2013 May): 539-49.

    PloS one
    Watabe S
    PloS one 8.12 (2013): e85500.

    Development, growth & differentiation
    Grimaldi A
    Development, growth & differentiation 54.4 (2012 May): 489-502.

    Journal of the peripheral nervous system : JPNS
    Hutson LD
    Journal of the peripheral nervous system : JPNS 17.1 (2012 Mar): 76-89.

    Human molecular genetics
    Kunkel LM
    Human molecular genetics 22.3 (2013 Feb 1): 568-77.

    Journal of applied toxicology : JAT
    Chen YH
    Journal of applied toxicology : JAT 35.3 (2015 Mar): 287-94.

    American journal of human genetics
    Laing NG
    American journal of human genetics 93.1 (2013 Jul 11): 6-18.

    Journal of pediatric gastroenterology and nutrition
    Shiloh H
    Journal of pediatric gastroenterology and nutrition 6.3 (1987 May-Jun): 359-64.

    Anatomical record (Hoboken, N.J. : 2007)
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    The Anatomical record
    Gonyea WJ
    The Anatomical record 228.3 (1990 Nov): 255-61.

    Developmental biology
    Klymkowsky MW
    Developmental biology 136.1 (1989 Nov): 104-17.

    Developmental cell
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Cell reports
    Mercader N
    Cell reports 28.5 (2019 Jul 30): 1296-1306.e6.

    IP References

    The Journal of biological chemistry
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

    Epitope Map References

    The Journal of biological chemistry
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

    All References

    The Journal of cell biology
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Journal of toxicologic pathology
    Chen YH
    Journal of toxicologic pathology 27.1 (2014 Apr): 19-24.

    Molecules (Basel, Switzerland)
    Chen YH
    Molecules (Basel, Switzerland) 19.1 (2014 Jan 7): 641-50.

    The Journal of biological chemistry
    Yang RB
    The Journal of biological chemistry 289.27 (2014 Jul 4): 18928-42.

    The FEBS journal
    Pan H
    The FEBS journal 281.13 (2014 Jul): 2990-3003.

    Zebrafish
    Kung HN
    Zebrafish 12.1 (2015 Feb): 58-70.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Karlstrom R
    Developmental dynamics : an official publication of the American Association of Anatomists 242.5 (2013 May): 539-49.

    PloS one
    Watabe S
    PloS one 8.12 (2013): e85500.

    Development, growth & differentiation
    Grimaldi A
    Development, growth & differentiation 54.4 (2012 May): 489-502.

    Journal of the peripheral nervous system : JPNS
    Hutson LD
    Journal of the peripheral nervous system : JPNS 17.1 (2012 Mar): 76-89.

    Human molecular genetics
    Kunkel LM
    Human molecular genetics 22.3 (2013 Feb 1): 568-77.

    Journal of applied toxicology : JAT
    Chen YH
    Journal of applied toxicology : JAT 35.3 (2015 Mar): 287-94.

    American journal of human genetics
    Laing NG
    American journal of human genetics 93.1 (2013 Jul 11): 6-18.

    Journal of pediatric gastroenterology and nutrition
    Shiloh H
    Journal of pediatric gastroenterology and nutrition 6.3 (1987 May-Jun): 359-64.

    Anatomical record (Hoboken, N.J. : 2007)
    Sanger JM
    Anatomical record (Hoboken, N.J. : 2007) 297.9 (2014 Sep): 1571-84.

    The Anatomical record
    Gonyea WJ
    The Anatomical record 228.3 (1990 Nov): 255-61.

    Developmental biology
    Klymkowsky MW
    Developmental biology 136.1 (1989 Nov): 104-17.

    Developmental cell
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Cell reports
    Mercader N
    Cell reports 28.5 (2019 Jul 30): 1296-1306.e6.

    Journal of cell science
    Goillot E
    Journal of cell science 125.Pt 16 (2012 Aug 15): 3790-800.

    Skeletal muscle
    Miller JB
    Skeletal muscle 3.1 (2013 Dec 6): 28.

    Developmental biology
    Currie PD
    Developmental biology 368.2 (2012 Aug 15): 193-202.

    Molecular biology of the cell
    Du SJ
    Molecular biology of the cell 24.22 (2013 Nov): 3511-21.

    Journal of neuroscience methods
    Feldman EL
    Journal of neuroscience methods 205.2 (2012 Apr 15): 277-82.

    Developmental biology
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    PloS one
    Abdelilah-Seyfried S
    PloS one 7.2 (2012): e31041.

    The Journal of clinical investigation
    Lecker SH
    The Journal of clinical investigation 117.12 (2007 Dec): 3940-51.

    Developmental biology
    Poss KD
    Developmental biology 382.2 (2013 Oct 15): 427-35.

    Molecular and cellular biology
    Jones TI
    Molecular and cellular biology 34.11 (2014 Jun): 1942-55.

    Genes & development
    Kimelman D
    Genes & development 28.4 (2014 Feb 15): 384-95.

    Development (Cambridge, England)
    Poss KD
    Development (Cambridge, England) 140.3 (2013 Feb 1): 660-6.

    BMC biology
    Barresi MJ
    BMC biology 10. (2012 May 4): 40.

    Development (Cambridge, England)
    Schulte-Merker S
    Development (Cambridge, England) 141.6 (2014 Mar): 1228-38.

    Praxis der Pneumologie
    Bassermann FJ
    Praxis der Pneumologie 26.12 (1972 Dec): 677-87.

    Developmental biology
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Skeletal muscle
    Henry CA
    Skeletal muscle 6. (2016): 18.

    Journal of cell science
    Ashworth R
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5181-90.

    The Journal of biological chemistry
    Stockdale FE
    The Journal of biological chemistry 264.22 (1989 Aug 5): 13122-30.

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