15C3
Catalog Fields
Clone ID/Product Name: 15C3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_528211
Clonality: Monoclonal
Immunogen: Manduca ecdysone receptor, only the EcR-B1 portion of the fusion protein injected
Clone:
Immunogen Sequence:
Myeloma Strain: Designation of Fox-NY cells
Epitope Mapped: No
Antigen Name: ecdysone receptor (EcR) (common), Manduca sexta
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/11/1998
Antigen Molecular Weight: 64 kDa (predicted); between 70-80 kDa by immunoblotting
Depositor: Riddiford, L.M.
Antigen Sequence:
Depositor Institution: University of Washington
Antigen Species: Manduca sexta
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bombyx mori, Drosophila, Locusta migratoria, Manduca
Additional Information: Immunization of 100 micrograms on 3/10/95, 4/20/95, 5/3/95, 5/30/95 used complete Freund’s adjuvant (1st); boosts used incomplete Freund’s. RRID:AB_528211
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15C3 was deposited to the DSHB by Riddiford, L.M. (DSHB Hybridoma Product 15C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
2 References
Initial Publication
Cloning of an ecdysone receptor homolog from Manduca sexta and the developmental profile of its mRNA in wings.
Riddiford LM
Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.