Antigen: ecdysone receptor (EcR) (common), Manduca sexta
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Riddiford, L.M.
Host Species: mouse
Depositors Institution: Department of Zoology, University of Washington
Positive Tested Species Reactivity: Bombyx mori, Drosophila, Locusta migratoria, Manduca
Antigen Molecular Weight: 64 kDa (predicted); between 70-80 kDa by immunoblotting
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Manduca ecdysone receptor, only the EcR-B1 portion of the fusion protein injected
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Designation of Fox-NY cells
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_528211
Additional Information: Immunization of 100 micrograms on 3/10/95, 4/20/95, 5/3/95, 5/30/95 used complete Freund’s adjuvant (1st); boosts used incomplete Freund’s. RRID:AB_528211
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15C3 was deposited to the DSHB by Riddiford, L.M. (DSHB Hybridoma Product 15C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Insect biochemistry and molecular biology
Insect biochemistry and molecular biology 25.7 (1995 Jul): 845-56.