C17.9C6

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$40.00
SKU: C17.9C6
View product citations for antibody C17.9C6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Notch, intracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Artavanis-Tsakonas, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 289 kDa; Apparent: 300 kDa and C-terminal fragments of 120 kDa and lower MW, resulting from proteolysis.
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: N
Immunogen: Recombinant fusion protein containing aa 1791-2504 of the intracellular domain of Notch.
Alternate Gene Names: CG3936, Notch, NECD, NICD
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8
Epitope Location or Sequence: intracellular domain, aa 1791-2504.
Uniprot ID: P07207 
Immunogen Sequence: Partial protein
Entrez Gene ID: 31293 
Additional Characterization:
Antibody Registry ID: AB_528410 
Additional Information: The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C17.9C6 was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C17.9C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

20 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    UDP-sugar transporter implicated in glycosylation and processing of Notch.
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Regulation of notch signaling by o-linked fucose.
    Irvine KD
    Cell 111.6 (2002 Dec 13): 893-904.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Evidence for Notch-mediated lateral inhibition in organizing butterfly wing scales.
    Reed RD
    Development genes and evolution 214.1 (2004 Jan): 43-6.

    The involvement of the Notch locus in Drosophila oogenesis.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 115.4 (1992 Aug): 913-22.

    Complex cellular and subcellular regulation of notch expression during embryonic and imaginal development of Drosophila: implications for notch function.
    Artavanis-Tsakonas S
    The Journal of cell biology 113.3 (1991 May): 657-69.

    Regulation of Notch signalling by non-visual beta-arrestin.
    Artavanis-Tsakonas S
    Nature cell biology 7.12 (2005 Dec): 1191-201.

    Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.
    Muskavitch MA
    Development (Cambridge, England) 127.7 (2000 Apr): 1373-85.

    Calcium depletion dissociates and activates heterodimeric notch receptors.
    Aster JC
    Molecular and cellular biology 20.5 (2000 Mar): 1825-35.

    Structural requirements for notch signalling with delta and serrate during the development and patterning of the wing disc of Drosophila.
    Martinez Arias A
    Development (Cambridge, England) 127.14 (2000 Jul): 3185-95.

    Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta.
    Jarman AP
    Development (Cambridge, England) 130.26 (2003 Dec): 6475-84.

    Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA.
    López-Bayghen E
    ASN neuro 5.5 (2013 Dec 23): e00130.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    WB References

    Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila.
    Artavanis-Tsakonas S
    Cell 61.3 (1990 May 4): 523-34.

    UDP-sugar transporter implicated in glycosylation and processing of Notch.
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Indirect evidence for Delta-dependent intracellular processing of notch in Drosophila embryos.
    Schweisguth F
    Current biology : CB 8.13 (1998 Jun 18): 771-4.

    Regulation of Notch signalling by non-visual beta-arrestin.
    Artavanis-Tsakonas S
    Nature cell biology 7.12 (2005 Dec): 1191-201.

    O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors.
    Okajima T
    The Journal of biological chemistry 283.51 (2008 Dec 19): 35486-95.

    Calcium depletion dissociates and activates heterodimeric notch receptors.
    Aster JC
    Molecular and cellular biology 20.5 (2000 Mar): 1825-35.

    Apoptotic activities of wild-type and Alzheimer's disease-related mutant presenilins in Drosophila melanogaster.
    Fortini ME
    The Journal of cell biology 146.6 (1999 Sep 20): 1351-64.

    Methylmercury induces activation of Notch signaling.
    Rand MD
    Neurotoxicology 27.6 (2006 Dec): 982-91.

    Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA.
    López-Bayghen E
    ASN neuro 5.5 (2013 Dec 23): e00130.

    IHC References

    The involvement of the Notch locus in Drosophila oogenesis.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 115.4 (1992 Aug): 913-22.

    Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.
    Muskavitch MA
    Development (Cambridge, England) 127.7 (2000 Apr): 1373-85.

    SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila.
    Mishra RK
    Developmental biology 456.1 (2019 Dec 1): 74-85.

    IP References

    Regulation of Notch signalling by non-visual beta-arrestin.
    Artavanis-Tsakonas S
    Nature cell biology 7.12 (2005 Dec): 1191-201.

    Calcium depletion dissociates and activates heterodimeric notch receptors.
    Aster JC
    Molecular and cellular biology 20.5 (2000 Mar): 1825-35.

    Epitope Map References
    All References

    The involvement of the Notch locus in Drosophila oogenesis.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 115.4 (1992 Aug): 913-22.

    Ligand endocytosis drives receptor dissociation and activation in the Notch pathway.
    Muskavitch MA
    Development (Cambridge, England) 127.7 (2000 Apr): 1373-85.

    SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila.
    Mishra RK
    Developmental biology 456.1 (2019 Dec 1): 74-85.

    UDP-sugar transporter implicated in glycosylation and processing of Notch.
    Hayashi S
    Nature cell biology 3.9 (2001 Sep): 816-22.

    Regulation of notch signaling by o-linked fucose.
    Irvine KD
    Cell 111.6 (2002 Dec 13): 893-904.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

    Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants.
    Fortini ME
    Nature 398.6727 (1999 Apr 8): 525-9.

    Evidence for Notch-mediated lateral inhibition in organizing butterfly wing scales.
    Reed RD
    Development genes and evolution 214.1 (2004 Jan): 43-6.

    Complex cellular and subcellular regulation of notch expression during embryonic and imaginal development of Drosophila: implications for notch function.
    Artavanis-Tsakonas S
    The Journal of cell biology 113.3 (1991 May): 657-69.

    Regulation of Notch signalling by non-visual beta-arrestin.
    Artavanis-Tsakonas S
    Nature cell biology 7.12 (2005 Dec): 1191-201.

    Calcium depletion dissociates and activates heterodimeric notch receptors.
    Aster JC
    Molecular and cellular biology 20.5 (2000 Mar): 1825-35.

    Structural requirements for notch signalling with delta and serrate during the development and patterning of the wing disc of Drosophila.
    Martinez Arias A
    Development (Cambridge, England) 127.14 (2000 Jul): 3185-95.

    Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta.
    Jarman AP
    Development (Cambridge, England) 130.26 (2003 Dec): 6475-84.

    Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA.
    López-Bayghen E
    ASN neuro 5.5 (2013 Dec 23): e00130.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila.
    Artavanis-Tsakonas S
    Cell 61.3 (1990 May 4): 523-34.

    Indirect evidence for Delta-dependent intracellular processing of notch in Drosophila embryos.
    Schweisguth F
    Current biology : CB 8.13 (1998 Jun 18): 771-4.

    O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors.
    Okajima T
    The Journal of biological chemistry 283.51 (2008 Dec 19): 35486-95.

    Apoptotic activities of wild-type and Alzheimer's disease-related mutant presenilins in Drosophila melanogaster.
    Fortini ME
    The Journal of cell biology 146.6 (1999 Sep 20): 1351-64.

    Methylmercury induces activation of Notch signaling.
    Rand MD
    Neurotoxicology 27.6 (2006 Dec): 982-91.

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