15G1a(EcR-A)

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$40.00
SKU: 15G1a(EcR-A)
View product citations for antibody 15G1a(EcR-A) on CiteAb

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Available: 105

DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR-A)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Thummel, C. / Hogness, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence: amino acids 15-152
Host Species: mouse
Depositors Institution: Howard Hughes Medical Institute, University of Utah
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: specific for EcR-A
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_10659792 
Additional Information: The immunogen origin was bacteria with a subcutaneous / intraperitoneal route of administration using complete Freund’s adjuvant / incomplete Freund’s adjuvant.

RRID:AB_10659792
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15G1a(EcR-A) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product 15G1a(EcR-A))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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