15G1a(EcR-A)

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$40.00
SKU: 15G1a(EcR-A)
View product citations for antibody 15G1a(EcR-A) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR-A)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Thummel, C. / Hogness, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence: amino acids 15-152
Host Species: mouse
Depositors Institution: Howard Hughes Medical Institute, University of Utah
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: specific for EcR-A
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10659792 
Additional Information: The immunogen origin was bacteria with a subcutaneous / intraperitoneal route of administration using complete Freund’s adjuvant / incomplete Freund’s adjuvant.

RRID:AB_10659792
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15G1a(EcR-A) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product 15G1a(EcR-A))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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