DDA2.7(EcR common)
Catalog Fields
Clone ID/Product Name: DDA2.7(EcR common)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: EcR
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_10683834
Uniprot ID: P34021
Entrez Gene ID: 35540
Clonality: Monoclonal
Immunogen: TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: ecdysone receptor (EcR common)
Epitope Location or Sequence: aa 335-393
Alternate Antigen Name:
Deposit Date: 11/1/2001
Antigen Molecular Weight: Predicted: 91 kDa (EcR-A), 94 kDa (EcR-B1), 73 kDa (EcR-B2); Apparent; 105 kDa (EcR-A and EcR-B1) and 80 kDa (EcR-B2)
Depositor: Thummel, C. / Hogness, D.
Antigen Sequence:
Depositor Institution: University of Utah
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Amblyomma hebraeum, Drosophila, Lobster
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DDA2.7(EcR common) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product DDA2.7(EcR common))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
Hogness DS
Cell 73.7 (1993 Jul 2): 1323-37.