DDA2.7(EcR common)

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SKU: DDA2.7(EcR common)
View product citations for antibody DDA2.7(EcR common) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR common)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Thummel, C. / Hogness, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Utah
Positive Tested Species Reactivity: Amblyomma hebraeum, Drosophila, Lobster
Depositors Notes:
Antigen Molecular Weight: Predicted: 91 kDa (EcR-A), 94 kDa (EcR-B1), 73 kDa (EcR-B2); Apparent; 105 kDa (EcR-A and EcR-B1) and 80 kDa (EcR-B2)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: EcR
Immunogen: TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).
Alternate Gene Names: NR1H1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: aa 335-393
Uniprot ID: P34021 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35540 
Additional Characterization:  
Antibody Registry ID: AB_10683834 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DDA2.7(EcR common) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product DDA2.7(EcR common))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    Epitope Map References
    Gel Supershift References
    All References

    Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
    Hogness DS
    Cell 73.7 (1993 Jul 2): 1323-37.

    Ecdysone receptor expression and activity in adult Drosophila melanogaster.
    Carney GE
    Journal of insect physiology 57.7 (2011 Jul): 899-907.

    Ecdysone receptor expression in the CNS correlates with stage-specific responses to ecdysteroids during Drosophila and Manduca development.
    Hogness DS
    Development (Cambridge, England) 120.1 (1994 Jan): 219-34.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    DNA-binding properties of Drosophila ecdysone receptor isoforms and their modification by the heterodimerization partner ultraspiracle.
    Spindler-Barth M
    Archives of insect biochemistry and physiology 72.3 (2009 Nov): 172-91.

    Steroid signaling promotes stem cell maintenance in the Drosophila testis.
    Matunis EL
    Developmental biology 394.1 (2014 Oct 1): 129-41.

    Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms.
    Hogness DS
    Cell 91.6 (1997 Dec 12): 777-88.

    The ecdysone receptor and ultraspiracle regulate the timing and progression of ovarian morphogenesis during Drosophila metamorphosis.
    Riddiford LM
    Development genes and evolution 208.6 (1998 Aug): 304-17.

    Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor.
    Truman JW
    Mechanisms of development 120.8 (2003 Aug): 909-18.

    Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes.
    Verrijzer CP
    Molecular and cellular biology 30.3 (2010 Feb): 736-44.

    A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila.
    Bender M
    Development (Cambridge, England) 127.13 (2000 Jul): 2897-905.

    Methylation at lysine 4 of histone H3 in ecdysone-dependent development of Drosophila.
    Mazo A
    Nature 426.6962 (2003 Nov 6): 78-83.

    Differential control of gene activity by isoforms A, B1 and B2 of the Drosophila ecdysone receptor.
    Vögtli M
    European journal of biochemistry 268.6 (2001 Mar): 1811-9.

    Analysis of transcriptional activity mediated by Drosophila melanogaster ecdysone receptor isoforms in a heterologous cell culture system.
    Henrich VC
    Insect molecular biology 15.6 (2006 Dec): 785-95.

    dDOR is an EcR coactivator that forms a feed-forward loop connecting insulin and ecdysone signaling.
    Teleman AA
    Current biology : CB 20.20 (2010 Oct 26): 1799-808.

    Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster.
    Demmers JA
    Proteomics 15.4 (2015 Feb): 725-38.

    The ecdysone response enhancer of the Fbp1 gene of Drosophila melanogaster is a direct target for the EcR/USP nuclear receptor.
    Lepesant JA
    Molecular and cellular biology 14.7 (1994 Jul): 4465-74.

    Characterization of an EcR/USP heterodimer target site that mediates ecdysone responsiveness of the Drosophila Lsp-2 gene.
    Benes H
    Molecular & general genetics : MGG 249.5 (1995 Dec 15): 545-56.

    Programmed cell death in the Drosophila CNS is ecdysone-regulated and coupled with a specific ecdysone receptor isoform.
    Truman JW
    Development (Cambridge, England) 119.4 (1993 Dec): 1251-9.

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