23.4-5

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$40.00
SKU: 23.4-5
View product citations for antibody 23.4-5 on CiteAb

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Available: 44

DSHB Data Sheet

Catalog Fields

Antigen: neuronal marker (TAG-1)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Dodd, J.
Isotype: MIgG1
Depositors Institution: Center for Neurobiology & Behavior, Columbia University
Host Species: mouse
Depositors Notes: Binds to cell surface. Immunohistology: embryonic motor neurons, commissural & DRG neurons.
Positive Tested Species Reactivity: Chicken
Gene: CNTN2
Antigen Molecular Weight: 135 kDa
Clonality: Monoclonal
Immunogen: chick spinal cord membranes, dissected tissue
Epitope Mapped: No
Myeloma Strain: NS-1
Additional Information: Axon-associated cell adhesion molecule (AxCAM) which promotes neurite outgrowth by interaction with the AxCAM L1 (G4) of neuritic membrane. Contributes to the organization of axonal domains at nodes of Ranvier RRID:AB_2083348
Uniprot ID: P28685 
Entrez Gene ID: 419825 
Antibody Registry ID: AB_2083348 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23.4-5 was deposited to the DSHB by Jessell, T.M. / Dodd, J. (DSHB Hybridoma Product 23.4-5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Expression of the cell adhesion molecule axonin-1 in neuromeres of the chicken diencephalon.
    Ast M
    The Journal of comparative neurology 381.2 (1997 May 5): 230-52.

    All References

    Expression of the cell adhesion molecule axonin-1 in neuromeres of the chicken diencephalon.
    Ast M
    The Journal of comparative neurology 381.2 (1997 May 5): 230-52.

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