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$45.00 to $425.00
SKU: 23.4-5
View product citations for antibody 23.4-5 on CiteAb

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Available: 33

DSHB Data Sheet

Catalog Fields

Antigen: neuronal marker (TAG-1)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Dodd, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Columbia University
Positive Tested Species Reactivity: Chicken
Depositors Notes: Binds to cell surface. Immunohistology: embryonic motor neurons, commissural & DRG neurons.
Antigen Molecular Weight: 135 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CNTN2
Immunogen: chick spinal cord membranes, dissected tissue
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P28685 
Immunogen Sequence:
Entrez Gene ID: 419825 
Additional Characterization:  
Antibody Registry ID: AB_2083348 
Additional Information: Axon-associated cell adhesion molecule (AxCAM) which promotes neurite outgrowth by interaction with the AxCAM L1 (G4) of neuritic membrane. Contributes to the organization of axonal domains at nodes of Ranvier RRID:AB_2083348
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23.4-5 was deposited to the DSHB by Jessell, T.M. / Dodd, J. (DSHB Hybridoma Product 23.4-5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Expression of the cell adhesion molecule axonin-1 in neuromeres of the chicken diencephalon.
    Ast M
    The Journal of comparative neurology 381.2 (1997 May 5): 230-52.

    All References

    Expression of the cell adhesion molecule axonin-1 in neuromeres of the chicken diencephalon.
    Ast M
    The Journal of comparative neurology 381.2 (1997 May 5): 230-52.

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