4D7/TAG1

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$40.00
SKU: 4D7/TAG1
View product citations for antibody 4D7/TAG1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neuronal marker (TAG-1)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Yamamoto, M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Tsukuba
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: Blots without reducing agents/ IHC using 4% parafolmadehyde fixation.
Antigen Molecular Weight: Predicted: 135 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Cntn2
Immunogen: Brain (rat fetal) lysate.
Alternate Gene Names: TAG-1, TAG-564, TAG1, Tax
Alternate Antibody Name: Contactin-2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P22063 
Immunogen Sequence:
Entrez Gene ID: 25356 
Additional Characterization:  
Antibody Registry ID: AB_2315433 
Additional Information:
Recommended Applications: FFPE, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D7/TAG1 was deposited to the DSHB by Yamamoto, M. (DSHB Hybridoma Product 4D7/TAG1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FB References
  • All References
  • Initial Publication
    IF References

    Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.
    Jaffrey SR
    Nature neuroscience 9.10 (2006 Oct): 1257-64.

    Cdk5 selectively affects the migration of different populations of neurons in the developing spinal cord.
    Yip JW
    The Journal of comparative neurology 503.2 (2007 Jul 10): 297-307.

    WB References
    IHC References

    Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.
    Jaffrey SR
    Nature neuroscience 9.10 (2006 Oct): 1257-64.

    A role for Nr-CAM in the patterning of binocular visual pathways.
    Sakurai T
    Neuron 50.4 (2006 May 18): 535-47.

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    A novel cAMP-dependent pathway activates neuronal integrin function in retinal neurons.
    Long DA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.5 (2004 Feb 4): 1212-6.

    FFPE References

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    FB References

    Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals.
    Meinl E
    Proceedings of the National Academy of Sciences of the United States of America 106.20 (2009 May 19): 8302-7.

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    All References

    Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.
    Jaffrey SR
    Nature neuroscience 9.10 (2006 Oct): 1257-64.

    A role for Nr-CAM in the patterning of binocular visual pathways.
    Sakurai T
    Neuron 50.4 (2006 May 18): 535-47.

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    A novel cAMP-dependent pathway activates neuronal integrin function in retinal neurons.
    Long DA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.5 (2004 Feb 4): 1212-6.

    Cdk5 selectively affects the migration of different populations of neurons in the developing spinal cord.
    Yip JW
    The Journal of comparative neurology 503.2 (2007 Jul 10): 297-307.

    Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis.
    Bar-Or A
    Annals of neurology 71.5 (2012 May): 601-13.

    Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals.
    Meinl E
    Proceedings of the National Academy of Sciences of the United States of America 106.20 (2009 May 19): 8302-7.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

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