4D7/TAG1

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$40.00
SKU: 4D7/TAG1
View product citations for antibody 4D7/TAG1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neuronal marker (TAG-1)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Yamamoto, M.
Isotype: MIgM
Depositors Institution: University of Tsukuba
Host Species: mouse
Depositors Notes: Blots without reducing agents/ IHC using 4% parafolmadehyde fixation.
Positive Tested Species Reactivity: Mouse, Rat
Gene: Cntn2
Antigen Molecular Weight: Predicted: 135 kDa
Alternate Gene Names: TAG-1, TAG-564, TAG1, Tax
Immunogen: Brain (rat fetal) lysate.
Clonality: Monoclonal
Alternate Antibody Name: Contactin-2
Myeloma Strain: NS-1
Uniprot ID: P22063 
Entrez Gene ID: 25356 
Antibody Registry ID: AB_2315433 
Recommended Applications: FFPE, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D7/TAG1 was deposited to the DSHB by Yamamoto, M. (DSHB Hybridoma Product 4D7/TAG1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FB References
  • All References
  • Initial Publication
    IF References

    Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.
    Jaffrey SR
    Nature neuroscience 9.10 (2006 Oct): 1257-64.

    Cdk5 selectively affects the migration of different populations of neurons in the developing spinal cord.
    Yip JW
    The Journal of comparative neurology 503.2 (2007 Jul 10): 297-307.

    WB References
    IHC References

    Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.
    Jaffrey SR
    Nature neuroscience 9.10 (2006 Oct): 1257-64.

    A role for Nr-CAM in the patterning of binocular visual pathways.
    Sakurai T
    Neuron 50.4 (2006 May 18): 535-47.

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    A novel cAMP-dependent pathway activates neuronal integrin function in retinal neurons.
    Long DA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.5 (2004 Feb 4): 1212-6.

    FFPE References

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    FB References

    Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals.
    Meinl E
    Proceedings of the National Academy of Sciences of the United States of America 106.20 (2009 May 19): 8302-7.

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    All References

    Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.
    Jaffrey SR
    Nature neuroscience 9.10 (2006 Oct): 1257-64.

    A role for Nr-CAM in the patterning of binocular visual pathways.
    Sakurai T
    Neuron 50.4 (2006 May 18): 535-47.

    Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons.
    Masu M
    The Journal of comparative neurology 514.3 (2009 May 20): 215-25.

    A novel cAMP-dependent pathway activates neuronal integrin function in retinal neurons.
    Long DA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.5 (2004 Feb 4): 1212-6.

    Cdk5 selectively affects the migration of different populations of neurons in the developing spinal cord.
    Yip JW
    The Journal of comparative neurology 503.2 (2007 Jul 10): 297-307.

    Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis.
    Bar-Or A
    Annals of neurology 71.5 (2012 May): 601-13.

    Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals.
    Meinl E
    Proceedings of the National Academy of Sciences of the United States of America 106.20 (2009 May 19): 8302-7.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

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