4F2

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DSHB Data Sheet
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Catalog Fields

Clone ID/Product Name: 4F2
Available to For-Profits: Yes
Alternate Antibody Name: LIM/homeobox protein Lhx1, rlim, LIM homeobox protein 1, homeobox protein Lim-1
Gene Symbol: Lhx1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_531784 
Uniprot ID: P63007 
RRID:  
Entrez Gene ID: 257634 
Clonality: Monoclonal
Immunogen: Lim2/LhxV5 (rat); recombinant.
Clone:
Immunogen Sequence: 1-360 aa
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Lim 1+2 / LhxV5
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/9/1997
Antigen Molecular Weight: Predicted: 44.8 kDa
Depositor: Jessell, T.M. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University / HHMI
Antigen Species: Rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Human, Mouse, Rat, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4F2 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 4F2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

32 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
    • Initial Publication

    Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes.
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    IF References

    Identification of a retina-specific Otx2 enhancer element active in immature developing photoreceptors.
    Cepko CL
    Developmental biology 360.1 (2011 Dec 1): 241-55.

    Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation.
    Goulding M
    Development (Cambridge, England) 128.8 (2001 Apr): 1335-46.

    In vivo activation of channelrhodopsin-2 reveals that normal patterns of spontaneous activity are required for motoneuron guidance and maintenance of guidance molecules.
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.31 (2010 Aug 4): 10575-85.

    In vivo neuronal subtype-specific targets of Atoh1 (Math1) in dorsal spinal cord.
    Johnson JE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.30 (2011 Jul 27): 10859-71.

    Topical nitroglycerin for ischemic heart disease.
    Conti R
    JAMA 239.20 (1978 May 19): 2166-7.

    Identification of cerebellin2 in chick and its preferential expression by subsets of developing sensory neurons and their targets in the dorsal horn.
    Honig MG
    The Journal of comparative neurology 518.14 (2010 Jul 15): 2818-40.

    Generation of the podocyte and tubular components of an amniote kidney: timing of specification and a role for Wnt signaling.
    Schultheiss TM
    Development (Cambridge, England) 140.22 (2013 Nov): 4565-73.

    Signals from the ventral midline and isthmus regulate the development of Brn3.0-expressing neurons in the midbrain.
    Turner EE
    Mechanisms of development 105.1-2 (2001 Jul): 129-44.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    WB References

    Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation.
    Hill MM
    Journal of extracellular vesicles 3. (2014): .

    Onecut transcription factors are required for the second phase of development of the A13 dopaminergic nucleus in the mouse.
    Clotman F
    The Journal of comparative neurology 520.7 (2012 May 1): 1424-41.

    IHC References

    Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes.
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.
    Zack DJ
    The Journal of comparative neurology 518.24 (2010 Dec 15): 4938-62.

    Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation.
    Goulding M
    Development (Cambridge, England) 128.8 (2001 Apr): 1335-46.

    Synthesis and chromatographic properties of selenazolidine-2-carboxylic acid (beta-selenaproline).
    Blarzino C
    The Italian journal of biochemistry 28.2 (1979 Mar-Apr): 104-10.

    Basic fibroblast growth factor reduces functional and structural damage in chronic kidney disease.
    Vio CP
    American journal of physiology. Renal physiology 306.4 (2014 Feb 15): F430-41.

    Wild-type but not mutant SOD1 transgenic astrocytes promote the efficient generation of motor neuron progenitors from mouse embryonic stem cells.
    Shaw PJ
    BMC neuroscience 14. (2013 Oct 17): 126.

    Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken.
    Ohuchi H
    Biology open 1.11 (2012 Nov 15): 1083-93.

    Generation of the podocyte and tubular components of an amniote kidney: timing of specification and a role for Wnt signaling.
    Schultheiss TM
    Development (Cambridge, England) 140.22 (2013 Nov): 4565-73.

    FFPE References

    Generation of the podocyte and tubular components of an amniote kidney: timing of specification and a role for Wnt signaling.
    Schultheiss TM
    Development (Cambridge, England) 140.22 (2013 Nov): 4565-73.

    Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2.
    Martin DM
    Molecular and cellular neurosciences 49.1 (2012 Jan): 32-43.

    Signals from the ventral midline and isthmus regulate the development of Brn3.0-expressing neurons in the midbrain.
    Turner EE
    Mechanisms of development 105.1-2 (2001 Jul): 129-44.

    All References

    Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes.
    Pfaff SL
    Cell 79.6 (1994 Dec 16): 957-70.

    Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.
    Zack DJ
    The Journal of comparative neurology 518.24 (2010 Dec 15): 4938-62.

    Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation.
    Goulding M
    Development (Cambridge, England) 128.8 (2001 Apr): 1335-46.

    Synthesis and chromatographic properties of selenazolidine-2-carboxylic acid (beta-selenaproline).
    Blarzino C
    The Italian journal of biochemistry 28.2 (1979 Mar-Apr): 104-10.

    Basic fibroblast growth factor reduces functional and structural damage in chronic kidney disease.
    Vio CP
    American journal of physiology. Renal physiology 306.4 (2014 Feb 15): F430-41.

    Wild-type but not mutant SOD1 transgenic astrocytes promote the efficient generation of motor neuron progenitors from mouse embryonic stem cells.
    Shaw PJ
    BMC neuroscience 14. (2013 Oct 17): 126.

    Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken.
    Ohuchi H
    Biology open 1.11 (2012 Nov 15): 1083-93.

    Generation of the podocyte and tubular components of an amniote kidney: timing of specification and a role for Wnt signaling.
    Schultheiss TM
    Development (Cambridge, England) 140.22 (2013 Nov): 4565-73.

    Identification of a retina-specific Otx2 enhancer element active in immature developing photoreceptors.
    Cepko CL
    Developmental biology 360.1 (2011 Dec 1): 241-55.

    In vivo activation of channelrhodopsin-2 reveals that normal patterns of spontaneous activity are required for motoneuron guidance and maintenance of guidance molecules.
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.31 (2010 Aug 4): 10575-85.

    In vivo neuronal subtype-specific targets of Atoh1 (Math1) in dorsal spinal cord.
    Johnson JE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.30 (2011 Jul 27): 10859-71.

    Topical nitroglycerin for ischemic heart disease.
    Conti R
    JAMA 239.20 (1978 May 19): 2166-7.

    Identification of cerebellin2 in chick and its preferential expression by subsets of developing sensory neurons and their targets in the dorsal horn.
    Honig MG
    The Journal of comparative neurology 518.14 (2010 Jul 15): 2818-40.

    Signals from the ventral midline and isthmus regulate the development of Brn3.0-expressing neurons in the midbrain.
    Turner EE
    Mechanisms of development 105.1-2 (2001 Jul): 129-44.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation.
    Hill MM
    Journal of extracellular vesicles 3. (2014): .

    Onecut transcription factors are required for the second phase of development of the A13 dopaminergic nucleus in the mouse.
    Clotman F
    The Journal of comparative neurology 520.7 (2012 May 1): 1424-41.

    Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2.
    Martin DM
    Molecular and cellular neurosciences 49.1 (2012 Jan): 32-43.

    Hedgehog signaling is directly required for the development of zebrafish dorsal root ganglia neurons.
    Raible DW
    Development (Cambridge, England) 130.22 (2003 Nov): 5351-62.

    Meis1 regulates Foxn4 expression during retinal progenitor cell differentiation.
    Cai L
    Biology open 2.11 (2013): 1125-36.

    Dehydroepiandrosterone biosynthesis, role, and mechanism of action in the developing neural tube.
    Compagnone NA
    Frontiers in endocrinology 3. (2012): 16.

    Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons.
    Price SR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.2 (2012 Jan 11): 490-505.

    Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons.
    Partanen J
    Developmental biology 343.1-2 (2010 Jul 1): 63-70.

    Spatiotemporally restricted regulation of generic motor neuron programs by miR-196-mediated repression of Hoxb8.
    Kessel M
    Developmental biology 344.2 (2010 Aug 15): 857-68.

    Temporal and spatial expression of CCN3 during retina development.
    Marx M
    Developmental neurobiology 72.11 (2012 Nov): 1363-75.

    Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis.
    Trainor PA
    Development (Cambridge, England) 135.4 (2008 Feb): 729-41.

    Analysis of early ventral telencephalic defects in mice lacking functional Gli3 protein.
    Price DJ
    The Journal of comparative neurology 512.5 (2009 Feb 10): 613-27.

    Central and peripheral retina arise through distinct developmental paths.
    Hyer J
    PloS one 8.4 (2013): e61422.

    Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Simeone A
    Development (Cambridge, England) 141.2 (2014 Jan): 377-88.

    Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord.
    Trainor PA
    Development (Cambridge, England) 136.14 (2009 Jul): 2329-34.

    Wnt/β-catenin signaling is disrupted in the extra-toes (Gli3(Xt/Xt) ) mutant from early stages of forebrain development, concomitant with anterior neural plate patterning defects.
    Mason JO
    The Journal of comparative neurology 519.9 (2011 Jun 15): 1640-57.

    A nonclassical bHLH Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling.
    Johnson JE
    Genes & development 22.2 (2008 Jan 15): 166-78.

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