74.5A5

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$40.00
SKU: 74.5A5
View product citations for antibody 74.5A5 on CiteAb

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Available: 773

DSHB Data Sheet

Catalog Fields

Antigen: Nkx2.2 transcription factor
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Apparent: 28-30 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: NKX2-2
Immunogen: Nkx2.2-GST fusion protein expressed in E. coli.
Alternate Gene Names: NK2 homeobox 2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q9YHB7 
Immunogen Sequence:
Entrez Gene ID: 428549 
Additional Characterization:  
Antibody Registry ID: AB_2314952 AB_531794 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
74.5A5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 74.5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

23 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube.
    Nabeshima Y
    Mechanisms of development 113.2 (2002 May): 169-74.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes.
    Whittemore SR
    Experimental neurology 192.1 (2005 Mar): 11-24.

    Tulp3 is a critical repressor of mouse hedgehog signaling.
    Petkovich M
    Developmental dynamics : an official publication of the American Association of Anatomists 238.5 (2009 May): 1140-9.

    DCC mediated axon guidance of spinal interneurons is essential for normal locomotor central pattern generator function.
    Kullander K
    Developmental biology 366.2 (2012 Jun 15): 279-89.

    Small ubiquitin-like Modifier (SUMO) modification inhibits GLI2 protein transcriptional activity in vitro and in vivo.
    Wang B
    The Journal of biological chemistry 287.24 (2012 Jun 8): 20483-9.

    The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling.
    Jackson PK
    Cell 152.1-2 (2013 Jan 17): 210-23.

    Phosphodiesterase 5 inhibition at disease onset prevents experimental autoimmune encephalomyelitis progression through immunoregulatory and neuroprotective actions.
    García A
    Experimental neurology 251. (2014 Jan): 58-71.

    Olig1 is required for noggin-induced neonatal myelin repair.
    Rowitch DH
    Annals of neurology 81.4 (2017 Apr): 560-571.

    Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    Commissural axonal corridors instruct neuronal migration in the mouse spinal cord.
    Wilson SI
    Nature communications 6. (2015 May 11): 7028.

    Epigenetic Modulation of Human Induced Pluripotent Stem Cell Differentiation to Oligodendrocytes.
    Fossati V
    International journal of molecular sciences 17.4 (2016 Apr 22): .

    <i>Dlk1-Dio3</i> locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity.
    Chen JA
    eLife 7. (2018 Oct 12): .

    WB References
    IHC References
    FFPE References
    All References

    The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages.
    Nabeshima Y
    Current biology : CB 12.13 (2002 Jul 9): 1157-63.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

    Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord.
    Yaginuma H
    The Journal of comparative neurology 521.13 (2013 Sep 1): 2987-3002.

    Distinct sites of origin of oligodendrocytes and somatic motoneurons in the chick spinal cord: oligodendrocytes arise from Nkx2.2-expressing progenitors by a Shh-dependent mechanism.
    Cochard P
    Development (Cambridge, England) 128.8 (2001 Apr): 1369-79.

    Tcf7l2/Tcf4 Transcriptional Repressor Function Requires HDAC Activity in the Developing Vertebrate CNS.
    Matise MP
    PloS one 11.9 (2016): e0163267.

    Zika Virus Can Strongly Infect and Disrupt Secondary Organizers in the Ventricular Zone of the Embryonic Chicken Brain.
    Fekete DM
    Cell reports 23.3 (2018 Apr 17): 692-700.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube.
    Nabeshima Y
    Mechanisms of development 113.2 (2002 May): 169-74.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes.
    Whittemore SR
    Experimental neurology 192.1 (2005 Mar): 11-24.

    Tulp3 is a critical repressor of mouse hedgehog signaling.
    Petkovich M
    Developmental dynamics : an official publication of the American Association of Anatomists 238.5 (2009 May): 1140-9.

    DCC mediated axon guidance of spinal interneurons is essential for normal locomotor central pattern generator function.
    Kullander K
    Developmental biology 366.2 (2012 Jun 15): 279-89.

    Small ubiquitin-like Modifier (SUMO) modification inhibits GLI2 protein transcriptional activity in vitro and in vivo.
    Wang B
    The Journal of biological chemistry 287.24 (2012 Jun 8): 20483-9.

    The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling.
    Jackson PK
    Cell 152.1-2 (2013 Jan 17): 210-23.

    Phosphodiesterase 5 inhibition at disease onset prevents experimental autoimmune encephalomyelitis progression through immunoregulatory and neuroprotective actions.
    García A
    Experimental neurology 251. (2014 Jan): 58-71.

    Olig1 is required for noggin-induced neonatal myelin repair.
    Rowitch DH
    Annals of neurology 81.4 (2017 Apr): 560-571.

    Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    Commissural axonal corridors instruct neuronal migration in the mouse spinal cord.
    Wilson SI
    Nature communications 6. (2015 May 11): 7028.

    Epigenetic Modulation of Human Induced Pluripotent Stem Cell Differentiation to Oligodendrocytes.
    Fossati V
    International journal of molecular sciences 17.4 (2016 Apr 22): .

    <i>Dlk1-Dio3</i> locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity.
    Chen JA
    eLife 7. (2018 Oct 12): .

    Expression patterns of Pax6 and Pax7 in the adult brain of a urodele amphibian, Pleurodeles waltl.
    Moreno N
    The Journal of comparative neurology 521.9 (2013 Jun 15): 2088-124.

    Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons.
    Partanen J
    Developmental biology 343.1-2 (2010 Jul 1): 63-70.

    Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling.
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

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