Antigen: Nkx2.2 transcription factor
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Antigen Molecular Weight: Apparent: 28-30 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Nkx2.2-GST fusion protein expressed in E. coli.
Alternate Gene Names: NK2 homeobox 2
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q9YHB7
Entrez Gene ID: 428549
Antibody Registry ID: AB_2314952 AB_531794
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
74.5A5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 74.5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).