Antigen: Tyrosine-protein phosphatase 99A
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody recognizes the native form of DPTP99A and works well for IP, IF and IHC.
Predicted Species Reactivity:
Immunogen: Recombinant protein domain
Alternate Gene Names: Dmel_CG2005
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: HL-1
Epitope Location or Sequence:
Uniprot ID: P35832
Immunogen Sequence: Extracellular domain
Entrez Gene ID: 43469
Antibody Registry ID: AB_528445
Additional Information: RRID:AB_528445. FBgn0004369.
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C71B7 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 4C71B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.