8D12 anti-Repo

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$40.00
SKU: 8D12 anti-Repo
View product citations for antibody 8D12 anti-Repo on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Repo; Reversed polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Excellent marker for glial cells; stains the nuclei of all glia, except for the midline glia
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Repo
Immunogen: Repo (a.a. 218-612)-6Xhistidine fusion protein.
Alternate Gene Names: AbRK2; Repo; REPO; rk2; RK2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q7KSE4 
Immunogen Sequence:
Entrez Gene ID: 47285 
Additional Characterization:
Antibody Registry ID: AB_528448 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8D12 anti-Repo was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 8D12 anti-Repo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

57 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

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