Antigen: Repo; Reversed polarity protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Excellent marker for glial cells; stains the nuclei of all glia, except for the midline glia
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Repo (a.a. 218-612)-6Xhistidine fusion protein.
Alternate Gene Names: AbRK2; Repo; REPO; rk2; RK2
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q7KSE4
Entrez Gene ID: 47285
Antibody Registry ID: AB_528448
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8D12 anti-Repo was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 8D12 anti-Repo)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).