Antigen: fasciclin II (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Antigen Sequence: 103 a.a. at the intracellular C-terminus
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Alternate Gene Names: 1D4; Ab 1D4; anon-EST:Liang-1.60; CG3665; clone 1.60; CT12301; Dmel\CG3665; EG:EG0007.3; Fas; FAS 11; Fas 2; fas II; Fas II; FAS II; fas-II; Fas-II; fas2; FAS2; FascII; fasII; Fasii; FasII; FASII; l(1)G0032; l(1)G0048; l(1)G0081; l(1)G0293; l(1)G0336; mAb 1D4; mAb1D4; mAB1D4; MAB1D4
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3 x 63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: P34082
Entrez Gene ID: 31364
Antibody Registry ID: AB_528235
Additional Information: For experiments involving live cells and tissue, 1D4 anti-Fasciclin II supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or firstname.lastname@example.org for special ordering instructions.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D4 anti-Fasciclin II was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 1D4 anti-Fasciclin II)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).