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$45.00 to $425.00
SKU: 9D82B3
View product citations for antibody 9D82B3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: DLAR
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 9D8 works better for staining in larvae than 8C4 (and as well as 8C4 in embryos), the antibody used in [PMID: 10648238]
Antigen Molecular Weight: Predicted: 226 kDa; Apparent: 240 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Lar
Immunogen: Native DLAR
Alternate Gene Names: CG10443, DLAR, PTP38A
Alternate Antibody Name: 9D8
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: HL-1
Epitope Location or Sequence:
Uniprot ID: P16621 
Immunogen Sequence: Full length protein
Entrez Gene ID: 35259 
Additional Characterization:  
Antibody Registry ID: AB_528202 
Additional Information: DLAR is a neural receptor-linked protein tyrosine phosphatase; also known as Leukocyte-antigen-related-like
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9D82B3 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 9D82B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

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