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SKU: 9D82B3

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 9D82B3
Available to For-Profits: Yes
Alternate Antibody Name: 9D8
Gene Name: Lar
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528202 
Uniprot ID: P16621 
Entrez Gene ID: 35259 
Clonality: Monoclonal
Immunogen: Native DLAR
Immunogen Sequence: Full length protein
Myeloma Strain: HL-1
Epitope Mapped: No
Antigen Name: DLAR
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/12/2005
Antigen Molecular Weight: Predicted: 226 kDa; Apparent: 240 kDa
Depositor: Zinn, K.
Antigen Sequence:
Depositor Institution: California Institute of Technology
Antigen Species: Drosophila
Depositor Notes: 9D8 works better for staining in larvae than 8C4 (and as well as 8C4 in embryos), the antibody used in [PMID: 10648238]
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: DLAR is a neural receptor-linked protein tyrosine phosphatase; also known as Leukocyte-antigen-related-like
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9D82B3 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 9D82B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

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