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$45.00 to $425.00
SKU: 8B22F5
View product citations for antibody 8B22F5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Zinn, K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This mAb differs from other anti-DPTP10D, as it recognizes the native form of DPTP10D and works well for IP. this mAb works much better than 45E10 (a different anti-DPTP10D clone available from DSHB) for staining larval material; the two mAbs are equivalent on embryos.
Antigen Molecular Weight: Predicted: multiple isoforms between 177 and 222 kDa; Apparent: 200 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ptp10D
Immunogen: Recombinant fusion protein containing the extracellular domain of PTP10D produced in monkey COS7 cells.
Alternate Gene Names: CG1817
Alternate Antibody Name: 8B2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P35992 
Immunogen Sequence: Partial protein
Entrez Gene ID: 32115 
Additional Characterization:  
Antibody Registry ID: AB_528443 
Additional Information: PTP10D is a receptor tyrosine phosphatase on motor neurons. 8B22F5stains the tracheal system and the axons of the central nervous system. The specificity of 8B22F5 was tested on Ptp10D mutants by immunoprecipitation and immunohistology.
Recommended Applications: Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8B22F5 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 8B22F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    Epitope Map References
    All References

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