Antibody Name: 8B22F5
Antigen:receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila
Catalog Fields
Identity
Clone ID / Antibody Name: 8B22F5
Alternate Antibody Name: 8B2
Antigen Name: receptor-linked protein tyrosine phosphatase 10D, DPTP10D, Drosophila
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: Ptp10D
Entrez Gene ID: 32115
Antigen Sequence: —
Antigen Species: Drosophila
Antigen Mol. Weight: Predicted: multiple isoforms between 177 and 222 kDa; Apparent: 200 kDa
Uniprot ID: P35992
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: HL-1
Antibody Registry / RRID: AB_528443
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Recombinant fusion protein containing the extracellular domain of PTP10D produced in monkey COS7 cells.
Immunogen Sequence: Partial protein
Epitope Mapped: Yes
Epitope Location / Seq: Extracellular domain
Clone: —
Additional Info: PTP10D is a receptor tyrosine phosphatase on motor neurons. 8B22F5stains the tracheal system and the axons of the central nervous system. The specificity of 8B22F5 was tested on Ptp10D mutants by immunoprecipitation and immunohistology.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Drosophila
Predicted Reactivity: —
Depositor
Depositor Name: K. Zinn
Institution: California Institute of Technology
Deposit Date: June 16, 2005
Depositor Notes: This mAb differs from other anti-DPTP10D, as it recognizes the native form of DPTP10D and works well for IP. this mAb works much better than 45E10 (a different anti-DPTP10D clone available from DSHB) for staining larval material; the two mAbs are equivalent on embryos.
Recommended Applications
IF
IP
Each lab must optimize concentration per application and species. Full usage recommendations →
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