7D6

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$40.00
SKU: 7D6
View product citations for antibody 7D6 on CiteAb

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Available: 107

DSHB Data Sheet

Catalog Fields

Antigen: L-CAM/E-cadherin, chicken
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Gallin, W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Alberta
Positive Tested Species Reactivity: Chicken
Depositors Notes: Does not cross-react with any mammalian species tested, nor with Xenopus laevis, nor with any invertebrate species tested. Doesn't appear to work for western blotting. Doesn't appear to affect L-CAM function. Stains nearly all epithelial cells.
Antigen Molecular Weight: Predicted: 98 kDa; Apparent: 124 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CDH1
Immunogen: 81 kDa N-terminal tryptic fragment of L-CAM from chicken liver plasma membranes.
Alternate Gene Names: Cadherin-1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P08641 
Immunogen Sequence: Partial protein
Entrez Gene ID: 415860 
Additional Characterization:
Antibody Registry ID: AB_528115 
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7D6 was deposited to the DSHB by Gallin, W. (DSHB Hybridoma Product 7D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    Characterization of L-CAM, a major cell adhesion molecule from embryonic liver cells.
    Cunningham BA
    Proceedings of the National Academy of Sciences of the United States of America 80.4 (1983 Feb): 1038-42.

    IF References

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Kinetics of v-src-induced epithelial-mesenchymal transition in developing glandular stomach.
    Iba H
    Oncogene 22.6 (2003 Feb 13): 884-93.

    Characterization of supporting cell phenotype in the avian inner ear: implications for sensory regeneration.
    Warchol ME
    Hearing research 227.1-2 (2007 May): 11-8.

    In vitro self-assembly of proepicardial cell aggregates: an embryonic vasculogenic model for vascular tissue engineering.
    Muñoz-Chápuli R
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 288.7 (2006 Jul): 700-13.

    Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes.
    Corwin JT
    The Journal of comparative neurology 521.6 (2013 Apr 15): 1430-48.

    Characterization of chicken epidermal dendritic cells.
    Magyar A
    Immunology 119.2 (2006 Oct): 278-88.

    Regulation of lens gap junctions by Transforming Growth Factor beta.
    Musil LS
    Molecular biology of the cell 21.10 (2010 May 15): 1686-97.

    IHC References
    IP References
    FACS References
    All References

    Structural variants of the neural cell adhesion molecule (N-CAM) in developing feathers.
    Gallin WJ
    Developmental biology 150.1 (1992 Mar): 171-84.

    Adherens junction breakdown in the periderm following cadmium administration in the chick embryo: distribution of cadherins and associated molecules.
    Bannigan J
    Reproductive toxicology (Elmsford, N.Y.) 25.1 (2008 Jan): 39-46.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Kinetics of v-src-induced epithelial-mesenchymal transition in developing glandular stomach.
    Iba H
    Oncogene 22.6 (2003 Feb 13): 884-93.

    Characterization of supporting cell phenotype in the avian inner ear: implications for sensory regeneration.
    Warchol ME
    Hearing research 227.1-2 (2007 May): 11-8.

    In vitro self-assembly of proepicardial cell aggregates: an embryonic vasculogenic model for vascular tissue engineering.
    Muñoz-Chápuli R
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 288.7 (2006 Jul): 700-13.

    Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes.
    Corwin JT
    The Journal of comparative neurology 521.6 (2013 Apr 15): 1430-48.

    Characterization of chicken epidermal dendritic cells.
    Magyar A
    Immunology 119.2 (2006 Oct): 278-88.

    Regulation of lens gap junctions by Transforming Growth Factor beta.
    Musil LS
    Molecular biology of the cell 21.10 (2010 May 15): 1686-97.

    Similarities in structure and expression between mouse P-cadherin, chicken B-cadherin and frog XB/U-cadherin.
    Müller HA
    Cell adhesion and communication 2.6 (1994 Dec): 511-20.

    Prototypical type I E-cadherin and type II cadherin-7 mediate very distinct adhesiveness through their extracellular domains.
    Dufour S
    The Journal of biological chemistry 281.5 (2006 Feb 3): 2901-10.

    Expression of Toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to Salmonella carrier state.
    Velge P
    Infection and immunity 79.8 (2011 Aug): 3445-54.

    Susceptibility to Salmonella carrier-state: a possible Th2 response in susceptible chicks.
    Velge P
    Veterinary immunology and immunopathology 159.1-2 (2014 May 15): 16-28.

    Characterization of L-CAM, a major cell adhesion molecule from embryonic liver cells.
    Cunningham BA
    Proceedings of the National Academy of Sciences of the United States of America 80.4 (1983 Feb): 1038-42.

    α-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength.
    Dufour S
    The Journal of biological chemistry 288.7 (2013 Feb 15): 4957-69.

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