86f7

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$40.00
SKU: 86f7
View product citations for antibody 86f7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cation-independent mannose-6-phosphate receptor (IGF2R)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Messner, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Bastyr University
Positive Tested Species Reactivity: Bovine, Mouse
Depositors Notes: 86f7 doesn't recognize the human IGF2R. Use non-reducing conditions for western blotting.
Antigen Molecular Weight: Predicted: 270 kDa; Apparent: 300 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: IGF2R
Immunogen: Mannose 6-phosphate receptors mix purified from bovine calf liver.
Alternate Gene Names: CD222, CI-MPR, M6PR, MPR 300, IGF-IIR
Alternate Antibody Name: DM86f7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653.3
Epitope Location or Sequence: Extracellular region
Uniprot ID: P08169 
Immunogen Sequence: Full length protein
Entrez Gene ID: 281849 
Additional Characterization:
Antibody Registry ID: AB_528348 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
86f7 was deposited to the DSHB by Messner, D. (DSHB Hybridoma Product 86f7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    Basolateral sorting signal of the 300-kDa mannose 6-phosphate receptor.
    Dahms NM
    American journal of physiology. Gastrointestinal and liver physiology 282.1 (2002 Jan): G51-60.

    Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes.
    Maxfield FR
    Molecular biology of the cell 15.2 (2004 Feb): 721-33.

    Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons.
    Sheng ZH
    Neuron 68.1 (2010 Oct 6): 73-86.

    T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.
    Maki RA
    PloS one 11.2 (2016): e0149391.

    Golgi spectrin: identification of an erythroid beta-spectrin homolog associated with the Golgi complex.
    Nelson WJ
    The Journal of cell biology 127.3 (1994 Nov): 707-23.

    Role of an acidic cluster/dileucine motif in cation-independent mannose 6-phosphate receptor traffic.
    Maxfield FR
    Traffic (Copenhagen, Denmark) 8.4 (2007 Apr): 402-13.

    Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network.
    Coudrier E
    Nature cell biology 13.7 (2011 Jun 12): 779-89.

    WB References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    Basolateral sorting signal of the 300-kDa mannose 6-phosphate receptor.
    Dahms NM
    American journal of physiology. Gastrointestinal and liver physiology 282.1 (2002 Jan): G51-60.

    Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons.
    Sheng ZH
    Neuron 68.1 (2010 Oct 6): 73-86.

    IP References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    Basolateral sorting signal of the 300-kDa mannose 6-phosphate receptor.
    Dahms NM
    American journal of physiology. Gastrointestinal and liver physiology 282.1 (2002 Jan): G51-60.

    ELISA References
    Epitope Map References
    All References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    Basolateral sorting signal of the 300-kDa mannose 6-phosphate receptor.
    Dahms NM
    American journal of physiology. Gastrointestinal and liver physiology 282.1 (2002 Jan): G51-60.

    Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes.
    Maxfield FR
    Molecular biology of the cell 15.2 (2004 Feb): 721-33.

    Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons.
    Sheng ZH
    Neuron 68.1 (2010 Oct 6): 73-86.

    T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.
    Maki RA
    PloS one 11.2 (2016): e0149391.

    Golgi spectrin: identification of an erythroid beta-spectrin homolog associated with the Golgi complex.
    Nelson WJ
    The Journal of cell biology 127.3 (1994 Nov): 707-23.

    Role of an acidic cluster/dileucine motif in cation-independent mannose 6-phosphate receptor traffic.
    Maxfield FR
    Traffic (Copenhagen, Denmark) 8.4 (2007 Apr): 402-13.

    Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network.
    Coudrier E
    Nature cell biology 13.7 (2011 Jun 12): 779-89.

    Mutational analysis of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor. A consensus casein kinase II site followed by 2 leucines near the carboxyl terminus is important for intracellular targeting of lysosomal enzymes.
    Lobel P
    The Journal of biological chemistry 268.30 (1993 Oct 25): 22338-46.

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