H4B4

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$45.00 to $75.00
SKU: H4B4
View product citations for antibody H4B4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: LAMP-2 (human)
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: August, J.T. / Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: Fusion: 1983
Antigen Molecular Weight: Predicted: 42 kDa; Apparent: 120 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: LAMP2
Immunogen: Human adherent peripheral blood cells
Alternate Gene Names: CD107b, LAMPB, LGP110
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence: Luminal region of LAMP-2
Uniprot ID: P13473 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3920 
Additional Characterization:  
Antibody Registry ID: AB_528129 
Additional Information: LAMP2 is a marker of lysosmes and late endosomes. H4B4 has been reported to recognize all three LAMP-2 isoforms A, B, and C [PMID: 22365987], suggesting that the epitope is located on the luminal region of LAMP-2 common to all isoforms. This antibody induces apoptosis of human microvascular endothelium in vitro [PMID: 18836458].
RRID:AB_528129
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H4B4 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H4B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Purification and characterization of human lysosomal membrane glycoproteins.
    August JT
    Archives of biochemistry and biophysics 268.1 (1989 Jan): 360-78.

    IF References

    Purification and characterization of human lysosomal membrane glycoproteins.
    August JT
    Archives of biochemistry and biophysics 268.1 (1989 Jan): 360-78.

    Trafficking of cell-surface amyloid beta-protein precursor. II. Endocytosis, recycling and lysosomal targeting detected by immunolocalization.
    Selkoe DJ
    Journal of cell science 109 ( Pt 5). (1996 May): 999-1008.

    The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes.
    Tolkovsky AM
    Autophagy 1.1 (2005 Apr): 23-36.

    Generalized lysosome-associated membrane protein-2 defect explains multisystem clinical involvement and allows leukocyte diagnostic screening in Danon disease.
    Angelini C
    The American journal of pathology 168.4 (2006 Apr): 1309-20.

    Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
    Hirano M
    Nature 406.6798 (2000 Aug 24): 906-10.

    Danon disease: intrafamilial phenotypic variability related to a novel LAMP-2 mutation.
    Labarthe F
    Journal of inherited metabolic disease 34.2 (2011 Apr): 515-22.

    Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.
    Schmidt J
    Experimental cell research 316.12 (2010 Jul 15): 1914-24.

    Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor.
    Weissman AM
    The Journal of biological chemistry 283.32 (2008 Aug 8): 22166-76.

    Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
    Coninck SW
    International journal of cancer 75.1 (1998 Jan 5): 105-11.

    Autophagy mediates transporter associated with antigen processing-independent presentation of viral epitopes through MHC class I pathway.
    Khanna R
    Blood 120.5 (2012 Aug 2): 994-1004.

    Methods to Detect Loss of Lysosomal Membrane Integrity.
    Aits S
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 315-329.

    Treatment of rat thyrocytes in vitro with cathepsin B and L inhibitors results in disruption of primary cilia leading to redistribution of the trace amine associated receptor 1 to the endoplasmic reticulum.
    Brix K
    Biochimie 166. (2019 Nov): 270-285.

    Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.
    Taniguchi N
    The Journal of biological chemistry 296. (2021 Jan-Jun): 100354.

    WB References

    Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.
    Wang Y
    Molecular biology of the cell 28.21 (2017 Oct 15): 2833-2842.

    Reduced glucocerebrosidase is associated with increased α-synuclein in sporadic Parkinson's disease.
    Halliday GM
    Brain : a journal of neurology 137.Pt 3 (2014 Mar): 834-48.

    Generalized lysosome-associated membrane protein-2 defect explains multisystem clinical involvement and allows leukocyte diagnostic screening in Danon disease.
    Angelini C
    The American journal of pathology 168.4 (2006 Apr): 1309-20.

    A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome.
    Miranda MC
    Human molecular genetics 17.14 (2008 Jul 15): 2238-43.

    Glucocerebrosidase mutations alter the endoplasmic reticulum and lysosomes in Lewy body disease.
    Morris CM
    Journal of neurochemistry 123.2 (2012 Oct): 298-309.

    Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
    Hirano M
    Nature 406.6798 (2000 Aug 24): 906-10.

    Danon disease: a novel LAMP2 mutation affecting the pre-mRNA splicing and causing aberrant transcripts and partial protein expression.
    Mora M
    Neuromuscular disorders : NMD 18.12 (2008 Dec): 962-6.

    Danon disease: intrafamilial phenotypic variability related to a novel LAMP-2 mutation.
    Labarthe F
    Journal of inherited metabolic disease 34.2 (2011 Apr): 515-22.

    Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.
    Schmidt J
    Experimental cell research 316.12 (2010 Jul 15): 1914-24.

    Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells.
    Kakehi K
    Journal of pharmaceutical and biomedical analysis 70. (2012 Nov): 718-26.

    IHC References
    IP References

    Purification and characterization of human lysosomal membrane glycoproteins.
    August JT
    Archives of biochemistry and biophysics 268.1 (1989 Jan): 360-78.

    Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells.
    Kakehi K
    Journal of pharmaceutical and biomedical analysis 70. (2012 Nov): 718-26.

    FFPE References
    FB References

    Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis.
    Kerjaschki D
    Nature medicine 14.10 (2008 Oct): 1088-96.

    FACS References

    Purification and characterization of human lysosomal membrane glycoproteins.
    August JT
    Archives of biochemistry and biophysics 268.1 (1989 Jan): 360-78.

    Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
    Coninck SW
    International journal of cancer 75.1 (1998 Jan 5): 105-11.

    Protein targeting by tyrosine- and di-leucine-based signals: evidence for distinct saturable components.
    Bonifacino JS
    The Journal of cell biology 135.2 (1996 Oct): 341-54.

    Immortalisation of human bone marrow endothelial cells: characterisation of new cell lines.
    van der Schoot CE
    European journal of clinical investigation 30.7 (2000 Jul): 618-29.

    Epitope Map References
    All References

    Purification and characterization of human lysosomal membrane glycoproteins.
    August JT
    Archives of biochemistry and biophysics 268.1 (1989 Jan): 360-78.

    Danon disease: a novel LAMP2 mutation affecting the pre-mRNA splicing and causing aberrant transcripts and partial protein expression.
    Mora M
    Neuromuscular disorders : NMD 18.12 (2008 Dec): 962-6.

    Danon disease: intrafamilial phenotypic variability related to a novel LAMP-2 mutation.
    Labarthe F
    Journal of inherited metabolic disease 34.2 (2011 Apr): 515-22.

    Influences of the lysosomal associated membrane proteins (Lamp-1, Lamp-2) and Mac-2 binding protein (Mac-2-BP) on the prognosis of pancreatic carcinoma.
    Büchler MW
    Cancer 94.1 (2002 Jan 1): 228-39.

    Treatment of rat thyrocytes in vitro with cathepsin B and L inhibitors results in disruption of primary cilia leading to redistribution of the trace amine associated receptor 1 to the endoplasmic reticulum.
    Brix K
    Biochimie 166. (2019 Nov): 270-285.

    Juvenile-onset acid maltase deficiency with unusual familial features.
    Miranda AF
    Neurology 36.6 (1986 Jun): 818-22.

    Trafficking of cell-surface amyloid beta-protein precursor. II. Endocytosis, recycling and lysosomal targeting detected by immunolocalization.
    Selkoe DJ
    Journal of cell science 109 ( Pt 5). (1996 May): 999-1008.

    The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes.
    Tolkovsky AM
    Autophagy 1.1 (2005 Apr): 23-36.

    Generalized lysosome-associated membrane protein-2 defect explains multisystem clinical involvement and allows leukocyte diagnostic screening in Danon disease.
    Angelini C
    The American journal of pathology 168.4 (2006 Apr): 1309-20.

    Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
    Hirano M
    Nature 406.6798 (2000 Aug 24): 906-10.

    Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.
    Schmidt J
    Experimental cell research 316.12 (2010 Jul 15): 1914-24.

    Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor.
    Weissman AM
    The Journal of biological chemistry 283.32 (2008 Aug 8): 22166-76.

    Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.
    Coninck SW
    International journal of cancer 75.1 (1998 Jan 5): 105-11.

    Autophagy mediates transporter associated with antigen processing-independent presentation of viral epitopes through MHC class I pathway.
    Khanna R
    Blood 120.5 (2012 Aug 2): 994-1004.

    Methods to Detect Loss of Lysosomal Membrane Integrity.
    Aits S
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 315-329.

    Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.
    Taniguchi N
    The Journal of biological chemistry 296. (2021 Jan-Jun): 100354.

    Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.
    Wang Y
    Molecular biology of the cell 28.21 (2017 Oct 15): 2833-2842.

    Reduced glucocerebrosidase is associated with increased α-synuclein in sporadic Parkinson's disease.
    Halliday GM
    Brain : a journal of neurology 137.Pt 3 (2014 Mar): 834-48.

    A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome.
    Miranda MC
    Human molecular genetics 17.14 (2008 Jul 15): 2238-43.

    Glucocerebrosidase mutations alter the endoplasmic reticulum and lysosomes in Lewy body disease.
    Morris CM
    Journal of neurochemistry 123.2 (2012 Oct): 298-309.

    Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells.
    Kakehi K
    Journal of pharmaceutical and biomedical analysis 70. (2012 Nov): 718-26.

    Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis.
    Kerjaschki D
    Nature medicine 14.10 (2008 Oct): 1088-96.

    Protein targeting by tyrosine- and di-leucine-based signals: evidence for distinct saturable components.
    Bonifacino JS
    The Journal of cell biology 135.2 (1996 Oct): 341-54.

    Immortalisation of human bone marrow endothelial cells: characterisation of new cell lines.
    van der Schoot CE
    European journal of clinical investigation 30.7 (2000 Jul): 618-29.

    Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency.
    Sikora J
    Gene 498.2 (2012 May 1): 183-95.

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