H4B4
Catalog Fields
Clone ID/Product Name: H4B4
Available to For-Profits: Yes
Alternate Antibody Name: CD107b
Gene Symbol: LAMP2
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 3920
Clonality: Monoclonal
Immunogen: Human adherent peripheral blood cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X653-Ag8
Epitope Mapped: Yes
Antigen Name: LAMP-2 (human)
Epitope Location or Sequence: Luminal region of LAMP-2
Alternate Antigen Name:
Deposit Date: 4/12/1993
Antigen Molecular Weight: Predicted: 42 kDa; Apparent: 120 kDa
Depositor: August, J.T. / Hildreth, J.E.K.
Antigen Sequence:
Depositor Institution: Johns Hopkins University School of Medicine
Antigen Species: Human
Depositor Notes: Fusion: 1983
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: LAMP2 is a marker of lysosmes and late endosomes. H4B4 has been reported to recognize all three LAMP-2 isoforms A, B, and C [PMID: 22365987], suggesting that the epitope is located on the luminal region of LAMP-2 common to all isoforms. This antibody induces apoptosis of human microvascular endothelium in vitro [PMID: 18836458].
RRID:AB_528129
RRID:AB_528129
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H4B4 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H4B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
25 References
Initial Publication
Purification and characterization of human lysosomal membrane glycoproteins.
August JT
Archives of biochemistry and biophysics 268.1 (1989 Jan): 360-78.