H4A3

(0) No Reviews yet
$40.00
SKU: H4A3
View product citations for antibody H4A3 on CiteAb

In Stock

Available: 952

DSHB Data Sheet

Catalog Fields

Antigen: LAMP-1 (human)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: August, J.T. / Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Hamster, Human, Primate, Rat
Depositors Notes: This antibody recognizes a membrane glycoprotein which interacts with selectins.
Antigen Molecular Weight: Predicted: 42 kDa; Apparent: 120 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LAMP1
Immunogen: Adherent spleen cells
Alternate Gene Names: CD107a, LGP120, LAMPA
Alternate Antibody Name: CD107a
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence: Luminal region
Uniprot ID: P11279 
Immunogen Sequence:
Entrez Gene ID: 3916 
Additional Characterization:
Antibody Registry ID: AB_2296838 
Additional Information: For experiments involving live cells and tissue, H4A3 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H4A3 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H4A3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

31 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    IF References

    A screen of Coxiella burnetii mutants reveals important roles for Dot/Icm effectors and host autophagy in vacuole biogenesis.
    Roy CR
    PLoS pathogens 10.7 (2014 Jul): e1004286.

    Cathepsin L inhibition by the small molecule KGP94 suppresses tumor microenvironment enhanced metastasis associated cell functions of prostate and breast cancer cells.
    Siemann DW
    Clinical and experimental metastasis 30.7 (2013 Oct): 891-902.

    The Francisella tularensis migR, trmE, and cphA genes contribute to F. tularensis pathogenicity island gene regulation and intracellular growth by modulation of the stress alarmone ppGpp.
    Jones BD
    Infection and immunity 81.8 (2013 Aug): 2800-11.

    Extracellular amastigotes of Trypanosoma cruzi are potent inducers of phagocytosis in mammalian cells.
    Mortara RA
    Cellular microbiology 15.6 (2013 Jun): 977-91.

    An extended proteome map of the lysosomal membrane reveals novel potential transporters.
    Journet A
    Molecular and cellular proteomics : MCP 12.6 (2013 Jun): 1572-88.

    A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication.
    Czibener C
    Infection and immunity 81.3 (2013 Mar): 956-64.

    Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus.
    Briones G
    Infection and immunity 81.2 (2013 Feb): 521-30.

    Hypoxia counteracts taxol-induced apoptosis in MDA-MB-231 breast cancer cells: role of autophagy and JNK activation.
    Michiels C
    Cell death and disease 4. (2013 May 16): e638.

    Host pathways important for Coxiella burnetii infection revealed by genome-wide RNA interference screening.
    Roy CR
    mBio 4.1 (2013 Jan 29): e00606-12.

    Ryanodine receptor antagonists adapt NPC1 proteostasis to ameliorate lipid storage in Niemann-Pick type C disease fibroblasts.
    Lieberman AP
    Human molecular genetics 21.14 (2012 Jul 15): 3205-14.

    Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole.
    Roy CR
    mBio 7.4 (2016 Jul 19): .

    Role of SMURF1 ubiquitin ligase in BMP receptor trafficking and signaling.
    Etlinger JD
    Cellular signalling 54. (2019 Feb): 139-149.

    Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research.
    Lane JD
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 257-280.

    Brucella abortus Infection of Placental Trophoblasts Triggers Endoplasmic Reticulum Stress-Mediated Cell Death and Fetal Loss via Type IV Secretion System-Dependent Activation of CHOP.
    Tsolis RM
    mBio 10.4 (2019 Jul 23): .

    A role for the Salmonella Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells.
    Steele-Mortimer O
    Molecular microbiology 112.4 (2019 Oct): 1270-1283.

    WB References

    Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.
    Wang Y
    Molecular biology of the cell 28.21 (2017 Oct 15): 2833-2842.

    Chemical structure requirements and cellular targeting of microRNA-122 by peptide nucleic acids anti-miRs.
    Gait MJ
    Nucleic acids research 40.5 (2012 Mar): 2152-67.

    LAMP2 microdeletions in patients with Danon disease.
    Vatta M
    Circulation. Cardiovascular genetics 3.2 (2010 Apr): 129-37.

    Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
    Yin XM
    The Journal of biological chemistry 285.2 (2010 Jan 8): 1371-83.

    The Salmonella effector SptP dephosphorylates host AAA+ ATPase VCP to promote development of its intracellular replicative niche.
    Koronakis V
    Cell host and microbe 5.3 (2009 Mar 19): 225-33.

    Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptor.
    Cutler DF
    Traffic (Copenhagen, Denmark) 9.11 (2008 Nov): 1905-14.

    Generalized lysosome-associated membrane protein-2 defect explains multisystem clinical involvement and allows leukocyte diagnostic screening in Danon disease.
    Angelini C
    The American journal of pathology 168.4 (2006 Apr): 1309-20.

    Huntingtin-associated protein 1 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking.
    Li L
    The Journal of biological chemistry 277.31 (2002 Aug 2): 28212-21.

    The Exocytosis-regulatory protein synaptotagmin VII mediates cell invasion by Trypanosoma cruzi.
    Andrews NW
    The Journal of experimental medicine 193.9 (2001 May 7): 1097-104.

    PDZ domain protein GIPC interacts with the cytoplasmic tail of melanosomal membrane protein gp75 (tyrosinase-related protein-1).
    Setaluri V
    The Journal of biological chemistry 276.38 (2001 Sep 21): 35768-77.

    IHC References
    Epitope Map References
    All References

    Expression of the lysosomal-associated membrane protein-1 (LAMP-1) in astrocytomas.
    Kristensen B
    International journal of clinical and experimental pathology 6.7 (2013): 1294-305.

    Prosaposin deficiency -- a rarely diagnosed, rapidly progressing, neonatal neurovisceral lipid storage disease. Report of a further patient.
    Harzer K
    Neuropediatrics 36.3 (2005 Jun): 171-80.

    Skin fibroblasts of patients with geleophysic dysplasia due to FBN1 mutations have lysosomal inclusions and losartan improves their microfibril deposition defect.
    Brunetti-Pierri N
    Molecular genetics and genomic medicine 7.9 (2019 Sep): e844.

    A screen of Coxiella burnetii mutants reveals important roles for Dot/Icm effectors and host autophagy in vacuole biogenesis.
    Roy CR
    PLoS pathogens 10.7 (2014 Jul): e1004286.

    Cathepsin L inhibition by the small molecule KGP94 suppresses tumor microenvironment enhanced metastasis associated cell functions of prostate and breast cancer cells.
    Siemann DW
    Clinical and experimental metastasis 30.7 (2013 Oct): 891-902.

    The Francisella tularensis migR, trmE, and cphA genes contribute to F. tularensis pathogenicity island gene regulation and intracellular growth by modulation of the stress alarmone ppGpp.
    Jones BD
    Infection and immunity 81.8 (2013 Aug): 2800-11.

    Extracellular amastigotes of Trypanosoma cruzi are potent inducers of phagocytosis in mammalian cells.
    Mortara RA
    Cellular microbiology 15.6 (2013 Jun): 977-91.

    An extended proteome map of the lysosomal membrane reveals novel potential transporters.
    Journet A
    Molecular and cellular proteomics : MCP 12.6 (2013 Jun): 1572-88.

    A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication.
    Czibener C
    Infection and immunity 81.3 (2013 Mar): 956-64.

    Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus.
    Briones G
    Infection and immunity 81.2 (2013 Feb): 521-30.

    Hypoxia counteracts taxol-induced apoptosis in MDA-MB-231 breast cancer cells: role of autophagy and JNK activation.
    Michiels C
    Cell death and disease 4. (2013 May 16): e638.

    Host pathways important for Coxiella burnetii infection revealed by genome-wide RNA interference screening.
    Roy CR
    mBio 4.1 (2013 Jan 29): e00606-12.

    Ryanodine receptor antagonists adapt NPC1 proteostasis to ameliorate lipid storage in Niemann-Pick type C disease fibroblasts.
    Lieberman AP
    Human molecular genetics 21.14 (2012 Jul 15): 3205-14.

    Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing Constitutive Fusion of Autophagosomes with the Coxiella-Containing Vacuole.
    Roy CR
    mBio 7.4 (2016 Jul 19): .

    Role of SMURF1 ubiquitin ligase in BMP receptor trafficking and signaling.
    Etlinger JD
    Cellular signalling 54. (2019 Feb): 139-149.

    Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research.
    Lane JD
    Methods in molecular biology (Clifton, N.J.) 1880. (2019): 257-280.

    Brucella abortus Infection of Placental Trophoblasts Triggers Endoplasmic Reticulum Stress-Mediated Cell Death and Fetal Loss via Type IV Secretion System-Dependent Activation of CHOP.
    Tsolis RM
    mBio 10.4 (2019 Jul 23): .

    A role for the Salmonella Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells.
    Steele-Mortimer O
    Molecular microbiology 112.4 (2019 Oct): 1270-1283.

    Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.
    Wang Y
    Molecular biology of the cell 28.21 (2017 Oct 15): 2833-2842.

    Chemical structure requirements and cellular targeting of microRNA-122 by peptide nucleic acids anti-miRs.
    Gait MJ
    Nucleic acids research 40.5 (2012 Mar): 2152-67.

    LAMP2 microdeletions in patients with Danon disease.
    Vatta M
    Circulation. Cardiovascular genetics 3.2 (2010 Apr): 129-37.

    Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
    Yin XM
    The Journal of biological chemistry 285.2 (2010 Jan 8): 1371-83.

    The Salmonella effector SptP dephosphorylates host AAA+ ATPase VCP to promote development of its intracellular replicative niche.
    Koronakis V
    Cell host and microbe 5.3 (2009 Mar 19): 225-33.

    Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptor.
    Cutler DF
    Traffic (Copenhagen, Denmark) 9.11 (2008 Nov): 1905-14.

    Generalized lysosome-associated membrane protein-2 defect explains multisystem clinical involvement and allows leukocyte diagnostic screening in Danon disease.
    Angelini C
    The American journal of pathology 168.4 (2006 Apr): 1309-20.

    Huntingtin-associated protein 1 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking.
    Li L
    The Journal of biological chemistry 277.31 (2002 Aug 2): 28212-21.

    The Exocytosis-regulatory protein synaptotagmin VII mediates cell invasion by Trypanosoma cruzi.
    Andrews NW
    The Journal of experimental medicine 193.9 (2001 May 7): 1097-104.

    PDZ domain protein GIPC interacts with the cytoplasmic tail of melanosomal membrane protein gp75 (tyrosinase-related protein-1).
    Setaluri V
    The Journal of biological chemistry 276.38 (2001 Sep 21): 35768-77.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes.
    Andrews NW
    Cell 106.2 (2001 Jul 27): 157-69.

    Fibrin deposited in the Alzheimer's disease brain promotes neuronal degeneration.
    Strickland S
    Neurobiology of aging 36.2 (2015 Feb): 608-17.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review