22d4

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$40.00
SKU: 22d4
View product citations for antibody 22d4 on CiteAb

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Available: 170

DSHB Data Sheet

Catalog Fields

Antigen: mannose 6-phosphate receptor, cation-dependent (M6PR)
Hybridoma Cells Available: Yes
Antigen Species: bovine
Depositor: Messner, D.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Bastyr University
Positive Tested Species Reactivity: Bovine, Human
Depositors Notes: Use non-reducing conditions for western blotting. 22d4 doesn't recognize the murine M6PR.
Antigen Molecular Weight: Predicted: 28 kDa; Apparent: 46 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: M6PR
Immunogen: Mannose 6-phosphate receptors mix (MPR) purified from bovine calf liver
Alternate Gene Names: CD-MPR, MPR 46
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653.3
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P11456 
Immunogen Sequence:
Entrez Gene ID: 281291 
Additional Characterization:
Antibody Registry ID: AB_528347 
Additional Information: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22d4 was deposited to the DSHB by Messner, D. (DSHB Hybridoma Product 22d4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins.
    Munro S
    Science (New York, N.Y.) 346.6209 (2014 Oct 31): 1256898.

    A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes.
    Kornfeld S
    The Journal of cell biology 130.6 (1995 Sep): 1297-306.

    Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor.
    Rohrer J
    The Journal of biological chemistry 278.27 (2003 Jul 4): 24753-8.

    The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding.
    Rohrer J
    The Journal of biological chemistry 279.22 (2004 May 28): 23542-9.

    Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells.
    Waguri S
    Archives of histology and cytology 70.5 (2007 Dec): 303-12.

    HPS6 interacts with dynactin p150Glued to mediate retrograde trafficking and maturation of lysosomes.
    Liu JJ
    Journal of cell science 127.Pt 21 (2014 Nov 1): 4574-88.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    WB References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.
    Maki RA
    PloS one 11.2 (2016): e0149391.

    A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes.
    Kornfeld S
    The Journal of cell biology 130.6 (1995 Sep): 1297-306.

    Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its cytoplasmic tail.
    Rohrer J
    Proceedings of the National Academy of Sciences of the United States of America 94.26 (1997 Dec 23): 14471-6.

    Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.
    Nixon RA
    The Journal of biological chemistry 277.7 (2002 Feb 15): 5299-307.

    Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor.
    Rohrer J
    The Journal of biological chemistry 278.27 (2003 Jul 4): 24753-8.

    The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding.
    Rohrer J
    The Journal of biological chemistry 279.22 (2004 May 28): 23542-9.

    A di-aromatic motif in the cytosolic tail of the mannose receptor mediates endosomal sorting.
    Rohrer J
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29694-700.

    Cysteine34 of the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor is reversibly palmitoylated and required for normal trafficking and lysosomal enzyme sorting.
    Rohrer J
    The Journal of cell biology 132.4 (1996 Feb): 577-84.

    IP References
    Epitope Map References
    All References

    The mannose receptor and the cation-dependent form of mannose 6-phosphate receptor have overlapping cellular and subcellular distributions in liver.
    Messner DJ
    Archives of biochemistry and biophysics 306.2 (1993 Nov 1): 391-401.

    Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins.
    Munro S
    Science (New York, N.Y.) 346.6209 (2014 Oct 31): 1256898.

    A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes.
    Kornfeld S
    The Journal of cell biology 130.6 (1995 Sep): 1297-306.

    Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor.
    Rohrer J
    The Journal of biological chemistry 278.27 (2003 Jul 4): 24753-8.

    The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding.
    Rohrer J
    The Journal of biological chemistry 279.22 (2004 May 28): 23542-9.

    Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells.
    Waguri S
    Archives of histology and cytology 70.5 (2007 Dec): 303-12.

    HPS6 interacts with dynactin p150Glued to mediate retrograde trafficking and maturation of lysosomes.
    Liu JJ
    Journal of cell science 127.Pt 21 (2014 Nov 1): 4574-88.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation.
    Maki RA
    PloS one 11.2 (2016): e0149391.

    Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its cytoplasmic tail.
    Rohrer J
    Proceedings of the National Academy of Sciences of the United States of America 94.26 (1997 Dec 23): 14471-6.

    Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.
    Nixon RA
    The Journal of biological chemistry 277.7 (2002 Feb 15): 5299-307.

    A di-aromatic motif in the cytosolic tail of the mannose receptor mediates endosomal sorting.
    Rohrer J
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29694-700.

    Cysteine34 of the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor is reversibly palmitoylated and required for normal trafficking and lysosomal enzyme sorting.
    Rohrer J
    The Journal of cell biology 132.4 (1996 Feb): 577-84.

    Human cytomegalovirus final envelopment on membranes containing both trans-Golgi network and endosomal markers.
    Fraile-Ramos A
    Cellular microbiology 12.3 (2010 Mar): 386-404.

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