ABL-93
Catalog Fields
Antigen: LAMP-2
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes:
Predicted Species Reactivity:
Gene: Lamp2
Immunogen: BALB/c 3T3 mouse embryo fibroblast tissue culture cell glycoproteins purified by lectin chromatography
Alternate Gene Names: CD107b, Mac3, LGP-B, Igp110
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63-Ag8-653
Epitope Location or Sequence: Luminal region of LAMP-2
Uniprot ID: P17047
Immunogen Sequence: Full length protein
Entrez Gene ID: 16784
Antibody Registry ID: AB_2134767
Additional Information: LAMP2 is a marker of lysosmes and late endosomes. ABL-93 is directed against the luminal domain of LAMP-2 common to all isoforms. RRID: AB_2134767
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ABL-93 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product ABL-93)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
22 References
Initial Publication
Identification of two lysosomal membrane glycoproteins.
August JT
The Journal of cell biology 101.1 (1985 Jul): 85-95.