ABL-93

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$40.00
SKU: ABL-93
View product citations for antibody ABL-93 on CiteAb

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Available: 299

DSHB Data Sheet

Catalog Fields

Antigen: LAMP-2
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes:
Antigen Molecular Weight: Predicted: 43 kDa; Apparent: around 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lamp2
Immunogen: BALB/c 3T3 mouse embryo fibroblast tissue culture cell glycoproteins purified by lectin chromatography
Alternate Gene Names: CD107b, Mac3, LGP-B, Igp110
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63-Ag8-653
Epitope Location or Sequence: Luminal region of LAMP-2
Uniprot ID: P17047 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16784 
Additional Characterization:
Antibody Registry ID: AB_2134767 
Additional Information: LAMP2 is a marker of lysosmes and late endosomes. ABL-93 is directed against the luminal domain of LAMP-2 common to all isoforms. RRID: AB_2134767
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ABL-93 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product ABL-93)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of cell biology
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    IF References

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Willingham MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33.8 (1985 Aug): 813-20.

    The Journal of cell biology
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Brain research
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    The Journal of biological chemistry
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    The Journal of biological chemistry
    Ladisch S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21121-7.

    Proceedings of the National Academy of Sciences of the United States of America
    Kerppola TK
    Proceedings of the National Academy of Sciences of the United States of America 101.41 (2004 Oct 12): 14782-7.

    The Journal of comparative neurology
    Wilkinson RS
    The Journal of comparative neurology 520.18 (2012 Dec 15): 4275-93.

    Molecular reproduction and development
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    The Journal of biological chemistry
    Weisz OA
    The Journal of biological chemistry 281.18 (2006 May 5): 12751-9.

    Traffic (Copenhagen, Denmark)
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    WB References

    Human molecular genetics
    Meisler MH
    Human molecular genetics 18.24 (2009 Dec 15): 4868-78.

    The Journal of biological chemistry
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    The Journal of biological chemistry
    Lotan R
    The Journal of biological chemistry 265.31 (1990 Nov 5): 19192-8.

    Molecular reproduction and development
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Nature
    Saftig P
    Nature 406.6798 (2000 Aug 24): 902-6.

    Traffic (Copenhagen, Denmark)
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    IHC References

    Differentiation; research in biological diversity
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    IP References

    The Journal of cell biology
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    The Journal of biological chemistry
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
    von Figura K
    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 31.2-3 (1999 Feb-Mar): 235-41.

    FACS References

    Journal of immunological methods
    Harding CV
    Journal of immunological methods 213.1 (1998 Apr 1): 53-71.

    Epitope Map References

    Differentiation; research in biological diversity
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    All References

    Differentiation; research in biological diversity
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Willingham MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33.8 (1985 Aug): 813-20.

    The Journal of cell biology
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Brain research
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    The Journal of biological chemistry
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    The Journal of biological chemistry
    Ladisch S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21121-7.

    Proceedings of the National Academy of Sciences of the United States of America
    Kerppola TK
    Proceedings of the National Academy of Sciences of the United States of America 101.41 (2004 Oct 12): 14782-7.

    The Journal of comparative neurology
    Wilkinson RS
    The Journal of comparative neurology 520.18 (2012 Dec 15): 4275-93.

    Molecular reproduction and development
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    The Journal of biological chemistry
    Weisz OA
    The Journal of biological chemistry 281.18 (2006 May 5): 12751-9.

    Traffic (Copenhagen, Denmark)
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    Human molecular genetics
    Meisler MH
    Human molecular genetics 18.24 (2009 Dec 15): 4868-78.

    The Journal of biological chemistry
    Lotan R
    The Journal of biological chemistry 265.31 (1990 Nov 5): 19192-8.

    Nature
    Saftig P
    Nature 406.6798 (2000 Aug 24): 902-6.

    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
    von Figura K
    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 31.2-3 (1999 Feb-Mar): 235-41.

    Journal of immunological methods
    Harding CV
    Journal of immunological methods 213.1 (1998 Apr 1): 53-71.

    Biochemical Society symposium
    August JT
    Biochemical Society symposium 51. (1986): 97-112.

    Cancer research
    Brentani RR
    Cancer research 56.7 (1996 Apr 1): 1682-9.

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