ABL-93

(0) No Reviews yet
$40.00
SKU: ABL-93
View product citations for antibody ABL-93 on CiteAb

In Stock

Available: 317

DSHB Data Sheet

Catalog Fields

Antigen: LAMP-2
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes:
Antigen Molecular Weight: Predicted: 43 kDa; Apparent: around 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Lamp2
Immunogen: BALB/c 3T3 mouse embryo fibroblast tissue culture cell glycoproteins purified by lectin chromatography
Alternate Gene Names: CD107b, Mac3, LGP-B, Igp110
Alternate Antibody Name: CD107b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63-Ag8-653
Epitope Location or Sequence: Luminal region of LAMP-2
Uniprot ID: P17047 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16784 
Additional Characterization:
Antibody Registry ID: AB_2134767 
Additional Information: LAMP2 is a marker of lysosmes and late endosomes. ABL-93 is directed against the luminal domain of LAMP-2 common to all isoforms. RRID: AB_2134767
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ABL-93 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product ABL-93)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    IF References

    Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins.
    Willingham MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33.8 (1985 Aug): 813-20.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron.
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Induction of endocytic vesicles by exogenous C(6)-ceramide.
    Ladisch S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21121-7.

    Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes.
    Kerppola TK
    Proceedings of the National Academy of Sciences of the United States of America 101.41 (2004 Oct 12): 14782-7.

    "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals.
    Wilkinson RS
    The Journal of comparative neurology 520.18 (2012 Dec 15): 4275-93.

    Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis.
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Posttranslational cleavage and adaptor protein complex-dependent trafficking of mucolipin-1.
    Weisz OA
    The Journal of biological chemistry 281.18 (2006 May 5): 12751-9.

    Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes.
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    WB References

    Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2.
    Meisler MH
    Human molecular genetics 18.24 (2009 Dec 15): 4868-78.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Modulation of lysosomal-associated membrane glycoproteins during retinoic acid-induced embryonal carcinoma cell differentiation.
    Lotan R
    The Journal of biological chemistry 265.31 (1990 Nov 5): 19192-8.

    Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis.
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.
    Saftig P
    Nature 406.6798 (2000 Aug 24): 902-6.

    Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes.
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    IHC References

    Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    IP References

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Alteration in pancreatic immunoreactivity of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 and in intracellular degradation of IGFBP-3 in fibroblasts of IGF-II receptor/IGF-II-deficient mice.
    von Figura K
    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 31.2-3 (1999 Feb-Mar): 235-41.

    FACS References

    Flow analysis of MHC molecules and other membrane proteins in isolated phagosomes.
    Harding CV
    Journal of immunological methods 213.1 (1998 Apr 1): 53-71.

    Epitope Map References

    Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    All References

    Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins.
    Willingham MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33.8 (1985 Aug): 813-20.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron.
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Induction of endocytic vesicles by exogenous C(6)-ceramide.
    Ladisch S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21121-7.

    Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes.
    Kerppola TK
    Proceedings of the National Academy of Sciences of the United States of America 101.41 (2004 Oct 12): 14782-7.

    "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals.
    Wilkinson RS
    The Journal of comparative neurology 520.18 (2012 Dec 15): 4275-93.

    Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis.
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Posttranslational cleavage and adaptor protein complex-dependent trafficking of mucolipin-1.
    Weisz OA
    The Journal of biological chemistry 281.18 (2006 May 5): 12751-9.

    Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes.
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2.
    Meisler MH
    Human molecular genetics 18.24 (2009 Dec 15): 4868-78.

    Modulation of lysosomal-associated membrane glycoproteins during retinoic acid-induced embryonal carcinoma cell differentiation.
    Lotan R
    The Journal of biological chemistry 265.31 (1990 Nov 5): 19192-8.

    Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.
    Saftig P
    Nature 406.6798 (2000 Aug 24): 902-6.

    Alteration in pancreatic immunoreactivity of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 and in intracellular degradation of IGFBP-3 in fibroblasts of IGF-II receptor/IGF-II-deficient mice.
    von Figura K
    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 31.2-3 (1999 Feb-Mar): 235-41.

    Flow analysis of MHC molecules and other membrane proteins in isolated phagosomes.
    Harding CV
    Journal of immunological methods 213.1 (1998 Apr 1): 53-71.

    Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2.
    August JT
    Biochemical Society symposium 51. (1986): 97-112.

    alpha6beta1-Integrin, a major cell surface carrier of beta1-6-branched oligosaccharides, mediates migration of EJ-ras-transformed fibroblasts on laminin-1 independently of its glycosylation state.
    Brentani RR
    Cancer research 56.7 (1996 Apr 1): 1682-9.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review