ABL-93

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DSHB Data Sheet

Catalog Fields

Product Name/ID: ABL-93
Available to For-Profits: Yes
Alternate Antibody Name: CD107b
Gene Name: Lamp2
Ab Isotype: RIgG2a
Gene Symbol:
Antibody Registry ID: AB_2134767 
Uniprot ID: P17047 
RRID:  
Entrez Gene ID: 16784 
Clonality: Monoclonal
Immunogen: BALB/c 3T3 mouse embryo fibroblast tissue culture cell glycoproteins purified by lectin chromatography
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3x63-Ag8-653
Epitope Mapped: Yes
Antigen Name: LAMP-2
Epitope Location or Sequence: Luminal region of LAMP-2
Alternate Antigen Name:
Deposit Date: 8/1/1986
Antigen Molecular Weight: Predicted: 43 kDa; Apparent: around 110 kDa
Depositor: August, J.T.
Antigen Sequence:
Depositor Institution: Johns Hopkins University School of Medicine
Antigen Species: Mouse
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: LAMP2 is a marker of lysosmes and late endosomes. ABL-93 is directed against the luminal domain of LAMP-2 common to all isoforms. RRID: AB_2134767
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ABL-93 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product ABL-93)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    IF References

    Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins.
    Willingham MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33.8 (1985 Aug): 813-20.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron.
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Induction of endocytic vesicles by exogenous C(6)-ceramide.
    Ladisch S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21121-7.

    Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes.
    Kerppola TK
    Proceedings of the National Academy of Sciences of the United States of America 101.41 (2004 Oct 12): 14782-7.

    "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals.
    Wilkinson RS
    The Journal of comparative neurology 520.18 (2012 Dec 15): 4275-93.

    Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis.
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Posttranslational cleavage and adaptor protein complex-dependent trafficking of mucolipin-1.
    Weisz OA
    The Journal of biological chemistry 281.18 (2006 May 5): 12751-9.

    Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes.
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    LAMP2 deficiency attenuates the neurodegeneration markers induced by HSV-1 infection.
    Aldudo J
    Neurochemistry international 146. (2021 Jun): 105032.

    ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H(+)-ATPases is essential for brain development in humans and mice.
    Saitsu H
    Nature communications 12.1 (2021 Apr 8): 2107.

    Loss of Soluble (Pro)renin Receptor Attenuates Angiotensin-II Induced Hypertension and Renal Injury.
    Kohan DE
    Circulation research 129.1 (2021 Jun 25): 50-62.

    WB References

    Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2.
    Meisler MH
    Human molecular genetics 18.24 (2009 Dec 15): 4868-78.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Modulation of lysosomal-associated membrane glycoproteins during retinoic acid-induced embryonal carcinoma cell differentiation.
    Lotan R
    The Journal of biological chemistry 265.31 (1990 Nov 5): 19192-8.

    Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis.
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.
    Saftig P
    Nature 406.6798 (2000 Aug 24): 902-6.

    Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes.
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    LAMP2 deficiency attenuates the neurodegeneration markers induced by HSV-1 infection.
    Aldudo J
    Neurochemistry international 146. (2021 Jun): 105032.

    IHC References

    Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H(+)-ATPases is essential for brain development in humans and mice.
    Saitsu H
    Nature communications 12.1 (2021 Apr 8): 2107.

    IP References

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Alteration in pancreatic immunoreactivity of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 and in intracellular degradation of IGFBP-3 in fibroblasts of IGF-II receptor/IGF-II-deficient mice.
    von Figura K
    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 31.2-3 (1999 Feb-Mar): 235-41.

    FACS References

    Flow analysis of MHC molecules and other membrane proteins in isolated phagosomes.
    Harding CV
    Journal of immunological methods 213.1 (1998 Apr 1): 53-71.

    Epitope Map References

    Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    All References

    Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.
    Konecki DS
    Differentiation; research in biological diversity 65.1 (1999 Jul): 43-58.

    α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2.
    Dauer WT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5724-42.

    ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H(+)-ATPases is essential for brain development in humans and mice.
    Saitsu H
    Nature communications 12.1 (2021 Apr 8): 2107.

    Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins.
    Willingham MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 33.8 (1985 Aug): 813-20.

    Identification of two lysosomal membrane glycoproteins.
    August JT
    The Journal of cell biology 101.1 (1985 Jul): 85-95.

    Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron.
    Mazur-Kolecka B
    Brain research 1002.1-2 (2004 Mar 26): 67-75.

    Normal lysosomal morphology and function in LAMP-1-deficient mice.
    Saftig P
    The Journal of biological chemistry 274.18 (1999 Apr 30): 12692-701.

    Induction of endocytic vesicles by exogenous C(6)-ceramide.
    Ladisch S
    The Journal of biological chemistry 274.30 (1999 Jul 23): 21121-7.

    Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes.
    Kerppola TK
    Proceedings of the National Academy of Sciences of the United States of America 101.41 (2004 Oct 12): 14782-7.

    "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals.
    Wilkinson RS
    The Journal of comparative neurology 520.18 (2012 Dec 15): 4275-93.

    Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis.
    Moreno RD
    Molecular reproduction and development 66.2 (2003 Oct): 202-9.

    Posttranslational cleavage and adaptor protein complex-dependent trafficking of mucolipin-1.
    Weisz OA
    The Journal of biological chemistry 281.18 (2006 May 5): 12751-9.

    Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes.
    Kreibich G
    Traffic (Copenhagen, Denmark) 10.9 (2009 Sep): 1350-61.

    LAMP2 deficiency attenuates the neurodegeneration markers induced by HSV-1 infection.
    Aldudo J
    Neurochemistry international 146. (2021 Jun): 105032.

    Loss of Soluble (Pro)renin Receptor Attenuates Angiotensin-II Induced Hypertension and Renal Injury.
    Kohan DE
    Circulation research 129.1 (2021 Jun 25): 50-62.

    Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2.
    Meisler MH
    Human molecular genetics 18.24 (2009 Dec 15): 4868-78.

    Modulation of lysosomal-associated membrane glycoproteins during retinoic acid-induced embryonal carcinoma cell differentiation.
    Lotan R
    The Journal of biological chemistry 265.31 (1990 Nov 5): 19192-8.

    Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.
    Saftig P
    Nature 406.6798 (2000 Aug 24): 902-6.

    Alteration in pancreatic immunoreactivity of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 and in intracellular degradation of IGFBP-3 in fibroblasts of IGF-II receptor/IGF-II-deficient mice.
    von Figura K
    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 31.2-3 (1999 Feb-Mar): 235-41.

    Flow analysis of MHC molecules and other membrane proteins in isolated phagosomes.
    Harding CV
    Journal of immunological methods 213.1 (1998 Apr 1): 53-71.

    Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2.
    August JT
    Biochemical Society symposium 51. (1986): 97-112.

    alpha6beta1-Integrin, a major cell surface carrier of beta1-6-branched oligosaccharides, mediates migration of EJ-ras-transformed fibroblasts on laminin-1 independently of its glycosylation state.
    Brentani RR
    Cancer research 56.7 (1996 Apr 1): 1682-9.

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