DSHB-GFP-2G6

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SKU: DSHB-GFP-2G6-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: DSHB-GFP-2G6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: GFP
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2617421 
Uniprot ID: P42218 
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Recombinant protein
Clone:
Immunogen Sequence: Full length
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Antigen Name: Green fluorescent protein (GFP)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/15/2013
Antigen Molecular Weight: 26.8 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Aequorea victoria
Depositor Notes: DSHB-GFP-2G6 does not western blot.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Aequorea victoria, All species for fusion proteins
Additional Information: RRID:AB_2617421
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-2G6 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-2G6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • IP References
  • ChIP References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication
    IF References
    IP References
    ChIP References
    FFPE References
    FACS References
    All References

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