DSHB-GFP-1G9
Catalog Fields
Antigen: Green fluorescent protein (GFP)
Hybridoma Cells Available: No
Antigen Species: Aequorea victoria
Depositor: DSHB
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Aequorea victoria, All species for fusion proteins
Depositors Notes: DSHB-GFP-1G9 does not western blot.
Predicted Species Reactivity:
Gene: GFP
Immunogen: Recombinant protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: P42212
Immunogen Sequence: Full length
Antibody Registry ID: AB_2617420
Additional Information: 1G9 also recognizes eGFP, BFP, and CFP but not mCherry or RFP as determined by ELISA.. RRID:AB_2617420
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-1G9 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-1G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Generating a battery of monoclonal antibodies against native green fluorescent protein for immunostaining, FACS, IP, and ChIP using a unique adjuvant.
Soll DR
Monoclonal antibodies in immunodiagnosis and immunotherapy 33.2 (2014 Apr): 80-8.