DSHB-GFP-1G9
Catalog Fields
Antigen: Green fluorescent protein (GFP)
Hybridoma Cells Available: No
Antigen Species: Aequorea victoria
Depositor: DSHB
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: DSHB-GFP-1G9 does not western blot.
Positive Tested Species Reactivity: Aequorea victoria, All species for fusion proteins
Gene: GFP
Antigen Molecular Weight: 26.8 kDa
Clonality: Monoclonal
Immunogen: Recombinant protein
Epitope Mapped: No
Myeloma Strain: Sp2/0-Ag14
Immunogen Sequence: Full length
Uniprot ID: P42212
Additional Information: 1G9 also recognizes eGFP, BFP, and CFP but not mCherry or RFP as determined by ELISA.. RRID:AB_2617420
Antibody Registry ID: AB_2617420
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP-1G9 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP-1G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Generating a battery of monoclonal antibodies against native green fluorescent protein for immunostaining, FACS, IP, and ChIP using a unique adjuvant.
Soll DR
Monoclonal antibodies in immunodiagnosis and immunotherapy 33.2 (2014 Apr): 80-8.