Antigen: Green fluorescent protein (GFP) variant
Hybridoma Cells Available: Yes
Antigen Species: Aequorea victoria
Depositor: Sanes, J.R. / Yamagata, M.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Harvard University
Positive Tested Species Reactivity: Aequorea victoria, All species for fusion proteins
Depositors Notes: GFP-G1 does not western blot.
Antigen Molecular Weight: 26.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant protein
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: FOX-NY
Epitope Location or Sequence: GFP1-10 fragment
Uniprot ID: P42212
Immunogen Sequence: Full length
Entrez Gene ID:
Antibody Registry ID: AB_2619561
Additional Information: GFP-G1 recognizes all GFP derivatives tested (EGFP, Venus, EYFP, ECFP/cerulean) including the GFP1-10 fragment. Paraformaldehyde (4%) or methanol fixation is recommended. RRID:AB_2619561
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GFP-G1 was deposited to the DSHB by Sanes, J.R. / Yamagata, M. (DSHB Hybridoma Product GFP-G1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Nature communications 4. (2013): 2790.
Epitope Map References