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View product citations for antibody GFP-G1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Green fluorescent protein (GFP) variant
Hybridoma Cells Available: Yes
Antigen Species: Aequorea victoria
Depositor: Sanes, J.R. / Yamagata, M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard University
Positive Tested Species Reactivity: Aequorea victoria, All species for fusion proteins
Depositors Notes: GFP-G1 does not western blot.
Antigen Molecular Weight: 26.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: GFP
Immunogen: Recombinant protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: FOX-NY
Epitope Location or Sequence: GFP1-10 fragment
Uniprot ID: P42212 
Immunogen Sequence: Full length
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2619561 
Additional Information: GFP-G1 recognizes all GFP derivatives tested (EGFP, Venus, EYFP, ECFP/cerulean) including the GFP1-10 fragment. Paraformaldehyde (4%) or methanol fixation is recommended. RRID:AB_2619561
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GFP-G1 was deposited to the DSHB by Sanes, J.R. / Yamagata, M. (DSHB Hybridoma Product GFP-G1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
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