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View product citations for antibody HERMES-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD44 (all isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Butcher, E.C.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Human, Zebrafish
Depositors Notes: The HERMES-1 antibody recognizes the N-terminal hyaluronate binding domain of CD44.
Antigen Molecular Weight: 81.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CD44
Immunogen: Tonsil lymphocytes
Alternate Gene Names: CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: Epitope cluster I
Uniprot ID: P16070 
Immunogen Sequence: Full length protein
Entrez Gene ID: 960 
Additional Characterization:  
Antibody Registry ID: AB_528148 
Additional Information: RRID:AB_528148
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HERMES-1 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product HERMES-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man.
    Butcher EC
    European journal of immunology 16.10 (1986 Oct): 1195-202.

    Distribution and function of tenascin during cranial neural crest development in the chick.
    Bronner-Fraser M
    Journal of neuroscience research 21.2-4 (1988 Oct-Dec): 135-47.

    IP References
    FB References
    FACS References
    Epitope Map References
    All References

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