HERMES-1

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$45.00 to $425.00
SKU: HERMES-1
View product citations for antibody HERMES-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD44 (all isoforms)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG2a
Depositor: Butcher, E.C.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Human, Zebrafish
Depositors Institution: Stanford University School of Medicine
Antigen Molecular Weight: 81.5 kDa
Depositors Notes: The HERMES-1 antibody recognizes the N-terminal hyaluronate binding domain of CD44.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Tonsil lymphocytes
Gene: CD44
Alternate Antibody Name:
Alternate Gene Names: CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P16070 
Epitope Location or Sequence: Epitope cluster I
Entrez Gene ID: 960 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528148 
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: RRID:AB_528148
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HERMES-1 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product HERMES-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man.
    Butcher EC
    European journal of immunology 16.10 (1986 Oct): 1195-202.

    Distribution and function of tenascin during cranial neural crest development in the chick.
    Bronner-Fraser M
    Journal of neuroscience research 21.2-4 (1988 Oct-Dec): 135-47.

    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man.
    Butcher EC
    European journal of immunology 16.10 (1986 Oct): 1195-202.

    Distribution and function of tenascin during cranial neural crest development in the chick.
    Bronner-Fraser M
    Journal of neuroscience research 21.2-4 (1988 Oct-Dec): 135-47.

    Monoclonal antibodies against the CD44 [In(Lu)-related p80], and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors.
    Butcher EC
    Journal of immunology (Baltimore, Md. : 1950) 142.6 (1989 Mar 15): 2046-51.

    Acute Myeloid and Lymphoblastic Leukemia Cell Interactions with Endothelial Selectins: Critical Role of PSGL-1, CD44 and CD43.
    Spertini O
    Cancers 11.9 (2019 Aug 27): .

    Biomolecular characterization of CD44-fibrin(ogen) binding: distinct molecular requirements mediate binding of standard and variant isoforms of CD44 to immobilized fibrin(ogen).
    Konstantopoulos K
    The Journal of biological chemistry 284.2 (2009 Jan 9): 1177-89.

    P16INK4a is required for hSNF5 chromatin remodeler-induced cellular senescence in malignant rhabdoid tumor cells.
    Verrijzer CP
    The Journal of biological chemistry 279.5 (2004 Jan 30): 3807-16.

    A human lymphocyte homing receptor, the hermes antigen, is related to cartilage proteoglycan core and link proteins.
    Butcher EC
    Cell 56.6 (1989 Mar 24): 1063-72.

    The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins.
    Underhill C
    The Journal of cell biology 111.6 Pt 1 (1990 Dec): 2765-74.

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