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View product citations for antibody HERMES-1 on CiteAb

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Available: 58

DSHB Data Sheet

Catalog Fields

Antigen: CD44 (all isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Butcher, E.C.
Isotype: RIgG2a
Depositors Institution: Stanford University School of Medicine
Host Species: rat
Depositors Notes: The HERMES-1 antibody recognizes the N-terminal hyaluronate binding domain of CD44.
Positive Tested Species Reactivity: Human, Zebrafish
Gene: CD44
Antigen Molecular Weight: 81.5 kDa
Alternate Gene Names: CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Immunogen: Tonsil lymphocytes
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P16070 
Epitope Location or Sequence: Epitope cluster I
Entrez Gene ID: 960 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528148 
Additional Information: RRID:AB_528148
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HERMES-1 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product HERMES-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man.
    Butcher EC
    European journal of immunology 16.10 (1986 Oct): 1195-202.

    Distribution and function of tenascin during cranial neural crest development in the chick.
    Bronner-Fraser M
    Journal of neuroscience research 21.2-4 (1988 Oct-Dec): 135-47.

    IP References
    FB References
    FACS References
    Epitope Map References
    All References

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