H4C4

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$40.00
SKU: H4C4
View product citations for antibody H4C4 on CiteAb

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Available: 131

DSHB Data Sheet

Catalog Fields

Antigen: CD44, hyaluronate receptor
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: August, J.T. / Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes:
Antigen Molecular Weight: 81.5 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CD44
Immunogen: Adherent monocytes and macrophages from the spleen
Alternate Gene Names: CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence: Extracellular domain a.a. 69-90
Uniprot ID: P16070 
Immunogen Sequence: Full length protein
Entrez Gene ID: 960 
Additional Characterization:
Antibody Registry ID: AB_528147 
Additional Information: RRID:AB_528147
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H4C4 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H4C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    IF References

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    Lipooligosaccharide-independent alteration of cellular homeostasis in Neisseria meningitidis-infected epithelial cells.
    Muckenthaler MU
    Cellular microbiology 7.6 (2005 Jun): 869-85.

    COG complex-mediated recycling of Golgi glycosyltransferases is essential for normal protein glycosylation.
    Lupashin V
    Traffic (Copenhagen, Denmark) 7.2 (2006 Feb): 191-204.

    Functional effects of adult human olfactory stem cells on early-onset sensorineural hearing loss.
    Oleskevich S
    Stem cells (Dayton, Ohio) 29.4 (2011 Apr): 670-7.

    Response of Chondrocytes to Local Mechanical Injury in an Ex Vivo Model.
    Lee GM
    Cartilage 3.1 (2012 Jan): 58-69.

    HAb18G/CD147 promotes pSTAT3-mediated pancreatic cancer development via CD44s.
    Xu L
    Clinical cancer research : an official journal of the American Association for Cancer Research 19.24 (2013 Dec 15): 6703-15.

    Antibody against CD44s inhibits pancreatic tumor initiation and postradiation recurrence in mice.
    Xu L
    Gastroenterology 146.4 (2014 Apr): 1108-18.

    Hyaluronic acid enhances the mechanical properties of tissue-engineered cartilage constructs.
    Klein TJ
    PloS one 9.12 (2014): e113216.

    Melanoma cells undergo aggressive coalescence in a 3D Matrigel model that is repressed by anti-CD44.
    Soll DR
    PloS one 12.3 (2017): e0173400.

    Extensive CD44-dependent hyaluronan coats on human bone marrow-derived mesenchymal stem cells produced by hyaluronan synthases HAS1, HAS2 and HAS3.
    Lammi MJ
    The international journal of biochemistry and cell biology 48. (2014 Mar): 45-54.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    WB References

    P16INK4a is required for hSNF5 chromatin remodeler-induced cellular senescence in malignant rhabdoid tumor cells.
    Verrijzer CP
    The Journal of biological chemistry 279.5 (2004 Jan 30): 3807-16.

    COG complex-mediated recycling of Golgi glycosyltransferases is essential for normal protein glycosylation.
    Lupashin V
    Traffic (Copenhagen, Denmark) 7.2 (2006 Feb): 191-204.

    Soluble CD44 interacts with intermediate filament protein vimentin on endothelial cell surface.
    Kogerman P
    PloS one 6.12 (2011): e29305.

    HAb18G/CD147 promotes pSTAT3-mediated pancreatic cancer development via CD44s.
    Xu L
    Clinical cancer research : an official journal of the American Association for Cancer Research 19.24 (2013 Dec 15): 6703-15.

    Expression of CD44 in human lung tumors.
    Rosada C
    Cancer research 54.5 (1994 Mar 1): 1381-7.

    Hyaluronidase and CD44 hyaluronan receptor expression in squamous cell laryngeal carcinoma.
    Vynios DH
    Biochimica et biophysica acta 1760.7 (2006 Jul): 1039-45.

    IHC References

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    A tissue-engineered humanized xenograft model of human breast cancer metastasis to bone.
    Hutmacher DW
    Disease models and mechanisms 7.2 (2014 Feb): 299-309.

    Antibody against CD44s inhibits pancreatic tumor initiation and postradiation recurrence in mice.
    Xu L
    Gastroenterology 146.4 (2014 Apr): 1108-18.

    The immunohistochemical profile of the tethered filum terminale.
    Cummings TJ
    Pediatric neurosurgery 39.5 (2003 Nov): 227-33.

    Expression of CD44 in human lung tumors.
    Rosada C
    Cancer research 54.5 (1994 Mar 1): 1381-7.

    High CD44 expression on human mesotheliomas mediates association with hyaluronan.
    Pass HI
    The cancer journal from Scientific American 1.3 (1995 Sep-Oct): 196-203.

    CD44 and CD44v6 downregulation in clinical prostatic carcinoma: relation to Gleason grade and cytoarchitecture.
    Miller GJ
    The Prostate 34.3 (1998 Feb 15): 162-8.

    Hyaluronidase and CD44 hyaluronan receptor expression in squamous cell laryngeal carcinoma.
    Vynios DH
    Biochimica et biophysica acta 1760.7 (2006 Jul): 1039-45.

    IP References

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    P16INK4a is required for hSNF5 chromatin remodeler-induced cellular senescence in malignant rhabdoid tumor cells.
    Verrijzer CP
    The Journal of biological chemistry 279.5 (2004 Jan 30): 3807-16.

    Soluble CD44 interacts with intermediate filament protein vimentin on endothelial cell surface.
    Kogerman P
    PloS one 6.12 (2011): e29305.

    HAb18G/CD147 promotes pSTAT3-mediated pancreatic cancer development via CD44s.
    Xu L
    Clinical cancer research : an official journal of the American Association for Cancer Research 19.24 (2013 Dec 15): 6703-15.

    HIV-induced loss of CD44 expression in monocytic cell lines.
    Hildreth JE
    Journal of immunology (Baltimore, Md. : 1950) 151.4 (1993 Aug 15): 2225-36.

    ELISA References

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    HIV-induced loss of CD44 expression in monocytic cell lines.
    Hildreth JE
    Journal of immunology (Baltimore, Md. : 1950) 151.4 (1993 Aug 15): 2225-36.

    FFPE References

    Expression of CD44 in human lung tumors.
    Rosada C
    Cancer research 54.5 (1994 Mar 1): 1381-7.

    FB References

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    HAb18G/CD147 promotes pSTAT3-mediated pancreatic cancer development via CD44s.
    Xu L
    Clinical cancer research : an official journal of the American Association for Cancer Research 19.24 (2013 Dec 15): 6703-15.

    Antibody against CD44s inhibits pancreatic tumor initiation and postradiation recurrence in mice.
    Xu L
    Gastroenterology 146.4 (2014 Apr): 1108-18.

    Melanoma cells undergo aggressive coalescence in a 3D Matrigel model that is repressed by anti-CD44.
    Soll DR
    PloS one 12.3 (2017): e0173400.

    Human ovarian tumour cells can bind hyaluronic acid via membrane CD44: a possible step in peritoneal metastasis.
    Turner GA
    Clinical and experimental metastasis 14.4 (1996 Sep): 325-34.

    High CD44 expression on human mesotheliomas mediates association with hyaluronan.
    Pass HI
    The cancer journal from Scientific American 1.3 (1995 Sep-Oct): 196-203.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    FACS References

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Antibody against CD44s inhibits pancreatic tumor initiation and postradiation recurrence in mice.
    Xu L
    Gastroenterology 146.4 (2014 Apr): 1108-18.

    Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions.
    Varoga DJ
    BMC musculoskeletal disorders 16. (2015 Sep 12): 250.

    HIV-induced loss of CD44 expression in monocytic cell lines.
    Hildreth JE
    Journal of immunology (Baltimore, Md. : 1950) 151.4 (1993 Aug 15): 2225-36.

    Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44.
    Noble PW
    The Journal of clinical investigation 98.10 (1996 Nov 15): 2403-13.

    Expression of CD44 in human lung tumors.
    Rosada C
    Cancer research 54.5 (1994 Mar 1): 1381-7.

    Epitope Map References

    Redistribution of a major cell surface glycoprotein during cell movement.
    August JT
    The Journal of cell biology 99.5 (1984 Nov): 1613-23.

    All References

    Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic-like stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 277.1 (2004 Mar): 178-203.

    A tissue-engineered humanized xenograft model of human breast cancer metastasis to bone.
    Hutmacher DW
    Disease models and mechanisms 7.2 (2014 Feb): 299-309.

    Antibody against CD44s inhibits pancreatic tumor initiation and postradiation recurrence in mice.
    Xu L
    Gastroenterology 146.4 (2014 Apr): 1108-18.

    The immunohistochemical profile of the tethered filum terminale.
    Cummings TJ
    Pediatric neurosurgery 39.5 (2003 Nov): 227-33.

    Expression of CD44 in human lung tumors.
    Rosada C
    Cancer research 54.5 (1994 Mar 1): 1381-7.

    High CD44 expression on human mesotheliomas mediates association with hyaluronan.
    Pass HI
    The cancer journal from Scientific American 1.3 (1995 Sep-Oct): 196-203.

    CD44 and CD44v6 downregulation in clinical prostatic carcinoma: relation to Gleason grade and cytoarchitecture.
    Miller GJ
    The Prostate 34.3 (1998 Feb 15): 162-8.

    Hyaluronidase and CD44 hyaluronan receptor expression in squamous cell laryngeal carcinoma.
    Vynios DH
    Biochimica et biophysica acta 1760.7 (2006 Jul): 1039-45.

    Homotypic cell aggregation induced by anti-CD44(Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 144.5 (1990 Mar 1): 1661-70.

    Lipooligosaccharide-independent alteration of cellular homeostasis in Neisseria meningitidis-infected epithelial cells.
    Muckenthaler MU
    Cellular microbiology 7.6 (2005 Jun): 869-85.

    COG complex-mediated recycling of Golgi glycosyltransferases is essential for normal protein glycosylation.
    Lupashin V
    Traffic (Copenhagen, Denmark) 7.2 (2006 Feb): 191-204.

    Functional effects of adult human olfactory stem cells on early-onset sensorineural hearing loss.
    Oleskevich S
    Stem cells (Dayton, Ohio) 29.4 (2011 Apr): 670-7.

    Response of Chondrocytes to Local Mechanical Injury in an Ex Vivo Model.
    Lee GM
    Cartilage 3.1 (2012 Jan): 58-69.

    HAb18G/CD147 promotes pSTAT3-mediated pancreatic cancer development via CD44s.
    Xu L
    Clinical cancer research : an official journal of the American Association for Cancer Research 19.24 (2013 Dec 15): 6703-15.

    Hyaluronic acid enhances the mechanical properties of tissue-engineered cartilage constructs.
    Klein TJ
    PloS one 9.12 (2014): e113216.

    Melanoma cells undergo aggressive coalescence in a 3D Matrigel model that is repressed by anti-CD44.
    Soll DR
    PloS one 12.3 (2017): e0173400.

    Extensive CD44-dependent hyaluronan coats on human bone marrow-derived mesenchymal stem cells produced by hyaluronan synthases HAS1, HAS2 and HAS3.
    Lammi MJ
    The international journal of biochemistry and cell biology 48. (2014 Mar): 45-54.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    P16INK4a is required for hSNF5 chromatin remodeler-induced cellular senescence in malignant rhabdoid tumor cells.
    Verrijzer CP
    The Journal of biological chemistry 279.5 (2004 Jan 30): 3807-16.

    Soluble CD44 interacts with intermediate filament protein vimentin on endothelial cell surface.
    Kogerman P
    PloS one 6.12 (2011): e29305.

    HIV-induced loss of CD44 expression in monocytic cell lines.
    Hildreth JE
    Journal of immunology (Baltimore, Md. : 1950) 151.4 (1993 Aug 15): 2225-36.

    Human ovarian tumour cells can bind hyaluronic acid via membrane CD44: a possible step in peritoneal metastasis.
    Turner GA
    Clinical and experimental metastasis 14.4 (1996 Sep): 325-34.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Abrasion arthroplasty increases mesenchymal stem cell content of postoperative joint effusions.
    Varoga DJ
    BMC musculoskeletal disorders 16. (2015 Sep 12): 250.

    Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44.
    Noble PW
    The Journal of clinical investigation 98.10 (1996 Nov 15): 2403-13.

    Redistribution of a major cell surface glycoprotein during cell movement.
    August JT
    The Journal of cell biology 99.5 (1984 Nov): 1613-23.

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