TI-4

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$40.00
SKU: TI-4
View product citations for antibody TI-4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: troponin I, cardiac and slow-twitch skeletal
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padova,Padova, Italy
Positive Tested Species Reactivity: Canine, Goat, Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: 24.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TNNI3
Immunogen: Purified troponin complex/adult bovine heart
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: P08057 
Immunogen Sequence:
Entrez Gene ID: 511094 
Additional Characterization:  
Antibody Registry ID: AB_10573815 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TI-4 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product TI-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Troponin I switching in the developing heart.
    Schiaffino S
    The Journal of biological chemistry 264.27 (1989 Sep 25): 16299-302.

    IF References

    Gold nanorod-incorporated gelatin-based conductive hydrogels for engineering cardiac tissue constructs.
    Nikkhah M
    Acta biomaterialia 41. (2016 Sep 1): 133-46.

    3D cardiac microtissues encapsulated with the co-culture of cardiomyocytes and cardiac fibroblasts.
    Nikkhah M
    Advanced healthcare materials 4.13 (2015 Sep 16): 1961-71.

    PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering.
    Nikkhah M
    Acta biomaterialia 32. (2016 Mar 1): 10-23.

    Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function.
    Metzger JM
    Proceedings of the National Academy of Sciences of the United States of America 94.10 (1997 May 13): 5444-9.

    Wireless Passive Stimulation of Engineered Cardiac Tissues.
    Chae J
    ACS sensors 2.7 (2017 Jul 28): 1006-1012.

    Troponin isoform switching in the developing heart and its functional consequences.
    Ausoni S
    Trends in cardiovascular medicine 3.1 (1993 Jan-Feb): 12-7.

    Troponin I isoform expression is developmentally regulated in differentiating embryonic stem cell-derived cardiac myocytes.
    Metzger JM
    Developmental dynamics : an official publication of the American Association of Anatomists 206.1 (1996 May): 24-38.

    Regional differences in troponin I isoform switching during rat heart development.
    Schiaffino S
    Developmental biology 156.1 (1993 Mar): 253-64.

    Functional analysis of troponin I regulatory domains in the intact myofilament of adult single cardiac myocytes.
    Metzger JM
    The Journal of biological chemistry 274.32 (1999 Aug 6): 22508-16.

    Development of Bipotent Cardiac/Skeletal Myogenic Progenitors from MESP1+ Mesoderm.
    Kyba M
    Stem cell reports 6.1 (2016 Jan 12): 26-34.

    WB References

    Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers.
    Bang ML
    Cardiovascular research 107.2 (2015 Jul 15): 216-25.

    Complex tropomyosin and troponin T isoform expression patterns in orbital and global fibers of adult dog and rat extraocular muscles.
    Reiser PJ
    Journal of muscle research and cell motility 34.3-4 (2013 Aug): 211-31.

    Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function.
    Metzger JM
    Proceedings of the National Academy of Sciences of the United States of America 94.10 (1997 May 13): 5444-9.

    Troponin I isoform expression is developmentally regulated in differentiating embryonic stem cell-derived cardiac myocytes.
    Metzger JM
    Developmental dynamics : an official publication of the American Association of Anatomists 206.1 (1996 May): 24-38.

    Regional differences in troponin I isoform switching during rat heart development.
    Schiaffino S
    Developmental biology 156.1 (1993 Mar): 253-64.

    Functional analysis of troponin I regulatory domains in the intact myofilament of adult single cardiac myocytes.
    Metzger JM
    The Journal of biological chemistry 274.32 (1999 Aug 6): 22508-16.

    Troponin I isoform expression in human and experimental atrial fibrillation.
    van Eys GJ
    Circulation 110.7 (2004 Aug 17): 770-5.

    Development of Bipotent Cardiac/Skeletal Myogenic Progenitors from MESP1+ Mesoderm.
    Kyba M
    Stem cell reports 6.1 (2016 Jan 12): 26-34.

    Ectopic expression of Cdk8 induces eccentric hypertrophy and heart failure.
    Grueter CE
    JCI insight 2.15 (2017 Aug 3): .

    IHC References

    Functional analysis of troponin I regulatory domains in the intact myofilament of adult single cardiac myocytes.
    Metzger JM
    The Journal of biological chemistry 274.32 (1999 Aug 6): 22508-16.

    Regional differences in troponin I isoform switching during rat heart development.
    Schiaffino S
    Developmental biology 156.1 (1993 Mar): 253-64.

    All References

    Functional analysis of troponin I regulatory domains in the intact myofilament of adult single cardiac myocytes.
    Metzger JM
    The Journal of biological chemistry 274.32 (1999 Aug 6): 22508-16.

    Regional differences in troponin I isoform switching during rat heart development.
    Schiaffino S
    Developmental biology 156.1 (1993 Mar): 253-64.

    Gold nanorod-incorporated gelatin-based conductive hydrogels for engineering cardiac tissue constructs.
    Nikkhah M
    Acta biomaterialia 41. (2016 Sep 1): 133-46.

    3D cardiac microtissues encapsulated with the co-culture of cardiomyocytes and cardiac fibroblasts.
    Nikkhah M
    Advanced healthcare materials 4.13 (2015 Sep 16): 1961-71.

    PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering.
    Nikkhah M
    Acta biomaterialia 32. (2016 Mar 1): 10-23.

    Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function.
    Metzger JM
    Proceedings of the National Academy of Sciences of the United States of America 94.10 (1997 May 13): 5444-9.

    Wireless Passive Stimulation of Engineered Cardiac Tissues.
    Chae J
    ACS sensors 2.7 (2017 Jul 28): 1006-1012.

    Troponin isoform switching in the developing heart and its functional consequences.
    Ausoni S
    Trends in cardiovascular medicine 3.1 (1993 Jan-Feb): 12-7.

    Troponin I isoform expression is developmentally regulated in differentiating embryonic stem cell-derived cardiac myocytes.
    Metzger JM
    Developmental dynamics : an official publication of the American Association of Anatomists 206.1 (1996 May): 24-38.

    Development of Bipotent Cardiac/Skeletal Myogenic Progenitors from MESP1+ Mesoderm.
    Kyba M
    Stem cell reports 6.1 (2016 Jan 12): 26-34.

    Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers.
    Bang ML
    Cardiovascular research 107.2 (2015 Jul 15): 216-25.

    Complex tropomyosin and troponin T isoform expression patterns in orbital and global fibers of adult dog and rat extraocular muscles.
    Reiser PJ
    Journal of muscle research and cell motility 34.3-4 (2013 Aug): 211-31.

    Troponin I isoform expression in human and experimental atrial fibrillation.
    van Eys GJ
    Circulation 110.7 (2004 Aug 17): 770-5.

    Ectopic expression of Cdk8 induces eccentric hypertrophy and heart failure.
    Grueter CE
    JCI insight 2.15 (2017 Aug 3): .

    Troponin I switching in the developing heart.
    Schiaffino S
    The Journal of biological chemistry 264.27 (1989 Sep 25): 16299-302.

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