RV-C2
Catalog Fields
Product Name/ID: RV-C2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: TNNT2
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2240831
Uniprot ID: P09741
Entrez Gene ID: 100009428
Clonality: Monoclonal
Immunogen: Myofibrillar preparation from ventricle
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: NS0
Epitope Mapped: No
Antigen Name: Troponin T, cardiac type
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/15/2009
Antigen Molecular Weight: 35.8 kDa
Depositor: Schiaffino, S.
Antigen Sequence:
Depositor Institution: Universita degli Studi di Padova
Antigen Species: Rabbit
Depositor Notes: This antibody immunostains fetal, neonatal and adult cardiac fibers, and some fetal and neonatal skeletal muscle fibers. The epitope is not phosphorylation specific.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Human, Mouse, Rat
Additional Information: RRID: AB_2240831
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RV-C2 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product RV-C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Troponin T switching in the developing rat heart.
Schiaffino S
The Journal of biological chemistry 263.34 (1988 Dec 5): 18488-92.