RV-C2

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$40.00
SKU: RV-C2
View product citations for antibody RV-C2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Troponin T2, cardiac type
Hybridoma Cells Available: Yes
Antigen Species: Rabbit
Depositor: Schiaffino, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padova
Positive Tested Species Reactivity: Bovine, Human, Mouse, Rat
Depositors Notes: This antibody immunostains fetal, neonatal and adult cardiac fibers, and some fetal and neonatal skeletal muscle fibers. The epitope is not phosphorylation specific.
Antigen Molecular Weight: 35.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TNNT2
Immunogen: Myofibrillar preparation from ventricle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: P09741 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 100009428 
Additional Characterization:  
Antibody Registry ID: AB_2240831 
Additional Information: RRID: AB_2240831
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RV-C2 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product RV-C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Troponin T switching in the developing rat heart.
    Schiaffino S
    The Journal of biological chemistry 263.34 (1988 Dec 5): 18488-92.

    IF References

    Transplantation of Adipose-Derived Stem Cell Sheet Attenuates Adverse Cardiac Remodeling in Acute Myocardial Infarction.
    Lim DS
    Tissue engineering. Part A 23.1-2 (2017 Jan): 1-11.

    Troponin T switching in the developing rat heart.
    Schiaffino S
    The Journal of biological chemistry 263.34 (1988 Dec 5): 18488-92.

    Cardiac troponin T in developing, regenerating and denervated rat skeletal muscle.
    Schiaffino S
    Development (Cambridge, England) 110.2 (1990 Oct): 547-54.

    HSPB7 is indispensable for heart development by modulating actin filament assembly.
    Chen J
    Proceedings of the National Academy of Sciences of the United States of America 114.45 (2017 Nov 7): 11956-11961.

    Transplantation of Immortalized CD34+ and CD34- Adipose-Derived Stem Cells Improve Cardiac Function and Mitigate Systemic Pro-Inflammatory Responses.
    Lim DS
    PloS one 11.2 (2016): e0147853.

    Biomimetic Cardiac Tissue Model Enables the Adaption of Human Induced Pluripotent Stem Cell Cardiomyocytes to Physiological Hemodynamic Loads.
    Sethu P
    Analytical chemistry 88.19 (2016 Oct 4): 9862-9868.

    Troponin T- and troponin I-like proteins in bovine vascular smooth muscle.
    Sartore S
    Circulation research 68.5 (1991 May): 1349-61.

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement.
    Lim DS
    ACS applied materials & interfaces 9.20 (2017 May 24): 16803-16812.

    Isolation of contractile cardiomyocytes from human pluripotent stem-cell-derived cardiomyogenic cultures using a human NCX1-EGFP reporter.
    Wolvetang EJ
    Stem cells and development 24.1 (2015 Jan 1): 11-20.

    Preventing permeability transition pore opening increases mitochondrial maturation, myocyte differentiation and cardiac function in the neonatal mouse heart.
    Porter GA Jr
    Pediatric research 81.6 (2017 Jun): 932-941.

    Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction.
    Lim DS
    PloS one 11.6 (2016): e0158067.

    WB References

    Troponin T switching in the developing rat heart.
    Schiaffino S
    The Journal of biological chemistry 263.34 (1988 Dec 5): 18488-92.

    Troponin I switching in the developing heart.
    Schiaffino S
    The Journal of biological chemistry 264.27 (1989 Sep 25): 16299-302.

    Cardiac troponin T in developing, regenerating and denervated rat skeletal muscle.
    Schiaffino S
    Development (Cambridge, England) 110.2 (1990 Oct): 547-54.

    Troponin T- and troponin I-like proteins in bovine vascular smooth muscle.
    Sartore S
    Circulation research 68.5 (1991 May): 1349-61.

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    HSPB7 is indispensable for heart development by modulating actin filament assembly.
    Chen J
    Proceedings of the National Academy of Sciences of the United States of America 114.45 (2017 Nov 7): 11956-11961.

    IHC References

    Troponin T switching in the developing rat heart.
    Schiaffino S
    The Journal of biological chemistry 263.34 (1988 Dec 5): 18488-92.

    Transgenic systems for unequivocal identification of cardiac myocyte nuclei and analysis of cardiomyocyte cell cycle status.
    Hesse M
    Basic research in cardiology 110.3 (2015 May): 33.

    Casz1 is required for cardiomyocyte G1-to-S phase progression during mammalian cardiac development.
    Conlon FL
    Development (Cambridge, England) 142.11 (2015 Jun 1): 2037-47.

    FFPE References
    All References

    Troponin T switching in the developing rat heart.
    Schiaffino S
    The Journal of biological chemistry 263.34 (1988 Dec 5): 18488-92.

    Transgenic systems for unequivocal identification of cardiac myocyte nuclei and analysis of cardiomyocyte cell cycle status.
    Hesse M
    Basic research in cardiology 110.3 (2015 May): 33.

    Casz1 is required for cardiomyocyte G1-to-S phase progression during mammalian cardiac development.
    Conlon FL
    Development (Cambridge, England) 142.11 (2015 Jun 1): 2037-47.

    Transplantation of Adipose-Derived Stem Cell Sheet Attenuates Adverse Cardiac Remodeling in Acute Myocardial Infarction.
    Lim DS
    Tissue engineering. Part A 23.1-2 (2017 Jan): 1-11.

    Cardiac troponin T in developing, regenerating and denervated rat skeletal muscle.
    Schiaffino S
    Development (Cambridge, England) 110.2 (1990 Oct): 547-54.

    HSPB7 is indispensable for heart development by modulating actin filament assembly.
    Chen J
    Proceedings of the National Academy of Sciences of the United States of America 114.45 (2017 Nov 7): 11956-11961.

    Transplantation of Immortalized CD34+ and CD34- Adipose-Derived Stem Cells Improve Cardiac Function and Mitigate Systemic Pro-Inflammatory Responses.
    Lim DS
    PloS one 11.2 (2016): e0147853.

    Biomimetic Cardiac Tissue Model Enables the Adaption of Human Induced Pluripotent Stem Cell Cardiomyocytes to Physiological Hemodynamic Loads.
    Sethu P
    Analytical chemistry 88.19 (2016 Oct 4): 9862-9868.

    Troponin T- and troponin I-like proteins in bovine vascular smooth muscle.
    Sartore S
    Circulation research 68.5 (1991 May): 1349-61.

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement.
    Lim DS
    ACS applied materials & interfaces 9.20 (2017 May 24): 16803-16812.

    Isolation of contractile cardiomyocytes from human pluripotent stem-cell-derived cardiomyogenic cultures using a human NCX1-EGFP reporter.
    Wolvetang EJ
    Stem cells and development 24.1 (2015 Jan 1): 11-20.

    Preventing permeability transition pore opening increases mitochondrial maturation, myocyte differentiation and cardiac function in the neonatal mouse heart.
    Porter GA Jr
    Pediatric research 81.6 (2017 Jun): 932-941.

    Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction.
    Lim DS
    PloS one 11.6 (2016): e0158067.

    Troponin I switching in the developing heart.
    Schiaffino S
    The Journal of biological chemistry 264.27 (1989 Sep 25): 16299-302.

    Cardiomyocyte-specific conditional knockout of the histone chaperone HIRA in mice results in hypertrophy, sarcolemmal damage and focal replacement fibrosis.
    Stewart MD
    Disease models & mechanisms 9.3 (2016 Mar): 335-45.

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