CH1
Catalog Fields
Antigen: tropomyosin, striated muscle
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Depositors Institution: University of Iowa
Host Species: mouse
Gene: TPM1
Positive Tested Species Reactivity: Axolotl, Broad species, Chicken, Fish, Human, Mouse, Rat, Xenopus
Clonality: Monoclonal
Antigen Molecular Weight: Apparent: 39 kDa (or smaller depending on the isoform)
Epitope Mapped: Yes
Immunogen: Purified chicken cardiac muscle tropomyosin
Epitope Location or Sequence: CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].
Myeloma Strain: NS1
Immunogen Sequence: Full length protein
Uniprot ID: P04268
Additional Information: CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.
Entrez Gene ID: 396366
Antibody Registry ID: AB_2205770
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
30 References
Initial Publication
Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
Lin JL
Hybridoma 4.3 (1985 Fall): 223-42.