CH1
Catalog Fields
Antigen: Tropomyosin, alpha-1 chain (striated muscle)
Available to For-Profits: Yes
Antigen Species: Chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Lin, J.J.-C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Axolotl, Broad species, Chicken, Fish, Human, Mouse, Rat, Xenopus, Zebrafish
Depositors Institution: University of Iowa
Antigen Molecular Weight: Apparent: 39 kDa (or smaller depending on the isoform)
Depositors Notes:
Immunogen: Purified chicken cardiac muscle tropomyosin
Gene: TPM1
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: P04268
Epitope Location or Sequence: CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].
Entrez Gene ID: 396366
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2205770
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
31 References
Initial Publication
Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
Lin JL
Hybridoma 4.3 (1985 Fall): 223-42.