CH1

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$40.00
SKU: CH1
View product citations for antibody CH1 on CiteAb

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Available: 174

DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin, striated muscle
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Axolotl, Broad species, Chicken, Fish, Human, Mouse, Rat, Xenopus
Depositors Notes:
Antigen Molecular Weight: Apparent: 39 kDa (or smaller depending on the isoform)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: TPM1
Immunogen: Purified chicken cardiac muscle tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].
Uniprot ID: P04268 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396366 
Additional Characterization:
Antibody Registry ID: AB_2205770 
Additional Information: CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

30 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Expression of a novel cardiac-specific tropomyosin isoform in humans.
    Dube DK
    Biochemical and biophysical research communications 320.4 (2004 Aug 6): 1291-7.

    Ectopic expression of tropomyosin promotes myofibrillogenesis in mutant axolotl hearts.
    Dube DK
    Developmental dynamics : an official publication of the American Association of Anatomists 213.4 (1998 Dec): 412-20.

    Talin1 is required for cardiac Z-disk stabilization and endothelial integrity in zebrafish.
    Xiong JW
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29.12 (2015 Dec): 4989-5005.

    Egfl6 is involved in zebrafish notochord development.
    Liu D
    Fish physiology and biochemistry 41.4 (2015 Aug): 961-9.

    Cardiac troponin T is necessary for normal development in the embryonic chick heart.
    Loughna S
    Journal of anatomy 229.3 (2016 Sep): 436-49.

    Comparative morphology and development of extra-ocular muscles in the lamprey and gnathostomes reveal the ancestral state and developmental patterns of the vertebrate head.
    Wada H
    Zoological letters 2. (2016): 10.

    Expression of tropomyosin in relation to myofibrillogenesis in axolotl hearts.
    Dube DK
    Regenerative medicine research 1.1 (2013 Dec): 8.

    Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development.
    Conlon FL
    Developmental dynamics : an official publication of the American Association of Anatomists 243.7 (2014 Jul): 948-56.

    Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers.
    Zak R
    Histochemistry 93.4 (1990): 423-8.

    Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Confocal imaging of early heart development in Xenopus laevis.
    Weeks DL
    Developmental biology 218.1 (2000 Feb 1): 64-73.

    Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility.
    Song HY
    FEBS letters 579.21 (2005 Aug 29): 4843-50.

    Wortmannin induces zebrafish cardia bifida through a mechanism independent of phosphoinositide 3-kinase and myosin light chain kinase.
    Song H
    Biochemical and biophysical research communications 331.1 (2005 May 27): 303-8.

    WB References

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines.
    Poiesz BJ
    International journal of breast cancer 2015. (2015): 859427.

    Expression of sarcomeric tropomyosin in striated muscles in axolotl treated with shz-1, a small cardiogenic molecule.
    Dube DK
    Cardiovascular toxicology 15.1 (2015 Jan): 29-40.

    Postmortem degradation of skeletal muscle proteins: a novel approach to determine the time since death.
    Steinbacher P
    International journal of legal medicine 130.2 (2016 Mar): 421-31.

    The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy.
    Yamaji R
    Biochemical and biophysical research communications 478.3 (2016 Sep 23): 1292-7.

    Slow recovery of the impaired fatigue resistance in postunloading mouse soleus muscle corresponding to decreased mitochondrial function and a compensatory increase in type I slow fibers.
    Jin JP
    American journal of physiology. Cell physiology 310.1 (2016 Jan 1): C27-40.

    Expression of tropomyosin in relation to myofibrillogenesis in axolotl hearts.
    Dube DK
    Regenerative medicine research 1.1 (2013 Dec): 8.

    Molecular and physiological effects of overexpressing striated muscle beta-tropomyosin in the adult murine heart.
    Wieczorek DF
    The Journal of biological chemistry 270.51 (1995 Dec 22): 30593-603.

    Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro.
    Lin JJ
    The Journal of cell biology 109.2 (1989 Aug): 495-504.

    Tropomyosin isoform expression and phosphorylation in the human heart in health and disease.
    Ward DG
    Journal of muscle research and cell motility 34.3-4 (2013 Aug): 189-97.

    Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function.
    Lang CH
    Alcoholism, clinical and experimental research 43.10 (2019 Oct): 2090-2099.

    IP References

    Assembly of tropomyosin isoforms into the cytoskeleton of avian muscle cells.
    Fulton AB
    Pediatric research 43.6 (1998 Jun): 813-22.

    ELISA References
    FB References
    Epitope Map References

    Expression of sarcomeric tropomyosin in striated muscles in axolotl treated with shz-1, a small cardiogenic molecule.
    Dube DK
    Cardiovascular toxicology 15.1 (2015 Jan): 29-40.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    All References

    Expression of a novel cardiac-specific tropomyosin isoform in humans.
    Dube DK
    Biochemical and biophysical research communications 320.4 (2004 Aug 6): 1291-7.

    Ectopic expression of tropomyosin promotes myofibrillogenesis in mutant axolotl hearts.
    Dube DK
    Developmental dynamics : an official publication of the American Association of Anatomists 213.4 (1998 Dec): 412-20.

    Talin1 is required for cardiac Z-disk stabilization and endothelial integrity in zebrafish.
    Xiong JW
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29.12 (2015 Dec): 4989-5005.

    Egfl6 is involved in zebrafish notochord development.
    Liu D
    Fish physiology and biochemistry 41.4 (2015 Aug): 961-9.

    Cardiac troponin T is necessary for normal development in the embryonic chick heart.
    Loughna S
    Journal of anatomy 229.3 (2016 Sep): 436-49.

    Comparative morphology and development of extra-ocular muscles in the lamprey and gnathostomes reveal the ancestral state and developmental patterns of the vertebrate head.
    Wada H
    Zoological letters 2. (2016): 10.

    Expression of tropomyosin in relation to myofibrillogenesis in axolotl hearts.
    Dube DK
    Regenerative medicine research 1.1 (2013 Dec): 8.

    Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development.
    Conlon FL
    Developmental dynamics : an official publication of the American Association of Anatomists 243.7 (2014 Jul): 948-56.

    Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers.
    Zak R
    Histochemistry 93.4 (1990): 423-8.

    Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Confocal imaging of early heart development in Xenopus laevis.
    Weeks DL
    Developmental biology 218.1 (2000 Feb 1): 64-73.

    Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility.
    Song HY
    FEBS letters 579.21 (2005 Aug 29): 4843-50.

    Wortmannin induces zebrafish cardia bifida through a mechanism independent of phosphoinositide 3-kinase and myosin light chain kinase.
    Song H
    Biochemical and biophysical research communications 331.1 (2005 May 27): 303-8.

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines.
    Poiesz BJ
    International journal of breast cancer 2015. (2015): 859427.

    Expression of sarcomeric tropomyosin in striated muscles in axolotl treated with shz-1, a small cardiogenic molecule.
    Dube DK
    Cardiovascular toxicology 15.1 (2015 Jan): 29-40.

    Postmortem degradation of skeletal muscle proteins: a novel approach to determine the time since death.
    Steinbacher P
    International journal of legal medicine 130.2 (2016 Mar): 421-31.

    The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy.
    Yamaji R
    Biochemical and biophysical research communications 478.3 (2016 Sep 23): 1292-7.

    Slow recovery of the impaired fatigue resistance in postunloading mouse soleus muscle corresponding to decreased mitochondrial function and a compensatory increase in type I slow fibers.
    Jin JP
    American journal of physiology. Cell physiology 310.1 (2016 Jan 1): C27-40.

    Molecular and physiological effects of overexpressing striated muscle beta-tropomyosin in the adult murine heart.
    Wieczorek DF
    The Journal of biological chemistry 270.51 (1995 Dec 22): 30593-603.

    Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro.
    Lin JJ
    The Journal of cell biology 109.2 (1989 Aug): 495-504.

    Tropomyosin isoform expression and phosphorylation in the human heart in health and disease.
    Ward DG
    Journal of muscle research and cell motility 34.3-4 (2013 Aug): 189-97.

    Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function.
    Lang CH
    Alcoholism, clinical and experimental research 43.10 (2019 Oct): 2090-2099.

    Assembly of tropomyosin isoforms into the cytoskeleton of avian muscle cells.
    Fulton AB
    Pediatric research 43.6 (1998 Jun): 813-22.

    N-Terminal Hypervariable Region of Muscle Type Isoforms of Troponin T Differentially Modulates the Affinity of Tropomyosin-Binding Site 1.
    Jin JP
    Biochemistry 54.24 (2015 Jun 23): 3822-30.

    Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage.
    Jin JP
    Biochemistry 45.38 (2006 Sep 26): 11681-94.

    Functional Basis of Three New Recessive Mutations of Slow Skeletal Muscle Troponin T Found in Non-Amish TNNT1 Nemaline Myopathies.
    Jin JP
    Biochemistry 55.32 (2016 Aug 16): 4560-7.

    Troponin T core structure and the regulatory NH2-terminal variable region.
    Jin JP
    Biochemistry 46.5 (2007 Feb 6): 1368-79.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

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