TI-1

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$40.00
SKU: TI-1
View product citations for antibody TI-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: troponin I, cardiac
Hybridoma Cells Available: Yes
Antigen Species: rabbit
Depositor: Schiaffino, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Universita degli Studi di Padova
Positive Tested Species Reactivity: Chick, Mouse, Rat, Squirrel
Depositors Notes: Immunoblotting note: cardiac troponin I. IHC note: cardiac fibers.
Antigen Molecular Weight: 24.1 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TNNI3
Immunogen: myofibrillar preparation; hypothyroid rabbit ventricle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: P02646 
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TI-1 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product TI-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Troponin I switching in the developing heart.
    Schiaffino S
    The Journal of biological chemistry 264.27 (1989 Sep 25): 16299-302.

    IF References
    WB References
    IHC References
    All References

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