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SKU: VIN-IS-53-s
View product citations for antibody VIN-IS-53 on CiteAb

In Stock

Available: 64

DSHB Data Sheet

Catalog Fields

Antigen: Neural Cell Adhesion Molecular/NCAM
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Andrews, P.W.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sheffield
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This antibody has been shown to be effective for immunopanning to isolate hiPSC immature neurons (PMID: 25837485)
Antigen Molecular Weight: 94.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: NCAM1
Immunogen: NTERA-2 cells induced to differentiate by retinoic acid
Alternate Gene Names: CD56, NCAM
Alternate Antibody Name: CD56
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P13591 
Immunogen Sequence: Cell lysate
Entrez Gene ID: 4684 
Additional Characterization:  
Antibody Registry ID: AB_2149688 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VIN-IS-53 was deposited to the DSHB by Andrews, P.W. (DSHB Hybridoma Product VIN-IS-53)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    The immunohistochemical profile of the myelomeningocele placode: is the placode normal?
    Cummings TJ
    Pediatric neurosurgery 39.5 (2003 Nov): 234-9.

    The immunohistochemical profile of the tethered filum terminale.
    Cummings TJ
    Pediatric neurosurgery 39.5 (2003 Nov): 227-33.

    All References

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