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SKU: 5A5
View product citations for antibody 5A5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: NCAM (sialylated form); PSA-NCAM
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgM
Depositors Institution: HHMI/Columbia University
Host Species: mouse
Depositors Notes: The 5A5 antibody has successfully been used as a panning substrate for human neural stem cells attachment (PMID: 27680492).
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Gene: Ncam1
Antigen Molecular Weight: Apparent: 300kD
Alternate Gene Names: Cd56/N-CAM/N-CAM-1/NCAM-1/NCAM-C/NCAMC/Ncam
Immunogen: Embryonic spinal cord membranes (rat)
Clonality: Monoclonal
Alternate Antibody Name: CD56
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: polysialic acid
Uniprot ID: P13596 
Additional Information: This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.
Entrez Gene ID: 24586 
Antibody Registry ID: AB_2314873 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5A5 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References

    NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
    Rutishauser U
    The Journal of cell biology 114.1 (1991 Jul): 143-53.

    All References

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