Antigen: NCAM (sialylated form); PSA-NCAM
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Depositors Notes: The 5A5 antibody has successfully been used as a panning substrate for human neural stem cells attachment (PMID: 27680492).
Antigen Molecular Weight: Apparent: 300kD
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Embryonic spinal cord membranes (rat)
Alternate Gene Names: Cd56/N-CAM/N-CAM-1/NCAM-1/NCAM-C/NCAMC/Ncam
Alternate Antibody Name: CD56
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: polysialic acid
Uniprot ID: P13596
Entrez Gene ID: 24586
Antibody Registry ID: AB_2314873
Additional Information: This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5A5 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References