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SKU: 5A5

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 5A5
Available to For-Profits: Yes
Alternate Antibody Name: CD56
Gene Name: Ncam1
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_528392 
Uniprot ID: P13596 
Entrez Gene ID: 24586 
Clonality: Monoclonal
Immunogen: Embryonic spinal cord membranes (rat)
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: NCAM (sialylated form); PSA-NCAM
Epitope Location or Sequence: polysialic acid
Alternate Antigen Name:
Deposit Date: 3/15/1989
Antigen Molecular Weight: Apparent: 300kD
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University / HHMI
Antigen Species: Rat
Depositor Notes: The 5A5 antibody has successfully been used as a panning substrate for human neural stem cells attachment (PMID: 27680492).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Mouse, Rat
Additional Information: This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5A5 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References

    NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
    Rutishauser U
    The Journal of cell biology 114.1 (1991 Jul): 143-53.

    All References

    Loss of neural recognition molecule NB-3 delays the normal projection and terminal branching of developing corticospinal tract axons in the mouse.
    Liu Y
    The Journal of comparative neurology 520.6 (2012 Apr 15): 1227-45.

    Neurochemical Characterization of PSA-NCAM(+) Cells in the Human Brain and Phenotypic Quantification in Alzheimer's Disease Entorhinal Cortex.
    Curtis MA
    Neuroscience 372. (2018 Feb 21): 289-303.

    Comparison of pure and mixed populations of human fetal-derived neural progenitors transplanted into intact adult rat brain.
    Tresco PA
    Experimental neurology 184.2 (2003 Dec): 816-29.

    Developmental expression of N-CAM epitopes in the rat spinal cord during corticospinal tract axon outgrowth and target innervation.
    Joosten EA
    Brain research. Developmental brain research 78.2 (1994 Apr 15): 226-36.

    Differential Fatty Acid-Binding Protein Expression in Persistent Radial Glia in the Human and Sheep Subventricular Zone.
    Curtis MA
    Developmental neuroscience 40.2 (2018): 145-161.

    Hippocampal neurons isolated from rats subjected to the valproic acid model mimic in vivo synaptic pattern: evidence of neuronal priming during early development in autism spectrum disorders.
    Reinés A
    Molecular autism 12.1 (2021 Mar 6): 23.

    Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons.
    Khalili K
    Methods in molecular biology (Clifton, N.J.) 1078. (2013): 45-54.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

    NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
    Rutishauser U
    The Journal of cell biology 114.1 (1991 Jul): 143-53.

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