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SKU: 5A5
View product citations for antibody 5A5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: NCAM (sialylated form); PSA-NCAM
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Depositors Notes: The 5A5 antibody has successfully been used as a panning substrate for human neural stem cells attachment (PMID: 27680492).
Antigen Molecular Weight: Apparent: 300kD
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Ncam1
Immunogen: Embryonic spinal cord membranes (rat)
Alternate Gene Names: Cd56/N-CAM/N-CAM-1/NCAM-1/NCAM-C/NCAMC/Ncam
Alternate Antibody Name: CD56
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: polysialic acid
Uniprot ID: P13596 
Immunogen Sequence:
Entrez Gene ID: 24586 
Additional Characterization:  
Antibody Registry ID: AB_2314873 
Additional Information: This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5A5 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 5A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References

    NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
    Rutishauser U
    The Journal of cell biology 114.1 (1991 Jul): 143-53.

    All References

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