rr1

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$40.00
SKU: rr1
View product citations for antibody rr1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin, E- (canine)
Hybridoma Cells Available: Yes
Antigen Species: canine
Depositor: Gumbiner, B.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cell Biology, School of Medicine, University of Virginia
Positive Tested Species Reactivity: Canine
Depositors Notes: rr1 is canine specific. Blocks epithelial cell-cell adhesion and cell junction formation. Stains all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Antigen Molecular Weight: Predicted: 80 kDa (mature E-cadherin); Apparent 118 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CDH1
Immunogen: Madin Darby Canine Kidney (MDCK) cell line, whole cells
Alternate Gene Names: uvo, CD324
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Ag8
Epitope Location or Sequence: Extracellular domain 81 kDa tryptic fragment
Uniprot ID: F1PAA9 
Immunogen Sequence: Full length protein
Entrez Gene ID: 442858 
Additional Characterization:  
Antibody Registry ID: AB_528114 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rr1 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product rr1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide.
    Simons K
    The Journal of cell biology 102.2 (1986 Feb): 457-68.

    Selective degradation of E-cadherin and dissolution of E-cadherin-catenin complexes in epithelial ischemia.
    Nigam SK
    American journal of physiology. Renal physiology 278.5 (2000 May): F847-52.

    E-cadherin-mediated cell coupling is required for apoptotic cell extrusion.
    Bar-Sagi D
    Current biology : CB 24.8 (2014 Apr 14): 868-74.

    Extracellular cleavage of E-cadherin promotes epithelial cell extrusion.
    Rabouille C
    Journal of cell science 127.Pt 15 (2014 Aug 1): 3331-46.

    IP References
    FB References
    Epitope Map References
    All References

    A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide.
    Simons K
    The Journal of cell biology 102.2 (1986 Feb): 457-68.

    E-cadherin-mediated cell coupling is required for apoptotic cell extrusion.
    Bar-Sagi D
    Current biology : CB 24.8 (2014 Apr 14): 868-74.

    Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex.
    Nelson WJ
    Nature communications 8. (2017 Jan 3): 13996.

    Extracellular cleavage of E-cadherin promotes epithelial cell extrusion.
    Rabouille C
    Journal of cell science 127.Pt 15 (2014 Aug 1): 3331-46.

    p120-catenin controls contractility along the vertical axis of epithelial lateral membranes.
    Reynolds AB
    Journal of cell science 129.1 (2016 Jan 1): 80-94.

    Casein kinase-mediated phosphorylation of serine 839 is necessary for basolateral localization of the Ca²⁺-activated non-selective cation channel TRPM4.
    Stutzin A
    Pflugers Archiv : European journal of physiology 467.8 (2015 Aug): 1723-1732.

    MDCK cells are capable of water secretion and reabsorption in response to changes in the ionic environment.
    Eskelinen SM
    Canadian journal of physiology and pharmacology 95.1 (2017 Jan): 72-83.

    A phosphoinositide 3-kinase (PI3K)-serum- and glucocorticoid-inducible kinase 1 (SGK1) pathway promotes Kv7.1 channel surface expression by inhibiting Nedd4-2 protein.
    Rasmussen HB
    The Journal of biological chemistry 288.52 (2013 Dec 27): 36841-54.

    Selective degradation of E-cadherin and dissolution of E-cadherin-catenin complexes in epithelial ischemia.
    Nigam SK
    American journal of physiology. Renal physiology 278.5 (2000 May): F847-52.

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