rr1

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$40.00
SKU: rr1
View product citations for antibody rr1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin, E- (canine)
Hybridoma Cells Available: Yes
Antigen Species: canine
Depositor: Gumbiner, B.M.
Isotype: MIgG1
Depositors Institution: Cell Biology, School of Medicine, University of Virginia
Host Species: mouse
Depositors Notes: rr1 is canine specific. Blocks epithelial cell-cell adhesion and cell junction formation. Stains all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Positive Tested Species Reactivity: Canine
Gene: CDH1
Antigen Molecular Weight: Predicted: 80 kDa (mature E-cadherin); Apparent 118 kDa
Alternate Gene Names: uvo, CD324
Immunogen: Madin Darby Canine Kidney (MDCK) cell line, whole cells
Clonality: Monoclonal
Myeloma Strain: Ag8
Epitope Mapped: No
Uniprot ID: F1PAA9 
Epitope Location or Sequence: Extracellular domain 81 kDa tryptic fragment
Entrez Gene ID: 442858 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528114 
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rr1 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product rr1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide.
    Simons K
    The Journal of cell biology 102.2 (1986 Feb): 457-68.

    Selective degradation of E-cadherin and dissolution of E-cadherin-catenin complexes in epithelial ischemia.
    Nigam SK
    American journal of physiology. Renal physiology 278.5 (2000 May): F847-52.

    E-cadherin-mediated cell coupling is required for apoptotic cell extrusion.
    Bar-Sagi D
    Current biology : CB 24.8 (2014 Apr 14): 868-74.

    Extracellular cleavage of E-cadherin promotes epithelial cell extrusion.
    Rabouille C
    Journal of cell science 127.Pt 15 (2014 Aug 1): 3331-46.

    IP References
    FB References
    Epitope Map References
    All References

    A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide.
    Simons K
    The Journal of cell biology 102.2 (1986 Feb): 457-68.

    E-cadherin-mediated cell coupling is required for apoptotic cell extrusion.
    Bar-Sagi D
    Current biology : CB 24.8 (2014 Apr 14): 868-74.

    Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex.
    Nelson WJ
    Nature communications 8. (2017 Jan 3): 13996.

    Extracellular cleavage of E-cadherin promotes epithelial cell extrusion.
    Rabouille C
    Journal of cell science 127.Pt 15 (2014 Aug 1): 3331-46.

    p120-catenin controls contractility along the vertical axis of epithelial lateral membranes.
    Reynolds AB
    Journal of cell science 129.1 (2016 Jan 1): 80-94.

    Casein kinase-mediated phosphorylation of serine 839 is necessary for basolateral localization of the Ca²⁺-activated non-selective cation channel TRPM4.
    Stutzin A
    Pflugers Archiv : European journal of physiology 467.8 (2015 Aug): 1723-1732.

    MDCK cells are capable of water secretion and reabsorption in response to changes in the ionic environment.
    Eskelinen SM
    Canadian journal of physiology and pharmacology 95.1 (2017 Jan): 72-83.

    A phosphoinositide 3-kinase (PI3K)-serum- and glucocorticoid-inducible kinase 1 (SGK1) pathway promotes Kv7.1 channel surface expression by inhibiting Nedd4-2 protein.
    Rasmussen HB
    The Journal of biological chemistry 288.52 (2013 Dec 27): 36841-54.

    Selective degradation of E-cadherin and dissolution of E-cadherin-catenin complexes in epithelial ischemia.
    Nigam SK
    American journal of physiology. Renal physiology 278.5 (2000 May): F847-52.

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