Catalog Fields
Antigen: Vimentin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fulton, A.B.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Human, Porcine, Rabbit
Depositors Notes: AMF-17b recognizes vimentin in all mammal species tested except rodents.
Antigen Molecular Weight: Predicted: 53kDa; Apparent: 53kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: VIM
Immunogen: Embryonic myofibril
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: Located close (upstream) to amino acid position 220
Uniprot ID: P09654
Immunogen Sequence: Full length protein
Entrez Gene ID: 420519
Additional Characterization:
Antibody Registry ID: AB_528505
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AMF-17b was deposited to the DSHB by Fulton, A.B. (DSHB Hybridoma Product AMF-17b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
31 References
Initial Publication
Assembly of vimentin in cultured cells varies with cell type.
Fulton AB
The Journal of biological chemistry 264.30 (1989 Oct 25): 17953-60.