AMF-17b
Catalog Fields
Antigen: Vimentin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fulton, A.B.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Human, Porcine, Rabbit
Depositors Notes: AMF-17b recognizes vimentin in all mammal species tested except rodents.
Predicted Species Reactivity:
Gene: VIM
Immunogen: Embryonic myofibril
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: Located close (upstream) to amino acid position 220
Uniprot ID: P09654
Immunogen Sequence: Full length protein
Entrez Gene ID: 420519
Antibody Registry ID: AB_528505
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AMF-17b was deposited to the DSHB by Fulton, A.B. (DSHB Hybridoma Product AMF-17b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
31 References
Initial Publication
Assembly of vimentin in cultured cells varies with cell type.
Fulton AB
The Journal of biological chemistry 264.30 (1989 Oct 25): 17953-60.