AMF-17b

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$40.00
SKU: AMF-17b
View product citations for antibody AMF-17b on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Vimentin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fulton, A.B.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Bovine, Canine, Chicken, Human, Porcine, Rabbit
Depositors Notes: AMF-17b recognizes vimentin in all mammal species tested except rodents.
Antigen Molecular Weight: Predicted: 53kDa; Apparent: 53kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: VIM
Immunogen: Embryonic myofibril
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: Located close (upstream) to amino acid position 220
Uniprot ID: P09654 
Immunogen Sequence: Full length protein
Entrez Gene ID: 420519 
Additional Characterization:
Antibody Registry ID: AB_528505 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AMF-17b was deposited to the DSHB by Fulton, A.B. (DSHB Hybridoma Product AMF-17b)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

31 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Assembly of vimentin in cultured cells varies with cell type.
    Fulton AB
    The Journal of biological chemistry 264.30 (1989 Oct 25): 17953-60.

    IF References

    Molecular characteristics of the novel intermediate filament protein paranemin. Sequence reveals EAP-300 and IFAPa-400 are highly homologous to paranemin.
    Robson RM
    The Journal of biological chemistry 272.51 (1997 Dec 19): 32489-99.

    Association of titin and myosin heavy chain in developing skeletal muscle.
    Fulton AB
    Proceedings of the National Academy of Sciences of the United States of America 89.16 (1992 Aug 15): 7496-500.

    Contribution of the primitive epicardium to the subepicardial mesenchyme in hamster and chick embryos.
    Muñoz-Chápuli R
    Developmental dynamics : an official publication of the American Association of Anatomists 210.2 (1997 Oct): 96-105.

    Modulation of pluripotency in the porcine embryo and iPS cells.
    Alberio R
    PloS one 7.11 (2012): e49079.

    Osteocyte shape is dependent on actin filaments and osteocyte processes are unique actin-rich projections.
    Lim SS
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 13.10 (1998 Oct): 1555-68.

    Molecular characteristics and interactions of the intermediate filament protein synemin. Interactions with alpha-actinin may anchor synemin-containing heterofilaments.
    Robson RM
    The Journal of biological chemistry 274.41 (1999 Oct 8): 29493-9.

    The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes.
    Guilak F
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 22.1 (2004 Jan): 131-9.

    Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta.
    Muñoz-Chápuli R
    The Histochemical journal 31.12 (1999 Dec): 771-9.

    Isolation of murine and porcine fetal stem cells from somatic tissue.
    Niemann H
    Biology of reproduction 72.4 (2005 Apr): 1020-8.

    M cells in the follicle-associated epithelium of the rabbit conjunctiva preferentially bind and translocate latex beads.
    Phillips TE
    Investigative ophthalmology and visual science 46.11 (2005 Nov): 4217-23.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens.
    George-Weinstein M
    PloS one 9.4 (2014): e95262.

    Functional effects of adult human olfactory stem cells on early-onset sensorineural hearing loss.
    Oleskevich S
    Stem cells (Dayton, Ohio) 29.4 (2011 Apr): 670-7.

    Epicardial-like cells on the distal arterial end of the cardiac outflow tract do not derive from the proepicardium but are derivatives of the cephalic pericardium.
    Wessels A
    Developmental dynamics : an official publication of the American Association of Anatomists 227.1 (2003 May): 56-68.

    WB References
    IHC References

    Atrophy of Müller glia and photoreceptor cells in chick retina misexpressing cNSCL2.
    Wang SZ
    Investigative ophthalmology and visual science 42.13 (2001 Dec): 3103-9.

    Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta.
    Muñoz-Chápuli R
    The Histochemical journal 31.12 (1999 Dec): 771-9.

    Differences in cartilage-forming capacity of expanded human chondrocytes from ear and nose and their gene expression profiles.
    van Osch GJ
    Cell transplantation 20.6 (2011): 925-40.

    Locally applied testosterone is a novel method to influence the development of the avian bursa of Fabricius.
    Oláh I
    Journal of immunological methods 343.2 (2009 Apr 15): 97-102.

    Pyloric tonsil as a novel gut-associated lymphoepithelial organ of the chicken.
    Oláh I
    Journal of anatomy 211.3 (2007 Sep): 407-11.

    In ovo vitelline duct ligation results in transient changes of bursal microenvironments.
    Magyar A
    Immunology 116.2 (2005 Oct): 267-75.

    An immunohistochemical study of the rabbit suprapatella, a sesamoid fibrocartilage in the quadriceps tendon containing aggrecan.
    Benjamin M
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.7 (2002 Jul): 955-60.

    Differentiation of hemangioblasts from embryonic mesothelial cells? A model on the origin of the vertebrate cardiovascular system.
    González M
    Differentiation; research in biological diversity 64.3 (1999 Mar): 133-41.

    Temporal and spatial expression of CCN3 during retina development.
    Marx M
    Developmental neurobiology 72.11 (2012 Nov): 1363-75.

    Oesophageal tonsil of the chicken.
    Oláh I
    Acta veterinaria Hungarica 53.2 (2005): 173-88.

    Muscularizing tissues in the endocardial cushions of the avian heart are characterized by the expression of h1-calponin.
    Wessels A
    Developmental dynamics : an official publication of the American Association of Anatomists 235.6 (2006 Jun): 1648-58.

    The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage.
    van Osch GJ
    European cells and materials 27. (2014 Apr 6): 264-80; discussion 278-80.

    IP References

    Assembly of vimentin in cultured cells varies with cell type.
    Fulton AB
    The Journal of biological chemistry 264.30 (1989 Oct 25): 17953-60.

    Association of titin and myosin heavy chain in developing skeletal muscle.
    Fulton AB
    Proceedings of the National Academy of Sciences of the United States of America 89.16 (1992 Aug 15): 7496-500.

    FFPE References

    Contribution of the primitive epicardium to the subepicardial mesenchyme in hamster and chick embryos.
    Muñoz-Chápuli R
    Developmental dynamics : an official publication of the American Association of Anatomists 210.2 (1997 Oct): 96-105.

    Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta.
    Muñoz-Chápuli R
    The Histochemical journal 31.12 (1999 Dec): 771-9.

    M cells in the follicle-associated epithelium of the rabbit conjunctiva preferentially bind and translocate latex beads.
    Phillips TE
    Investigative ophthalmology and visual science 46.11 (2005 Nov): 4217-23.

    Differentiation of hemangioblasts from embryonic mesothelial cells? A model on the origin of the vertebrate cardiovascular system.
    González M
    Differentiation; research in biological diversity 64.3 (1999 Mar): 133-41.

    Epicardial-like cells on the distal arterial end of the cardiac outflow tract do not derive from the proepicardium but are derivatives of the cephalic pericardium.
    Wessels A
    Developmental dynamics : an official publication of the American Association of Anatomists 227.1 (2003 May): 56-68.

    Oesophageal tonsil of the chicken.
    Oláh I
    Acta veterinaria Hungarica 53.2 (2005): 173-88.

    Muscularizing tissues in the endocardial cushions of the avian heart are characterized by the expression of h1-calponin.
    Wessels A
    Developmental dynamics : an official publication of the American Association of Anatomists 235.6 (2006 Jun): 1648-58.

    The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage.
    van Osch GJ
    European cells and materials 27. (2014 Apr 6): 264-80; discussion 278-80.

    Epitope Map References

    Assembly of vimentin in cultured cells varies with cell type.
    Fulton AB
    The Journal of biological chemistry 264.30 (1989 Oct 25): 17953-60.

    All References

    Atrophy of Müller glia and photoreceptor cells in chick retina misexpressing cNSCL2.
    Wang SZ
    Investigative ophthalmology and visual science 42.13 (2001 Dec): 3103-9.

    Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta.
    Muñoz-Chápuli R
    The Histochemical journal 31.12 (1999 Dec): 771-9.

    Differences in cartilage-forming capacity of expanded human chondrocytes from ear and nose and their gene expression profiles.
    van Osch GJ
    Cell transplantation 20.6 (2011): 925-40.

    Locally applied testosterone is a novel method to influence the development of the avian bursa of Fabricius.
    Oláh I
    Journal of immunological methods 343.2 (2009 Apr 15): 97-102.

    Pyloric tonsil as a novel gut-associated lymphoepithelial organ of the chicken.
    Oláh I
    Journal of anatomy 211.3 (2007 Sep): 407-11.

    In ovo vitelline duct ligation results in transient changes of bursal microenvironments.
    Magyar A
    Immunology 116.2 (2005 Oct): 267-75.

    An immunohistochemical study of the rabbit suprapatella, a sesamoid fibrocartilage in the quadriceps tendon containing aggrecan.
    Benjamin M
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.7 (2002 Jul): 955-60.

    Differentiation of hemangioblasts from embryonic mesothelial cells? A model on the origin of the vertebrate cardiovascular system.
    González M
    Differentiation; research in biological diversity 64.3 (1999 Mar): 133-41.

    Temporal and spatial expression of CCN3 during retina development.
    Marx M
    Developmental neurobiology 72.11 (2012 Nov): 1363-75.

    Oesophageal tonsil of the chicken.
    Oláh I
    Acta veterinaria Hungarica 53.2 (2005): 173-88.

    Muscularizing tissues in the endocardial cushions of the avian heart are characterized by the expression of h1-calponin.
    Wessels A
    Developmental dynamics : an official publication of the American Association of Anatomists 235.6 (2006 Jun): 1648-58.

    The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage.
    van Osch GJ
    European cells and materials 27. (2014 Apr 6): 264-80; discussion 278-80.

    Molecular characteristics of the novel intermediate filament protein paranemin. Sequence reveals EAP-300 and IFAPa-400 are highly homologous to paranemin.
    Robson RM
    The Journal of biological chemistry 272.51 (1997 Dec 19): 32489-99.

    Association of titin and myosin heavy chain in developing skeletal muscle.
    Fulton AB
    Proceedings of the National Academy of Sciences of the United States of America 89.16 (1992 Aug 15): 7496-500.

    Contribution of the primitive epicardium to the subepicardial mesenchyme in hamster and chick embryos.
    Muñoz-Chápuli R
    Developmental dynamics : an official publication of the American Association of Anatomists 210.2 (1997 Oct): 96-105.

    Modulation of pluripotency in the porcine embryo and iPS cells.
    Alberio R
    PloS one 7.11 (2012): e49079.

    Osteocyte shape is dependent on actin filaments and osteocyte processes are unique actin-rich projections.
    Lim SS
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 13.10 (1998 Oct): 1555-68.

    Molecular characteristics and interactions of the intermediate filament protein synemin. Interactions with alpha-actinin may anchor synemin-containing heterofilaments.
    Robson RM
    The Journal of biological chemistry 274.41 (1999 Oct 8): 29493-9.

    The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes.
    Guilak F
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 22.1 (2004 Jan): 131-9.

    Isolation of murine and porcine fetal stem cells from somatic tissue.
    Niemann H
    Biology of reproduction 72.4 (2005 Apr): 1020-8.

    M cells in the follicle-associated epithelium of the rabbit conjunctiva preferentially bind and translocate latex beads.
    Phillips TE
    Investigative ophthalmology and visual science 46.11 (2005 Nov): 4217-23.

    Peripheral blood fibrocytes contribute to the formation of the avian spleen.
    Oláh I
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 55-66.

    Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens.
    George-Weinstein M
    PloS one 9.4 (2014): e95262.

    Functional effects of adult human olfactory stem cells on early-onset sensorineural hearing loss.
    Oleskevich S
    Stem cells (Dayton, Ohio) 29.4 (2011 Apr): 670-7.

    Epicardial-like cells on the distal arterial end of the cardiac outflow tract do not derive from the proepicardium but are derivatives of the cephalic pericardium.
    Wessels A
    Developmental dynamics : an official publication of the American Association of Anatomists 227.1 (2003 May): 56-68.

    Assembly of vimentin in cultured cells varies with cell type.
    Fulton AB
    The Journal of biological chemistry 264.30 (1989 Oct 25): 17953-60.

    Targeting Fyn in Ras-transformed cells induces F-actin to promote adherens junction-mediated cell-cell adhesion.
    Denning MF
    Molecular carcinogenesis 54.10 (2015 Oct): 1181-93.

    Actin filament organization regulates the induction of lens cell differentiation and survival.
    Menko AS
    Developmental biology 295.2 (2006 Jul 15): 714-29.

    The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst.
    Mortara RA
    Parasitology 133.Pt 3 (2006 Sep): 321-9.

    Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development.
    Simon HG
    Developmental biology 302.1 (2007 Feb 1): 230-42.

    Progenitor cell isolation from muscle-derived cells based on adhesion properties.
    Cherel Y
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.6 (2007 Jun): 607-18.

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