4A6
Catalog Fields
Antigen: Myosin heavy chain, fast, extraocular specific (MYH13)
Hybridoma Cells Available: No
Antigen Species: cat
Depositor: Lucas, C.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Cat, Human, Rabbit, Rat
Depositors Notes: 4A6 reacts extraocular specific fast myosin heavy chain from rabbit extraocular muscles by western blot, and with extraocular specific fast muscle fibres on rabbit, rat and cat extraocular and laryngeal muscles by immunohistochemistry.
Predicted Species Reactivity:
Gene: MYH13
Immunogen: Extraocular muscle
Alternate Gene Names: MyHC-eom
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: M3WDH7
Immunogen Sequence: Full length protein
Entrez Gene ID: 101083886
Antibody Registry ID: AB_528282
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A6 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 4A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Expression of extraocular myosin heavy chain in rabbit laryngeal muscle.
Hoh JF
Journal of muscle research and cell motility 16.4 (1995 Aug): 368-78.