4A6

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$40.00
SKU: 4A6-s
View product citations for antibody 4A6 on CiteAb

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Available: 11

DSHB Data Sheet

Catalog Fields

Antigen: myosin heavy chain, fast, extraocular specific (MYH13)
Hybridoma Cells Available:
Antigen Species: cat
Depositor: Lucas, C.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Cat, Human, Rabbit, Rat
Depositors Notes: 4A6 reacts extraocular specific fast myosin heavy chain from rabbit extraocular muscles by western blot, and with extraocular specific fast muscle fibres on rabbit, rat and cat extraocular and laryngeal muscles by immunohistochemistry.
Antigen Molecular Weight: Predicted: 223 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH13
Immunogen: Extraocular muscle
Alternate Gene Names: MyHC-eom
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: M3WDH7 
Immunogen Sequence: Full length protein
Entrez Gene ID: 101083886 
Additional Characterization:
Antibody Registry ID: AB_528282 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A6 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 4A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Expression of extraocular myosin heavy chain in rabbit laryngeal muscle.
    Hoh JF
    Journal of muscle research and cell motility 16.4 (1995 Aug): 368-78.

    IF References

    The distribution of myosin heavy chain isoforms among rat extraocular muscle fiber types.
    Hoh JF
    Investigative ophthalmology and visual science 41.11 (2000 Oct): 3391-8.

    WB References

    Expression of extraocular myosin heavy chain in rabbit laryngeal muscle.
    Hoh JF
    Journal of muscle research and cell motility 16.4 (1995 Aug): 368-78.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Extraocular fast myosin heavy chain expression in the levator palpebrae and retractor bulbi muscles.
    Hoh JF
    Investigative ophthalmology and visual science 38.13 (1997 Dec): 2817-25.

    IHC References

    Expression of extraocular myosin heavy chain in rabbit laryngeal muscle.
    Hoh JF
    Journal of muscle research and cell motility 16.4 (1995 Aug): 368-78.

    Myosin heavy chain isoform expression in human extraocular muscles: longitudinal variation and patterns of expression in global and orbital layers.
    Oh SY
    Muscle and nerve 45.5 (2012 May): 713-20.

    Myosin heavy chain composition of the human genioglossus muscle.
    Sokoloff AJ
    Journal of speech, language, and hearing research : JSLHR 55.2 (2012 Apr): 609-25.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Extraocular fast myosin heavy chain expression in the levator palpebrae and retractor bulbi muscles.
    Hoh JF
    Investigative ophthalmology and visual science 38.13 (1997 Dec): 2817-25.

    Myosin heavy-chain composition of the human hyoglossus muscle.
    Li H
    Dysphagia 25.2 (2010 Jun): 81-93.

    Myosin heavy chain isoforms in human extraocular muscles.
    Pedrosa-Domellöf F
    Investigative ophthalmology and visual science 44.4 (2003 Apr): 1419-25.

    Distribution of developmental myosin heavy chains in adult rabbit extraocular muscle: identification of a novel embryonic isoform absent in fetal limb.
    Hoh JF
    Investigative ophthalmology and visual science 44.6 (2003 Jun): 2450-6.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles.
    Erzen I
    Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 247.11 (2009 Nov): 1505-15.

    All References

    Expression of extraocular myosin heavy chain in rabbit laryngeal muscle.
    Hoh JF
    Journal of muscle research and cell motility 16.4 (1995 Aug): 368-78.

    Myosin heavy chain isoform expression in human extraocular muscles: longitudinal variation and patterns of expression in global and orbital layers.
    Oh SY
    Muscle and nerve 45.5 (2012 May): 713-20.

    Myosin heavy chain composition of the human genioglossus muscle.
    Sokoloff AJ
    Journal of speech, language, and hearing research : JSLHR 55.2 (2012 Apr): 609-25.

    Expression of extraocular-superfast-myosin heavy chain in rat laryngeal muscles.
    Flint PW
    Neuroreport 9.16 (1998 Nov 16): 3639-42.

    Extraocular fast myosin heavy chain expression in the levator palpebrae and retractor bulbi muscles.
    Hoh JF
    Investigative ophthalmology and visual science 38.13 (1997 Dec): 2817-25.

    Myosin heavy-chain composition of the human hyoglossus muscle.
    Li H
    Dysphagia 25.2 (2010 Jun): 81-93.

    Myosin heavy chain isoforms in human extraocular muscles.
    Pedrosa-Domellöf F
    Investigative ophthalmology and visual science 44.4 (2003 Apr): 1419-25.

    Distribution of developmental myosin heavy chains in adult rabbit extraocular muscle: identification of a novel embryonic isoform absent in fetal limb.
    Hoh JF
    Investigative ophthalmology and visual science 44.6 (2003 Jun): 2450-6.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles.
    Erzen I
    Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 247.11 (2009 Nov): 1505-15.

    The distribution of myosin heavy chain isoforms among rat extraocular muscle fiber types.
    Hoh JF
    Investigative ophthalmology and visual science 41.11 (2000 Oct): 3391-8.

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