6H1

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$40.00
SKU: 6H1-s
View product citations for antibody 6H1 on CiteAb

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Available: 193

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain, fast, 2X
Hybridoma Cells Available:
Antigen Species: Rabbit
Depositor: Lucas, C.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Feline, Guinea Pig, Human, Mouse, Rabbit, Rat
Depositors Notes: This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.
Antigen Molecular Weight: ~200 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH1
Immunogen: Retractor bulbi (adult rabbit) crude muscle extract muscle co-absorbed with adjuvant peptide to gold particles as immunogen carrier.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q9TSE5 
Immunogen Sequence:
Entrez Gene ID: 100125991 
Additional Characterization:
Antibody Registry ID: AB_2314830 
Additional Information: 6H1 has been successfully used to stain skeletal muscle paraffin sections. Low pH antigen retrieval and pre-treatment with Proteinase K is required. See PUBMED ID: 24924099.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6H1 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 6H1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

20 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    IF References

    Fibre type-specific satellite cell response to aerobic training in sedentary adults.
    Peterson CA
    The Journal of physiology 592.12 (2014 Jun 15): 2625-35.

    Fibre-specific responses to endurance and low volume high intensity interval training: striking similarities in acute and chronic adaptation.
    Gurd BJ
    PloS one 9.6 (2014): e98119.

    Resveratrol supplementation does not augment performance adaptations or fibre-type-specific responses to high-intensity interval training in humans.
    Gurd BJ
    Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 39.11 (2014 Nov): 1305-13.

    Myostatin is a key mediator between energy metabolism and endurance capacity of skeletal muscle.
    Amthor H
    American journal of physiology. Regulatory, integrative and comparative physiology 307.4 (2014 Aug 15): R444-54.

    Muscle histopathology in nebulin-related nemaline myopathy: ultrastrastructural findings correlated to disease severity and genotype.
    Romero NB
    Acta neuropathologica communications 2. (2014 Apr 12): 44.

    Tetanus toxin preserves skeletal muscle contractile force and size during limb immobilization.
    Fishman PS
    Muscle and nerve 50.5 (2014 Nov): 759-66.

    The histone code reader Spin1 controls skeletal muscle development.
    Schüle R
    Cell death and disease 8.11 (2017 Nov 23): e3173.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging.
    Mann M
    Cell reports 19.11 (2017 Jun 13): 2396-2409.

    Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity.
    Stone MH
    Sports (Basel, Switzerland) 7.7 (2019 Jul 11): .

    WB References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity.
    Stone MH
    Sports (Basel, Switzerland) 7.7 (2019 Jul 11): .

    IHC References
    FFPE References
    All References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Contractile abnormalities of mouse muscles expressing hyperkalemic periodic paralysis mutant NaV1.4 channels do not correlate with Na+ influx or channel content.
    Renaud JM
    Physiological genomics 46.11 (2014 Jun 1): 385-97.

    The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans.
    Hepple RT
    PloS one 9.8 (2014): e103044.

    Different Training-Induced Skeletal Muscle Adaptations in COPD Patients with and without Alpha-1 Antitrypsin Deficiency.
    Kenn K
    Respiration; international review of thoracic diseases 92.5 (2016): 339-347.

    Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study.
    Peterson CA
    Journal of translational medicine 14.1 (2016 Sep 29): 284.

    Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice.
    Kurland IJ
    Veterinary pathology 56.2 (2019 Mar): 322-331.

    Fidelity of muscle fibre reinnervation modulates ageing muscle impact in elderly women.
    Hepple RT
    The Journal of physiology 597.19 (2019 Oct): 5009-5023.

    Fibre type-specific satellite cell response to aerobic training in sedentary adults.
    Peterson CA
    The Journal of physiology 592.12 (2014 Jun 15): 2625-35.

    Fibre-specific responses to endurance and low volume high intensity interval training: striking similarities in acute and chronic adaptation.
    Gurd BJ
    PloS one 9.6 (2014): e98119.

    Resveratrol supplementation does not augment performance adaptations or fibre-type-specific responses to high-intensity interval training in humans.
    Gurd BJ
    Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 39.11 (2014 Nov): 1305-13.

    Myostatin is a key mediator between energy metabolism and endurance capacity of skeletal muscle.
    Amthor H
    American journal of physiology. Regulatory, integrative and comparative physiology 307.4 (2014 Aug 15): R444-54.

    Muscle histopathology in nebulin-related nemaline myopathy: ultrastrastructural findings correlated to disease severity and genotype.
    Romero NB
    Acta neuropathologica communications 2. (2014 Apr 12): 44.

    Tetanus toxin preserves skeletal muscle contractile force and size during limb immobilization.
    Fishman PS
    Muscle and nerve 50.5 (2014 Nov): 759-66.

    The histone code reader Spin1 controls skeletal muscle development.
    Schüle R
    Cell death and disease 8.11 (2017 Nov 23): e3173.

    Differential impact of tongue exercise on intrinsic lingual muscles.
    Connor NP
    The Laryngoscope 128.10 (2018 Oct): 2245-2251.

    BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration.
    Xie L
    Biochemical and biophysical research communications 509.1 (2019 Jan 29): 125-132.

    Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging.
    Mann M
    Cell reports 19.11 (2017 Jun 13): 2396-2409.

    Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity.
    Stone MH
    Sports (Basel, Switzerland) 7.7 (2019 Jul 11): .

    Reliable determination of training-induced alterations in muscle fiber composition in human skeletal muscle using quantitative polymerase chain reaction.
    Rønnestad BR
    Scandinavian journal of medicine and science in sports 24.5 (2014 Oct): e332-42.

    Immunohistochemical analysis of laryngeal muscles in normal horses and horses with subclinical recurrent laryngeal neuropathy.
    Hoh JF
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.8 (2009 Aug): 787-800.

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