2F7
Catalog Fields
Product Name/ID: 2F7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYH2
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_1157865
Uniprot ID: M3WDH9
Entrez Gene ID: 101085328
Clonality: Monoclonal
Immunogen: Denatured masseter myosin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: Myosin heavy chain, Type IIA, fast
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/26/2007
Antigen Molecular Weight: Predicted: 223 kDa
Depositor: Lucas, C.
Antigen Sequence:
Depositor Institution: University of Sydney
Antigen Species: cat
Depositor Notes: 2F7 reacts specifically with 2A myosin heavy chain from rat limb muscle by western blot, and with 2A muscle fibres on guinea pig, rat, mouse, rabbit and cat limb muscles by immunohistochemistry.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Baboon, Bandicoot, Canine, Cat, Cheetah, Guinea Pig, Human, Marsupial, Mouse, Opossum, Rabbit, Rat, Tiger
Additional Information: On western blots of dog myosin, 2F7 reacts with all myosin isoforms.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F7 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
Hoh JF
Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.