2F7

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SKU: 2F7-s

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Available: 99

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 2F7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MYH2
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_1157865 
Uniprot ID: M3WDH9 
RRID:  
Entrez Gene ID: 101085328 
Clonality: Monoclonal
Immunogen: Denatured masseter myosin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: Myosin heavy chain, Type IIA, fast
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/26/2007
Antigen Molecular Weight: Predicted: 223 kDa
Depositor: Lucas, C.
Antigen Sequence:
Depositor Institution: University of Sydney
Antigen Species: cat
Depositor Notes: 2F7 reacts specifically with 2A myosin heavy chain from rat limb muscle by western blot, and with 2A muscle fibres on guinea pig, rat, mouse, rabbit and cat limb muscles by immunohistochemistry.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Baboon, Bandicoot, Canine, Cat, Cheetah, Guinea Pig, Human, Marsupial, Mouse, Opossum, Rabbit, Rat, Tiger
Additional Information: On western blots of dog myosin, 2F7 reacts with all myosin isoforms.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F7 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    IF References
    WB References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums.
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 297.8 (2014 Aug): 1364-76.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle.
    Reiser PJ
    American journal of physiology. Cell physiology 290.5 (2006 May): C1446-58.

    IHC References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums.
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 297.8 (2014 Aug): 1364-76.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
    Guttridge DC
    The Journal of cell biology 196.4 (2012 Feb 20): 497-511.

    Fiber types in canine muscles: myosin isoform expression and functional characterization.
    Mascarello F
    American journal of physiology. Cell physiology 292.5 (2007 May): C1915-26.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    PHD3 Loss Promotes Exercise Capacity and Fat Oxidation in Skeletal Muscle.
    Haigis MC
    Cell metabolism 32.2 (2020 Aug 4): 215-228.e7.

    All References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums.
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 297.8 (2014 Aug): 1364-76.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
    Guttridge DC
    The Journal of cell biology 196.4 (2012 Feb 20): 497-511.

    Fiber types in canine muscles: myosin isoform expression and functional characterization.
    Mascarello F
    American journal of physiology. Cell physiology 292.5 (2007 May): C1915-26.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    PHD3 Loss Promotes Exercise Capacity and Fat Oxidation in Skeletal Muscle.
    Haigis MC
    Cell metabolism 32.2 (2020 Aug 4): 215-228.e7.

    Development transitions of thin filament proteins in rat extraocular muscles.
    Andrade FH
    Experimental cell research 319.3 (2013 Feb 1): 23-31.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles.
    Andrade FH
    Experimental cell research 316.12 (2010 Jul 15): 1958-65.

    Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice.
    Kuang S
    The Journal of biological chemistry 294.37 (2019 Sep 13): 13718-13728.

    Divergence exists in the subcellular distribution of intramuscular triglyceride in human skeletal muscle dependent on the choice of lipid dye.
    Shepherd SO
    Histochemistry and cell biology 154.4 (2020 Oct): 369-382.

    Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle.
    Reiser PJ
    American journal of physiology. Cell physiology 290.5 (2006 May): C1446-58.

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