2F7

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$40.00
SKU: 2F7
View product citations for antibody 2F7 on CiteAb

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Available: 120

DSHB Data Sheet

Catalog Fields

Antigen: myosin heavy chain, fast, 2A (MYH2)
Hybridoma Cells Available:
Antigen Species: cat
Depositor: Lucas, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Baboon, Bandicoot, Canine, Cat, Cheetah, Guinea Pig, Human, Marsupial, Mouse, Opossum, Rabbit, Rat, Tiger
Depositors Notes: 2F7 reacts specifically with 2A myosin heavy chain from rat limb muscle by western blot, and with 2A muscle fibres on guinea pig, rat, mouse, rabbit and cat limb muscles by immunohistochemistry.
Antigen Molecular Weight: Predicted: 223 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: Denatured masseter myosin
Alternate Gene Names: MyHC-2A
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: M3WDH9 
Immunogen Sequence: Full length protein
Entrez Gene ID: 101085328 
Additional Characterization:
Antibody Registry ID: AB_1161647 
Additional Information: On western blots of dog myosin, 2F7 reacts with all myosin isoforms.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F7 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    IF References
    WB References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums.
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 297.8 (2014 Aug): 1364-76.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle.
    Reiser PJ
    American journal of physiology. Cell physiology 290.5 (2006 May): C1446-58.

    IHC References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums.
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 297.8 (2014 Aug): 1364-76.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
    Guttridge DC
    The Journal of cell biology 196.4 (2012 Feb 20): 497-511.

    Fiber types in canine muscles: myosin isoform expression and functional characterization.
    Mascarello F
    American journal of physiology. Cell physiology 292.5 (2007 May): C1915-26.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    All References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Myosin isoform expression in the prehensile tails of didelphid marsupials: functional differences between arboreal and terrestrial opossums.
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 297.8 (2014 Aug): 1364-76.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.
    Guttridge DC
    The Journal of cell biology 196.4 (2012 Feb 20): 497-511.

    Fiber types in canine muscles: myosin isoform expression and functional characterization.
    Mascarello F
    American journal of physiology. Cell physiology 292.5 (2007 May): C1915-26.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    Development transitions of thin filament proteins in rat extraocular muscles.
    Andrade FH
    Experimental cell research 319.3 (2013 Feb 1): 23-31.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles.
    Andrade FH
    Experimental cell research 316.12 (2010 Jul 15): 1958-65.

    Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice.
    Kuang S
    The Journal of biological chemistry 294.37 (2019 Sep 13): 13718-13728.

    Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle.
    Reiser PJ
    American journal of physiology. Cell physiology 290.5 (2006 May): C1446-58.

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