Antigen: Myosin heavy chain, Type IIA, fast
Hybridoma Cells Available: No
Antigen Species: cat
Depositor: Lucas, C.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Baboon, Bandicoot, Canine, Cat, Cheetah, Guinea Pig, Human, Marsupial, Mouse, Opossum, Rabbit, Rat, Tiger
Depositors Notes: 2F7 reacts specifically with 2A myosin heavy chain from rat limb muscle by western blot, and with 2A muscle fibres on guinea pig, rat, mouse, rabbit and cat limb muscles by immunohistochemistry.
Predicted Species Reactivity:
Immunogen: Denatured masseter myosin
Alternate Gene Names: MyHC-2A
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: M3WDH9
Immunogen Sequence: Full length protein
Entrez Gene ID: 101085328
Antibody Registry ID: AB_1161647
Additional Information: On western blots of dog myosin, 2F7 reacts with all myosin isoforms.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F7 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.