2F7
Catalog Fields
Antigen: myosin heavy chain, fast, 2A (MYH2)
Depositor: Lucas, C.
Antigen Species: cat
Depositors Institution: University of Sydney
Isotype: MIgG1, kappa light chain
Depositors Notes: 2F7 reacts specifically with 2A myosin heavy chain from rat limb muscle by western blot, and with 2A muscle fibres on guinea pig, rat, mouse, rabbit and cat limb muscles by immunohistochemistry.
Host Species: mouse
Gene: MYH2
Positive Tested Species Reactivity: Baboon, Bandicoot, Canine, Cat, Cheetah, Guinea Pig, Human, Marsupial, Mouse, Opossum, Rabbit, Rat, Tiger
Alternate Gene Names: MyHC-2A
Antigen Molecular Weight: Predicted: 223 kDa
Clonality: Monoclonal
Immunogen: Denatured masseter myosin
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Additional Information: On western blots of dog myosin, 2F7 reacts with all myosin isoforms.
Uniprot ID: M3WDH9
Entrez Gene ID: 101085328
Antibody Registry ID: AB_1161647
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F7 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
Hoh JF
Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.