Antigen: Myosin Heavy Chain Type IIA
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Host Species: mouse
Depositors Institution: University of Padova
Positive Tested Species Reactivity: Bovine, Canine, Goat, Horse, Human, Mole, Mouse, Porcine, Rabbit, Rat, Sheep
Depositors Notes: This antibody recognizes the fast twitch isoform MyHC IIA specifically in horses and rodents. SC-71 does recognize both IIA and 2X in human and canine muscle (PMID: 18840559 and 22530000).
Antigen Molecular Weight: 223 kD Predicted
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Purified myosin isolated from subcutaneous muscle
Alternate Gene Names: MyHC-2a
Alternate Antibody Name: SC-71
Alternate Antigen Name: Myosin heavy chain 2A
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: Q9BE41
Immunogen Sequence: Full length protein
Entrez Gene ID: 788772
Antibody Registry ID: AB_2147165
Additional Information: This antibody has also tested positive on elephant seal, guinea pig, llama and goat muscle.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SC-71 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product SC-71)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).