SC-71
Catalog Fields
Clone ID/Product Name: SC-71
Available to For-Profits: Yes
Alternate Antibody Name: SC-71
Gene Symbol: MYH2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2147165
Uniprot ID: Q9BE41
Entrez Gene ID: 788772
Clonality: Monoclonal
Immunogen: Purified myosin isolated from subcutaneous muscle
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS0
Epitope Mapped: No
Antigen Name: Myosin heavy chain Type IIA
Epitope Location or Sequence:
Alternate Antigen Name: Myosin heavy chain 2A
Deposit Date: 3/25/2009
Antigen Molecular Weight: 223 kD Predicted
Depositor: Schiaffino, S.
Antigen Sequence:
Depositor Institution: Universita degli Studi di Padova
Antigen Species: Bovine
Depositor Notes: This antibody recognizes the fast twitch isoform MyHC IIA specifically in horses and rodents. SC-71 does recognize both IIA and 2X in human and canine muscle (PMID: 18840559 and 22530000).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Canine, Goat, Horse, Human, Mole, Mouse, Porcine, Rabbit, Rat, Sheep
Additional Information: This antibody has also tested positive on elephant seal, guinea pig, llama and goat muscle.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SC-71 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product SC-71)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
91 References
Initial Publication