10F5

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$40.00
SKU: 10F5
View product citations for antibody 10F5 on CiteAb

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Available: 107

DSHB Data Sheet

Catalog Fields

Antigen: myosin heavy chain, fast, 2B
Hybridoma Cells Available:
Antigen Species: Rabbit
Depositor: Lucas, C.
Isotype: MIgM, kappa light chain
Antigen Sequence: myosin
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Antechinus, Bandicoot, Bettongia, Cheetah, Guinea Pig, Human, Kangaroo, Mouse, Porcine, Pseudocheirus, Rabbit, Rat, Trichosurus, Wallaby
Depositors Notes:
Antigen Molecular Weight: Predicted: 223kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH4
Immunogen: rabbit retractor bulbi muscle
Alternate Gene Names: MYH1, MHC
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: Q28641 
Immunogen Sequence:
Entrez Gene ID: 100125991 
Additional Characterization:
Antibody Registry ID: AB_1157896 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10F5 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 10F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

27 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    IF References

    Proteomics analysis of the cardiac myofilament subproteome reveals dynamic alterations in phosphatase subunit distribution.
    Mayr M
    Molecular and cellular proteomics : MCP 9.3 (2010 Mar): 497-509.

    Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles.
    Andrade FH
    Experimental cell research 316.12 (2010 Jul 15): 1958-65.

    Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity.
    Kershaw EE
    Diabetes 62.10 (2013 Oct): 3350-61.

    Muscle-specific AMPK β1β2-null mice display a myopathy due to loss of capillary density in nonpostural muscles.
    Hawke TJ
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28.5 (2014 May): 2098-107.

    AAV-mediated overexpression of human α7 integrin leads to histological and functional improvement in dystrophic mice.
    Rodino-Klapac LR
    Molecular therapy : the journal of the American Society of Gene Therapy 21.3 (2013 Mar): 520-5.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue.
    Muscat GE
    Molecular endocrinology (Baltimore, Md.) 26.3 (2012 Mar): 372-84.

    WB References
    IHC References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles.
    Erzen I
    Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 247.11 (2009 Nov): 1505-15.

    Myosin heavy chain isoform expression in human extraocular muscles: longitudinal variation and patterns of expression in global and orbital layers.
    Oh SY
    Muscle and nerve 45.5 (2012 May): 713-20.

    Early activation of rat skeletal muscle IL-6/STAT1/STAT3 dependent gene expression in resistance exercise linked to hypertrophy.
    Py G
    PloS one 8.2 (2013): e57141.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    Relationship between pork quality and characteristics of muscle fibers classified by the distribution of myosin heavy chain isoforms.
    Joo ST
    Journal of animal science 91.11 (2013 Nov): 5525-34.

    Muscle ciliary neurotrophic factor receptor α promotes axonal regeneration and functional recovery following peripheral nerve lesion.
    Maclennan AJ
    The Journal of comparative neurology 521.13 (2013 Sep 1): 2947-65.

    Electrophoretic and immunochemical evidence showing that marsupial limb muscles express the same fast and slow myosin heavy chains as eutherians.
    Hoh JF
    Electrophoresis 22.6 (2001 Apr): 1016-20.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    Calpastatin overexpression in the skeletal muscle of mice prevents clenbuterol-induced muscle hypertrophy and phenotypic shift.
    Py G
    Clinical and experimental pharmacology and physiology 39.4 (2012 Apr): 364-72.

    Immunohistochemical analysis of the effects of cross-innervation of murine thyroarytenoid and sternohyoid muscles.
    Hoh JF
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 58.12 (2010 Dec): 1057-65.

    Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, Antechinus flavipes.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 180.4 (2010 Apr): 531-44.

    All References

    Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
    Hoh JF
    Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.

    Histochemical and immunohistochemical profile of human and rat ocular medial rectus muscles.
    Erzen I
    Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 247.11 (2009 Nov): 1505-15.

    Myosin heavy chain isoform expression in human extraocular muscles: longitudinal variation and patterns of expression in global and orbital layers.
    Oh SY
    Muscle and nerve 45.5 (2012 May): 713-20.

    Early activation of rat skeletal muscle IL-6/STAT1/STAT3 dependent gene expression in resistance exercise linked to hypertrophy.
    Py G
    PloS one 8.2 (2013): e57141.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    Relationship between pork quality and characteristics of muscle fibers classified by the distribution of myosin heavy chain isoforms.
    Joo ST
    Journal of animal science 91.11 (2013 Nov): 5525-34.

    Muscle ciliary neurotrophic factor receptor α promotes axonal regeneration and functional recovery following peripheral nerve lesion.
    Maclennan AJ
    The Journal of comparative neurology 521.13 (2013 Sep 1): 2947-65.

    Electrophoretic and immunochemical evidence showing that marsupial limb muscles express the same fast and slow myosin heavy chains as eutherians.
    Hoh JF
    Electrophoresis 22.6 (2001 Apr): 1016-20.

    Myosin isoforms and fibre types in limb muscles of Australian marsupials: adaptations to hopping and non-hopping locomotion.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 47-55.

    Calpastatin overexpression in the skeletal muscle of mice prevents clenbuterol-induced muscle hypertrophy and phenotypic shift.
    Py G
    Clinical and experimental pharmacology and physiology 39.4 (2012 Apr): 364-72.

    Immunohistochemical analysis of the effects of cross-innervation of murine thyroarytenoid and sternohyoid muscles.
    Hoh JF
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 58.12 (2010 Dec): 1057-65.

    Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, Antechinus flavipes.
    Hoh JF
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 180.4 (2010 Apr): 531-44.

    Proteomics analysis of the cardiac myofilament subproteome reveals dynamic alterations in phosphatase subunit distribution.
    Mayr M
    Molecular and cellular proteomics : MCP 9.3 (2010 Mar): 497-509.

    Nonmuscle myosin IIB, a sarcomeric component in the extraocular muscles.
    Andrade FH
    Experimental cell research 316.12 (2010 Jul 15): 1958-65.

    Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity.
    Kershaw EE
    Diabetes 62.10 (2013 Oct): 3350-61.

    Muscle-specific AMPK β1β2-null mice display a myopathy due to loss of capillary density in nonpostural muscles.
    Hawke TJ
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28.5 (2014 May): 2098-107.

    AAV-mediated overexpression of human α7 integrin leads to histological and functional improvement in dystrophic mice.
    Rodino-Klapac LR
    Molecular therapy : the journal of the American Society of Gene Therapy 21.3 (2013 Mar): 520-5.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue.
    Muscat GE
    Molecular endocrinology (Baltimore, Md.) 26.3 (2012 Mar): 372-84.

    Differential expression of myosin heavy chain isoforms between abductor and adductor muscles in the human larynx.
    Flint PW
    Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 130.2 (2004 Feb): 217-22.

    Identification of myosin heavy chain isoforms in porcine longissimus dorsi muscle by electrophoresis and mass spectrometry.
    Joo ST
    Electrophoresis 34.9-10 (2013 May): 1255-61.

    Myosin heavy chain expression pattern as a marker for anabolic potency: desoxymethyltestosterone (madol), norandrostenedione and testosterone repress MHC-IIb expression and stimulate MHC-IId/x expression in orchiectomized rat gastrocnemius muscle.
    Diel P
    Archives of toxicology 85.6 (2011 Jun): 635-43.

    Myosin isoform fiber type and fiber size in the tail of the Virginia opossum (Didelphis virginiana).
    Butcher MT
    Anatomical record (Hoboken, N.J. : 2007) 296.1 (2013 Jan): 96-107.

    Fiber type and metabolic characteristics of lion (Panthera leo), caracal (Caracal caracal) and human skeletal muscle.
    Noakes TD
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 159.2 (2011 Jun): 125-33.

    Immunohistochemical analysis of laryngeal muscles in normal horses and horses with subclinical recurrent laryngeal neuropathy.
    Hoh JF
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.8 (2009 Aug): 787-800.

    High oxidative capacity and type IIx fibre content in springbok and fallow deer skeletal muscle suggest fast sprinters with a resistance to fatigue.
    Kohn TA
    The Journal of experimental biology 215.Pt 22 (2012 Nov 15): 3997-4005.

    Cross-bridge kinetics of fast and slow fibres of cat jaw and limb muscles: correlations with myosin subunit composition.
    Rossmanith GH
    Journal of muscle research and cell motility 28.6 (2007): 329-41.

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