BF-35

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$40.00
SKU: BF-35
View product citations for antibody BF-35 on CiteAb

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Available: 107

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (all but 2X)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Padova
Positive Tested Species Reactivity: Bovine, Canine, Equine, Feline, Goat, Guinea Pig, Human, Mole, Porcine, Rodent
Depositors Notes: Fusion: 1986. Recognizes type 1, 2A, and 2B fibers in skeletal muscle.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: purified myosin from bovine skeletal muscle (fetal, 3 months)
Alternate Gene Names: MyHC-2a
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: Q9BE41 
Immunogen Sequence:
Entrez Gene ID: 788772 
Additional Characterization:
Antibody Registry ID: AB_2274680 
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-35 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    IF References
    WB References

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes.
    Rivero JL
    Cell and tissue research 323.2 (2006 Feb): 283-303.

    IHC References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study.
    Edgerton VR
    The Anatomical record 246.2 (1996 Oct): 185-94.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    Cellular adaptations in the diaphragm in chronic obstructive pulmonary disease.
    Tikunov B
    The New England journal of medicine 337.25 (1997 Dec 18): 1799-806.

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes.
    Rivero JL
    Cell and tissue research 323.2 (2006 Feb): 283-303.

    Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Proportions of myosin heavy chain mRNAs, protein isoforms and fiber types in the slow and fast skeletal muscles are maintained after alterations of thyroid status in rats.
    Diallo M
    Physiological research 64.1 (2015): 111-8.

    Multiparametric functional nuclear magnetic resonance imaging shows alterations associated with plasmid electrotransfer in mouse skeletal muscle.
    Carlier PG
    The journal of gene medicine 14.9-10 (2012 Sep-Oct): 598-608.

    Amyloid precursor protein (APP) contributes to pathology in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis.
    Greensmith L
    Human molecular genetics 21.17 (2012 Sep 1): 3871-82.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Sternohyoid and diaphragm muscle form and function during postnatal development in the rat.
    O'Halloran KD
    Experimental physiology 98.9 (2013 Sep): 1386-400.

    Characteristics of Muscle Fiber-Type Distribution in Moles.
    Wada N
    Anatomical record (Hoboken, N.J. : 2007) 302.6 (2019 Jun): 1010-1023.

    All References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study.
    Edgerton VR
    The Anatomical record 246.2 (1996 Oct): 185-94.

    Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.
    Talmadge RJ
    Journal of experimental zoology. Part A, Ecological genetics and physiology 313.1 (2010 Jan 1): 45-57.

    Cellular adaptations in the diaphragm in chronic obstructive pulmonary disease.
    Tikunov B
    The New England journal of medicine 337.25 (1997 Dec 18): 1799-806.

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes.
    Rivero JL
    Cell and tissue research 323.2 (2006 Feb): 283-303.

    Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Proportions of myosin heavy chain mRNAs, protein isoforms and fiber types in the slow and fast skeletal muscles are maintained after alterations of thyroid status in rats.
    Diallo M
    Physiological research 64.1 (2015): 111-8.

    Multiparametric functional nuclear magnetic resonance imaging shows alterations associated with plasmid electrotransfer in mouse skeletal muscle.
    Carlier PG
    The journal of gene medicine 14.9-10 (2012 Sep-Oct): 598-608.

    Amyloid precursor protein (APP) contributes to pathology in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis.
    Greensmith L
    Human molecular genetics 21.17 (2012 Sep 1): 3871-82.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Sternohyoid and diaphragm muscle form and function during postnatal development in the rat.
    O'Halloran KD
    Experimental physiology 98.9 (2013 Sep): 1386-400.

    Characteristics of Muscle Fiber-Type Distribution in Moles.
    Wada N
    Anatomical record (Hoboken, N.J. : 2007) 302.6 (2019 Jun): 1010-1023.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle.
    Kaminski HJ
    The Journal of physiology 589.Pt 18 (2011 Sep 15): 4601-14.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology and visual science 51.12 (2010 Dec): 6355-63.

    Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
    Quadrilatero J
    PloS one 7.4 (2012): e35273.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

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