BF-35
Catalog Fields
Antigen: Myosin heavy chain (all but Type IIX)
Available to For-Profits: Yes
Antigen Species: Bovine
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Schiaffino, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Bovine, Canine, Equine, Feline, Goat, Guinea Pig, Human, Mole, Porcine, Rodent
Depositors Institution: Universita degli Studi di Padova
Antigen Molecular Weight: 220kDa
Depositors Notes: Fusion: 1986. Recognizes type 1, 2A, and 2B fibers in skeletal muscle.
Immunogen: purified myosin from bovine skeletal muscle (fetal, 3 months)
Gene: MYH2
Alternate Antibody Name:
Alternate Gene Names: MyHC-2a
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS0
Epitope Mapped: No
Uniprot ID: Q9BE41
Epitope Location or Sequence:
Entrez Gene ID: 788772
Immunogen Sequence:
Antibody Registry ID: AB_2274680
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-35 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
20 References
Initial Publication
Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
Lømo T
Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.