5e

Catalog Fields
Clone ID/Product Name: 5e
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: NCAM1
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2058005
Uniprot ID: P13590
Entrez Gene ID: 428253
Clonality: Monoclonal
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3-X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: NCAM (extracellular domain), all isoforms
Epitope Location or Sequence: The epitope is a polypeptide determinant located on the extracellular region of NCAM (25 kDa subtilisin heparin-binding fragment); estimated to be located around 150 aa from N-terminus.
Alternate Antigen Name:
Deposit Date: 4/25/1990
Antigen Molecular Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Depositor: Rutishauser, U.
Antigen Sequence:
Depositor Institution: Memorial Sloan-Kettering Cancer Center
Antigen Species: chicken
Depositor Notes: Doesn't cross react with frog (Rana pipiens) NCAM. Attenuates NCAM-NCAM binding. Satins all NCAM positive tissues.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken
Additional Information: Recognizes all isoforms. 5e has been reported to work on Bufo arenarum [PMID: 8901203] but doesn't appear to work on rana pipiens [PMID: 2307708]
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

