5e

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$40.00
SKU: 5e
View product citations for antibody 5e on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: NCAM (extracellular domain), all isoforms
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Rutishauser, U.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Memorial Sloan-Kettering Cancer Center
Positive Tested Species Reactivity: Chicken
Depositors Notes: Doesn't cross react with frog (Rana pipiens) NCAM. Attenuates NCAM-NCAM binding. Satins all NCAM positive tissues.
Antigen Molecular Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: NCAM1
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Alternate Gene Names: NACM, N-CAM
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653
Epitope Location or Sequence: The epitope is a polypeptide determinant located on the extracellular region of NCAM (25 kDa subtilisin heparin-binding fragment); estimated to be located around 150 aa from N-terminus.
Uniprot ID: P13590 
Immunogen Sequence: Full length protein
Entrez Gene ID: 428253 
Additional Characterization:  
Antibody Registry ID: AB_2058005 
Additional Information: Recognizes all isoforms. 5e has been reported to work on Bufo arenarum [PMID: 8901203] but doesn't appear to work on rana pipiens [PMID: 2307708]
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5e was deposited to the DSHB by Rutishauser, U. (DSHB Hybridoma Product 5e)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1721-7.

    Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
    Landmesser L
    The Journal of cell biology 132.5 (1996 Mar): 969-83.

    Expression of isoforms of the neural cell adhesion molecule (NCAM) and polysialic acid during the development of the Bufo arenarum olfactory system.
    Peralta Soler A
    The International journal of developmental biology 39.6 (1995 Dec): 1005-13.

    Effects of polysialic acid on sensory innervation of the cornea.
    Conrad GW
    Developmental biology 398.2 (2015 Feb 15): 193-205.

    Polysialylated NCAM expression on endocardial cells of the chick primary atrial septum.
    Watanabe M
    The Anatomical record 247.1 (1997 Jan): 71-84.

    Regulation and activity-dependence of N-cadherin, NCAM isoforms, and polysialic acid on chick myotubes during development.
    Landmesser L
    The Journal of cell biology 123.6 Pt 2 (1993 Dec): 1867-88.

    N-CAM and N-cadherin expression during in vitro chondrogenesis.
    Castagnola P
    Experimental cell research 215.2 (1994 Dec): 354-62.

    Neural differentiation, NCAM-mediated adhesion, and gap junctional communication in neuroectoderm. A study in vitro.
    Rutishauser U
    The Journal of cell biology 106.4 (1988 Apr): 1307-19.

    Comparison of neurite outgrowth induced by intact and injured sciatic nerves: a confocal and functional analysis.
    Cochard P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 328-38.

    WB References
    IHC References

    Polysialylated NCAM expression on endocardial cells of the chick primary atrial septum.
    Watanabe M
    The Anatomical record 247.1 (1997 Jan): 71-84.

    Regulation and activity-dependence of N-cadherin, NCAM isoforms, and polysialic acid on chick myotubes during development.
    Landmesser L
    The Journal of cell biology 123.6 Pt 2 (1993 Dec): 1867-88.

    Comparison of neurite outgrowth induced by intact and injured sciatic nerves: a confocal and functional analysis.
    Cochard P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 328-38.

    IP References
    ELISA References
    FB References
    Epitope Map References
    All References

    Polysialylated NCAM expression on endocardial cells of the chick primary atrial septum.
    Watanabe M
    The Anatomical record 247.1 (1997 Jan): 71-84.

    Regulation and activity-dependence of N-cadherin, NCAM isoforms, and polysialic acid on chick myotubes during development.
    Landmesser L
    The Journal of cell biology 123.6 Pt 2 (1993 Dec): 1867-88.

    Comparison of neurite outgrowth induced by intact and injured sciatic nerves: a confocal and functional analysis.
    Cochard P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 328-38.

    Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1721-7.

    Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
    Landmesser L
    The Journal of cell biology 132.5 (1996 Mar): 969-83.

    Expression of isoforms of the neural cell adhesion molecule (NCAM) and polysialic acid during the development of the Bufo arenarum olfactory system.
    Peralta Soler A
    The International journal of developmental biology 39.6 (1995 Dec): 1005-13.

    Effects of polysialic acid on sensory innervation of the cornea.
    Conrad GW
    Developmental biology 398.2 (2015 Feb 15): 193-205.

    N-CAM and N-cadherin expression during in vitro chondrogenesis.
    Castagnola P
    Experimental cell research 215.2 (1994 Dec): 354-62.

    Neural differentiation, NCAM-mediated adhesion, and gap junctional communication in neuroectoderm. A study in vitro.
    Rutishauser U
    The Journal of cell biology 106.4 (1988 Apr): 1307-19.

    Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1729-37.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina.
    Godbout R
    FEBS letters 588.5 (2014 Mar 3): 770-5.

    Regulation of neural cell adhesion molecule polysialylation: evidence for nontranscriptional control and sensitivity to an intracellular pool of calcium.
    Rutishauser U
    The Journal of cell biology 140.5 (1998 Mar 9): 1177-86.

    NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
    Rutishauser U
    The Journal of cell biology 114.1 (1991 Jul): 143-53.

    A bird's eye view of enteric nervous system development: lessons from the avian embryo.
    Nagy N
    Pediatric research 64.4 (2008 Oct): 326-33.

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