5e
Catalog Fields
Antigen: NCAM (extracellular domain), all isoforms
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Rutishauser, U.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Memorial Sloan-Kettering Cancer Center
Positive Tested Species Reactivity: Chicken
Depositors Notes: Doesn't cross react with frog (Rana pipiens) NCAM. Attenuates NCAM-NCAM binding. Satins all NCAM positive tissues.
Antigen Molecular Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Predicted Species Reactivity:
Gene: NCAM1
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Alternate Gene Names: NACM, N-CAM
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653
Epitope Location or Sequence: The epitope is a polypeptide determinant located on the extracellular region of NCAM (25 kDa subtilisin heparin-binding fragment); estimated to be located around 150 aa from N-terminus.
Uniprot ID: P13590
Immunogen Sequence: Full length protein
Entrez Gene ID: 428253
Antibody Registry ID: AB_2058005
Additional Information: Recognizes all isoforms. 5e has been reported to work on Bufo arenarum [PMID: 8901203] but doesn't appear to work on rana pipiens [PMID: 2307708]
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5e was deposited to the DSHB by Rutishauser, U. (DSHB Hybridoma Product 5e)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
Rutishauser U
The Journal of cell biology 103.5 (1986 Nov): 1721-7.