Clone ID / Name: 5e
Anti - NCAM (extracellular domain), all isoforms
Catalog Fields
Identity
Clone ID / Name: 5e
Alternate Antibody Name: —
Antigen Name: NCAM (extracellular domain), all isoforms
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: NCAM1
Entrez Gene ID: 428253
Antigen Sequence: —
Antigen Species: chicken
Antigen Mol. Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Uniprot ID: P13590
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: P3-X63-Ag8.653
Antibody Registry / RRID: AB_2058005
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: The epitope is a polypeptide determinant located on the extracellular region of NCAM (25 kDa subtilisin heparin-binding fragment); estimated to be located around 150 aa from N-terminus.
Clone: —
Additional Info: Recognizes all isoforms. 5e has been reported to work on Bufo arenarum [PMID: 8901203] but doesn't appear to work on rana pipiens [PMID: 2307708]
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Chicken
Predicted Reactivity: —
Depositor
Depositor Name: U. Rutishauser
Institution: Memorial Sloan-Kettering Cancer Center
Deposit Date: April 25, 1990
Depositor Notes: Doesn't cross react with frog (Rana pipiens) NCAM. Attenuates NCAM-NCAM binding. Satins all NCAM positive tissues.
Recommended Applications
ELISA
Function Blocking
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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