4d

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$40.00
SKU: 4d
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DSHB Data Sheet

Catalog Fields

Antigen: NCAM (cytoplasmic domain), 180 kDa isoform
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Rutishauser, U.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Memorial Sloan-Kettering Cancer Center
Positive Tested Species Reactivity: Avian, Chicken, Frog, Xenopus
Depositors Notes: Stains all neurons with axons
Antigen Molecular Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NCAM1
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Alternate Gene Names: NCAM, N-CAM
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653
Epitope Location or Sequence: The epitope is a polypeptide determinant located on the cytoplasmic region of NCAM ( estimated to be located around 180 aa from C-terminus)
Uniprot ID: P13590 
Immunogen Sequence: Full length protein
Entrez Gene ID: 428253 
Additional Characterization:
Antibody Registry ID: AB_528389 
Additional Information: Recognizes only the longest isoform (180 kDa)
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4d was deposited to the DSHB by Rutishauser, U. (DSHB Hybridoma Product 4d)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1721-7.

    Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1729-37.

    Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
    Landmesser L
    The Journal of cell biology 132.5 (1996 Mar): 969-83.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    Changes in neural cell adhesion molecule (NCAM) structure during vertebrate neural development.
    Jacobson M
    Proceedings of the National Academy of Sciences of the United States of America 84.16 (1987 Aug): 5986-90.

    NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain.
    Hoffman S
    The Journal of cell biology 128.1-2 (1995 Jan): 209-21.

    Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos.
    Rutishauser U
    Developmental biology 116.2 (1986 Aug): 524-31.

    IHC References

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole.
    Mochii M
    BMC developmental biology 14. (2014 Jun 18): 27.

    Anatomical specializations for enhanced olfactory sensitivity in kiwi, Apteryx mantelli.
    Parsons S
    Brain, behavior and evolution 84.3 (2014): 214-26.

    Vertical versus planar neural induction in Rana pipiens embryos.
    Dawid IB
    Proceedings of the National Academy of Sciences of the United States of America 91.8 (1994 Apr 12): 3049-53.

    Testing the effects of FSHD candidate gene expression in vertebrate muscle development.
    Jones PL
    International journal of clinical and experimental pathology 3.4 (2010 Mar 28): 386-400.

    Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis.
    Frontera JL
    Developmental neurobiology 77.11 (2017 Nov): 1308-1320.

    IP References

    Heterophilic NCAM-mediated cell adhesion to proteoglycans from chick embryonic brain membranes.
    Murray BA
    Experimental cell research 223.2 (1996 Mar 15): 385-94.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    ELISA References
    Epitope Map References
    All References

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole.
    Mochii M
    BMC developmental biology 14. (2014 Jun 18): 27.

    Anatomical specializations for enhanced olfactory sensitivity in kiwi, Apteryx mantelli.
    Parsons S
    Brain, behavior and evolution 84.3 (2014): 214-26.

    Vertical versus planar neural induction in Rana pipiens embryos.
    Dawid IB
    Proceedings of the National Academy of Sciences of the United States of America 91.8 (1994 Apr 12): 3049-53.

    Testing the effects of FSHD candidate gene expression in vertebrate muscle development.
    Jones PL
    International journal of clinical and experimental pathology 3.4 (2010 Mar 28): 386-400.

    Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis.
    Frontera JL
    Developmental neurobiology 77.11 (2017 Nov): 1308-1320.

    Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1721-7.

    Non-viral expression of mouse Oct4, Sox2, and Klf4 transcription factors efficiently reprograms tadpole muscle fibers in vivo.
    Coen L
    The Journal of biological chemistry 287.10 (2012 Mar 2): 7427-35.

    Expression of isoforms of the neural cell adhesion molecule (NCAM) and polysialic acid during the development of the Bufo arenarum olfactory system.
    Peralta Soler A
    The International journal of developmental biology 39.6 (1995 Dec): 1005-13.

    Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1729-37.

    Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
    Landmesser L
    The Journal of cell biology 132.5 (1996 Mar): 969-83.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    Changes in neural cell adhesion molecule (NCAM) structure during vertebrate neural development.
    Jacobson M
    Proceedings of the National Academy of Sciences of the United States of America 84.16 (1987 Aug): 5986-90.

    NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain.
    Hoffman S
    The Journal of cell biology 128.1-2 (1995 Jan): 209-21.

    Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos.
    Rutishauser U
    Developmental biology 116.2 (1986 Aug): 524-31.

    Heterophilic NCAM-mediated cell adhesion to proteoglycans from chick embryonic brain membranes.
    Murray BA
    Experimental cell research 223.2 (1996 Mar 15): 385-94.

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