4d
Catalog Fields
Antigen: NCAM (cytoplasmic domain), 180 kDa isoform
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Rutishauser, U.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Memorial Sloan-Kettering Cancer Center
Positive Tested Species Reactivity: Avian, Chicken, Frog, Xenopus
Depositors Notes: Stains all neurons with axons
Antigen Molecular Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Human Protein Atlas:
Predicted Species Reactivity:
Gene: NCAM1
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Alternate Gene Names: NCAM, N-CAM
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8.653
Epitope Location or Sequence: The epitope is a polypeptide determinant located on the cytoplasmic region of NCAM ( estimated to be located around 180 aa from C-terminus)
Uniprot ID: P13590
Immunogen Sequence: Full length protein
Entrez Gene ID: 428253
Additional Characterization:
Antibody Registry ID: AB_528389
Additional Information: Recognizes only the longest isoform (180 kDa)
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4d was deposited to the DSHB by Rutishauser, U. (DSHB Hybridoma Product 4d)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
16 References
Initial Publication
Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
Rutishauser U
The Journal of cell biology 103.5 (1986 Nov): 1721-7.