4d

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 4d
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: NCAM1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528389 
Uniprot ID: P13590 
RRID:  
Entrez Gene ID: 428253 
Clonality: Monoclonal
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3-X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: NCAM (cytoplasmic domain), 180 kDa isoform
Epitope Location or Sequence: The epitope is a polypeptide determinant located on the cytoplasmic region of NCAM ( estimated to be located around 180 aa from C-terminus)
Alternate Antigen Name:
Deposit Date: 4/25/1990
Antigen Molecular Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Depositor: Rutishauser, U.
Antigen Sequence:
Depositor Institution: Memorial Sloan-Kettering Cancer Center
Antigen Species: chicken
Depositor Notes: Stains all neurons with axons
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Chicken, Frog, Xenopus
Additional Information: Recognizes only the longest isoform (180 kDa)
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4d was deposited to the DSHB by Rutishauser, U. (DSHB Hybridoma Product 4d)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1721-7.

    Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1729-37.

    Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
    Landmesser L
    The Journal of cell biology 132.5 (1996 Mar): 969-83.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    Changes in neural cell adhesion molecule (NCAM) structure during vertebrate neural development.
    Jacobson M
    Proceedings of the National Academy of Sciences of the United States of America 84.16 (1987 Aug): 5986-90.

    NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain.
    Hoffman S
    The Journal of cell biology 128.1-2 (1995 Jan): 209-21.

    Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos.
    Rutishauser U
    Developmental biology 116.2 (1986 Aug): 524-31.

    IHC References

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole.
    Mochii M
    BMC developmental biology 14. (2014 Jun 18): 27.

    Anatomical specializations for enhanced olfactory sensitivity in kiwi, Apteryx mantelli.
    Parsons S
    Brain, behavior and evolution 84.3 (2014): 214-26.

    Vertical versus planar neural induction in Rana pipiens embryos.
    Dawid IB
    Proceedings of the National Academy of Sciences of the United States of America 91.8 (1994 Apr 12): 3049-53.

    Testing the effects of FSHD candidate gene expression in vertebrate muscle development.
    Jones PL
    International journal of clinical and experimental pathology 3.4 (2010 Mar 28): 386-400.

    Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis.
    Frontera JL
    Developmental neurobiology 77.11 (2017 Nov): 1308-1320.

    IP References

    Heterophilic NCAM-mediated cell adhesion to proteoglycans from chick embryonic brain membranes.
    Murray BA
    Experimental cell research 223.2 (1996 Mar 15): 385-94.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    ELISA References
    Epitope Map References
    All References

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole.
    Mochii M
    BMC developmental biology 14. (2014 Jun 18): 27.

    Anatomical specializations for enhanced olfactory sensitivity in kiwi, Apteryx mantelli.
    Parsons S
    Brain, behavior and evolution 84.3 (2014): 214-26.

    Vertical versus planar neural induction in Rana pipiens embryos.
    Dawid IB
    Proceedings of the National Academy of Sciences of the United States of America 91.8 (1994 Apr 12): 3049-53.

    Testing the effects of FSHD candidate gene expression in vertebrate muscle development.
    Jones PL
    International journal of clinical and experimental pathology 3.4 (2010 Mar 28): 386-400.

    Neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of Xenopus laevis.
    Frontera JL
    Developmental neurobiology 77.11 (2017 Nov): 1308-1320.

    Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1721-7.

    Non-viral expression of mouse Oct4, Sox2, and Klf4 transcription factors efficiently reprograms tadpole muscle fibers in vivo.
    Coen L
    The Journal of biological chemistry 287.10 (2012 Mar 2): 7427-35.

    Expression of isoforms of the neural cell adhesion molecule (NCAM) and polysialic acid during the development of the Bufo arenarum olfactory system.
    Peralta Soler A
    The International journal of developmental biology 39.6 (1995 Dec): 1005-13.

    Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
    Rutishauser U
    The Journal of cell biology 103.5 (1986 Nov): 1729-37.

    Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
    Landmesser L
    The Journal of cell biology 132.5 (1996 Mar): 969-83.

    Binding properties of detergent-solubilized NCAM.
    Rutishauser U
    The Journal of cell biology 110.3 (1990 Mar): 817-24.

    Changes in neural cell adhesion molecule (NCAM) structure during vertebrate neural development.
    Jacobson M
    Proceedings of the National Academy of Sciences of the United States of America 84.16 (1987 Aug): 5986-90.

    NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain.
    Hoffman S
    The Journal of cell biology 128.1-2 (1995 Jan): 209-21.

    Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos.
    Rutishauser U
    Developmental biology 116.2 (1986 Aug): 524-31.

    Heterophilic NCAM-mediated cell adhesion to proteoglycans from chick embryonic brain membranes.
    Murray BA
    Experimental cell research 223.2 (1996 Mar 15): 385-94.

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