Antibody Name: 4d
Antigen:NCAM (cytoplasmic domain), 180 kDa isoform
Immunofluorescent image of a stage 40 Xenopus embryo labeled with DSHB antibody 4d against NCAM. Image Credit: Annette Borchers, PMID: 32424618, Fig. 5E.
Catalog Fields
Identity
Clone ID / Antibody Name: 4d
Alternate Antibody Name: —
Antigen Name: NCAM (cytoplasmic domain), 180 kDa isoform
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: NCAM1
Entrez Gene ID: 428253
Antigen Sequence: —
Antigen Species: chicken
Antigen Mol. Weight: Predicted: multiple isoforms of 78, 90, 111, and 115 kDa; Apparent: 120, 140, 1nd 180 kDa.
Uniprot ID: P13590
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: P3-X63-Ag8.653
Antibody Registry / RRID: AB_528389
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Purified NCAM from embryonic chicken brain membranes (E14)
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: The epitope is a polypeptide determinant located on the cytoplasmic region of NCAM ( estimated to be located around 180 aa from C-terminus)
Clone: —
Additional Info: Recognizes only the longest isoform (180 kDa)
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Avian, Chicken, Frog, Xenopus
Predicted Reactivity: —
Depositor
Depositor Name: U. Rutishauser
Institution: Memorial Sloan-Kettering Cancer Center
Deposit Date: April 25, 1990
Depositor Notes: Stains all neurons with axons
Recommended Applications
ELISA
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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