CYP33E1

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$40.00
SKU: CYP33E1
View product citations for antibody CYP33E1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CYP-33E1 (CYtochrome P450 family)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts. Stains the endoplasmic reticulum. Works poorly on immunoblots.
Antigen Molecular Weight: Predicted; 58 kDa; Apparent: 73 kDa (with two faint bands of 90 and 110 kDa)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: cyp-33e1
Immunogen: Recombinant His6-tagged CYP-33E1 fusion protein
Alternate Gene Names:
Alternate Antibody Name: 1G3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q27482 
Immunogen Sequence: full length protein
Entrez Gene ID: 183602 
Additional Characterization:
Antibody Registry ID: AB_10571938 
Additional Information: The CYtochrome P450 family protein is a cytochrome P450 family member involved in the metabolism of isoniazid and likely localized to the endoplasmic reticulum similarly to most P450 family members.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CYP33E1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CYP33E1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

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