Catalog Fields
Antigen: pancreatic duodenal homeobox protein 1 (Pdx1)
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Madsen, O.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: Pdx1 is a homeobox transcription factor required for pancreas formation. F6A11 doesn't recognize human Pdx1.
Antigen Molecular Weight: Predicted: 31kDa; Apparent: 39 kDa and additional band at 70 kDa
Predicted Species Reactivity:
Gene: Pdx1
Immunogen: Recombinant GST/Pdx1 (aa 215-283) fusion protein
Alternate Gene Names: Ipf1, Idx1, Stf1
Alternate Antibody Name: F109
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: C-terminal aa 215-283.
Uniprot ID: P52947
Immunogen Sequence: Partial protein
Entrez Gene ID: 29535
Antibody Registry ID: AB_1157903
Additional Information:
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F109-D12 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F109-D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
Hald J
European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.