F109-D12
Catalog Fields
Antigen: pancreatic duodenal homeobox protein 1 (Pdx1)
Hybridoma Cells Available:
Antigen Species: Rat
Depositor: Madsen, O.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hagedorn Research Institute, Denmark
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: Pdx1 is a homeobox transcription factor required for pancreas formation. F6A11 doesn't recognize human Pdx1.
Antigen Molecular Weight: Predicted: 31kDa; Apparent: 39 kDa and additional band at 70 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Pdx1
Immunogen: Recombinant GST/Pdx1 (aa 215-283) fusion protein
Alternate Gene Names: Ipf1, Idx1, Stf1
Alternate Antibody Name: F109
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: C-terminal aa 215-283.
Uniprot ID: P52947
Immunogen Sequence: Partial protein
Entrez Gene ID: 29535
Additional Characterization:
Antibody Registry ID: AB_1157903
Additional Information:
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F109-D12 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F109-D12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
Hald J
European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.