F55A12

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SKU: F55A12

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DSHB Data Sheet

Catalog Fields

Product Name/ID: F55A12
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Nkx6-1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_532379 
Uniprot ID: O35762 
RRID:  
Entrez Gene ID: 65193 
Clonality: Monoclonal
Immunogen: GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Clone:
Immunogen Sequence: a.a. 299-365
Myeloma Strain: Fox-NY
Epitope Mapped: Yes
Antigen Name: Homeobox protein Nkx-6.1; Nkx6.1
Epitope Location or Sequence: C-terminal a.a. 299-365, DDDYNKP
Alternate Antigen Name:
Deposit Date: 3/15/2006
Antigen Molecular Weight: 44-46 kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: Rat
Depositor Notes: This antibody is also available from the Beta Cell Consortium at http://www.betacell.org/ab/ab_view.php?AB_ID=2023
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bovine, Human, Mouse, Porcine, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, FFPE, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F55A12 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F55A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    IF References

    Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Bang AG
    Nature biotechnology 29.8 (2011 Jul 31): 750-6.

    Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Inverardi L
    Cell transplantation 21.6 (2012): 1321-39.

    PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
    Liu A
    Developmental dynamics : an official publication of the American Association of Anatomists 240.8 (2011 Aug): 1938-48.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Bang AG
    Nature biotechnology 29.8 (2011 Jul 31): 750-6.

    Controlling fluid flow to improve cell seeding uniformity.
    Gadegaard N
    PloS one 13.11 (2018): e0207211.

    Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.
    Ardestani A
    Cell reports 36.5 (2021 Aug 3): 109490.

    WB References

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    IHC References
    FACS References
    Epitope Map References

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    All References

    Immunohistochemistry of pancreatic development in cattle and pig.
    Hyttel P
    Anatomia, histologia, embryologia 39.2 (2010 Apr): 107-19.

    PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
    Liu A
    Developmental dynamics : an official publication of the American Association of Anatomists 240.8 (2011 Aug): 1938-48.

    Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells.
    Zhou Q
    Nature biotechnology 32.12 (2014 Dec): 1223-30.

    Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions.
    Laurent LC
    Journal of cell science 126.Pt 17 (2013 Sep 1): 3848-61.

    Highly Proliferative α-Cell-Related Islet Endocrine Cells in Human Pancreata.
    Kushner JA
    Diabetes 67.4 (2018 Apr): 674-686.

    A Conserved Notochord Enhancer Controls Pancreas Development in Vertebrates.
    Bessa J
    Cell reports 32.1 (2020 Jul 7): 107862.

    Efficient induction of pancreatic alpha cells from human induced pluripotent stem cells by controlling the timing for BMP antagonism and activation of retinoic acid signaling.
    Okochi H
    PloS one 16.1 (2021): e0245204.

    Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Bang AG
    Nature biotechnology 29.8 (2011 Jul 31): 750-6.

    Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Inverardi L
    Cell transplantation 21.6 (2012): 1321-39.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Controlling fluid flow to improve cell seeding uniformity.
    Gadegaard N
    PloS one 13.11 (2018): e0207211.

    Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.
    Ardestani A
    Cell reports 36.5 (2021 Aug 3): 109490.

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

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