Antigen: Homeobox protein Nkx-6.1; Nkx6.1
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Madsen, O.D.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Bovine, Human, Mouse, Porcine, Rat
Depositors Notes: This antibody is also available from the Beta Cell Consortium at http://www.betacell.org/ab/ab_view.php?AB_ID=2023
Predicted Species Reactivity:
Immunogen: GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Alternate Gene Names: Nkx61; Nkx6a; Nkx6.1
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: C-terminal a.a. 299-365, DDDYNKP
Uniprot ID: O35762
Immunogen Sequence: a.a. 299-365
Entrez Gene ID: 65193
Antibody Registry ID: AB_532379
Recommended Applications: FACS, FFPE, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F55A12 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F55A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
Epitope Map References