F55A12

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$40.00
SKU: F55A12
View product citations for antibody F55A12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Homeobox protein Nkx-6.1; Nkx6.1
Hybridoma Cells Available:
Antigen Species: Rat
Depositor: Madsen, O.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Bovine, Human, Mouse, Porcine, Rat
Depositors Notes: Link 
Antigen Molecular Weight: 44-46 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Nkx6-1
Immunogen: GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Alternate Gene Names: Nkx61; Nkx6a; Nkx6.1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: C-terminal a.a. 299-365, DDDYNKP
Uniprot ID: O35762 
Immunogen Sequence: a.a. 299-365
Entrez Gene ID: 65193 
Additional Characterization:
Antibody Registry ID: AB_532379 
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F55A12 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F55A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    IF References

    Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Bang AG
    Nature biotechnology 29.8 (2011 Jul 31): 750-6.

    Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Inverardi L
    Cell transplantation 21.6 (2012): 1321-39.

    PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
    Liu A
    Developmental dynamics : an official publication of the American Association of Anatomists 240.8 (2011 Aug): 1938-48.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Bang AG
    Nature biotechnology 29.8 (2011 Jul 31): 750-6.

    Controlling fluid flow to improve cell seeding uniformity.
    Gadegaard N
    PloS one 13.11 (2018): e0207211.

    WB References

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    IHC References

    Immunohistochemistry of pancreatic development in cattle and pig.
    Hyttel P
    Anatomia, histologia, embryologia 39.2 (2010 Apr): 107-19.

    PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
    Liu A
    Developmental dynamics : an official publication of the American Association of Anatomists 240.8 (2011 Aug): 1938-48.

    Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells.
    Zhou Q
    Nature biotechnology 32.12 (2014 Dec): 1223-30.

    Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions.
    Laurent LC
    Journal of cell science 126.Pt 17 (2013 Sep 1): 3848-61.

    FACS References
    Epitope Map References

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    All References

    Immunohistochemistry of pancreatic development in cattle and pig.
    Hyttel P
    Anatomia, histologia, embryologia 39.2 (2010 Apr): 107-19.

    PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
    Liu A
    Developmental dynamics : an official publication of the American Association of Anatomists 240.8 (2011 Aug): 1938-48.

    Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells.
    Zhou Q
    Nature biotechnology 32.12 (2014 Dec): 1223-30.

    Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions.
    Laurent LC
    Journal of cell science 126.Pt 17 (2013 Sep 1): 3848-61.

    Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Bang AG
    Nature biotechnology 29.8 (2011 Jul 31): 750-6.

    Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Inverardi L
    Cell transplantation 21.6 (2012): 1321-39.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Controlling fluid flow to improve cell seeding uniformity.
    Gadegaard N
    PloS one 13.11 (2018): e0207211.

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

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