40.2D6

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$40.00
SKU: 40.2D6
View product citations for antibody 40.2D6 on CiteAb

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Available: 158

DSHB Data Sheet

Catalog Fields

Antigen: Islet-1 homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: altricial fish larva, Chicken, Fish, Frog, Human, Mouse, Newt, Planaria, Porcine, Rat, Reptile, Vertebrates, Xenopus, Zebrafish
Depositors Notes: Reacted with all tested vertebrate species. The 40.2D6 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.3A4 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Isl1
Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .
Alternate Gene Names: Isl-1; isl-1=homeobox
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P61374 
Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)
Entrez Gene ID: 64444 
Additional Characterization:
Antibody Registry ID: AB_528315 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FACS, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40.2D6 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.2D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

72 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FACS References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    IF References

    Diabetes
    Ducy P
    Diabetes 63.3 (2014 Mar): 1021-31.

    PloS one
    Gorivodsky M
    PloS one 9.4 (2014): e95256.

    PloS one
    Butchbach ME
    PloS one 9.9 (2014): e106818.

    Developmental biology
    Taneyhill LA
    Developmental biology 392.2 (2014 Aug 15): 295-307.

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    BMC biology
    Kawakami K
    BMC biology 12. (2014 May 29): 40.

    Developmental neurobiology
    James J
    Developmental neurobiology 74.12 (2014 Dec): 1155-71.

    Nature communications
    Swaroop A
    Nature communications 5. (2014 Jun 20): 4207.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Marunouchi T
    Developmental dynamics : an official publication of the American Association of Anatomists 243.4 (2014 Apr): 588-600.

    Development (Cambridge, England)
    Robert B
    Development (Cambridge, England) 141.8 (2014 Apr): 1726-36.

    Glia
    Legendre P
    Glia 59.4 (2011 Apr): 675-95.

    The Journal of comparative neurology
    Kullander K
    The Journal of comparative neurology 518.12 (2010 Jun 15): 2284-304.

    Development (Cambridge, England)
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Mechanisms of development
    Ang SL
    Mechanisms of development 128.1-2 (2011 Jan-Feb): 90-103.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Johnson JE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.30 (2011 Jul 27): 10859-71.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.13 (2012 Mar 28): 4426-39.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Bach I
    Developmental dynamics : an official publication of the American Association of Anatomists 235.3 (2006 Mar): 786-91.

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Nature
    Svendsen CN
    Nature 457.7227 (2009 Jan 15): 277-80.

    Mechanisms of development
    Bach I
    Mechanisms of development 117.1-2 (2002 Sep): 75-85.

    Neuron
    Greenberg ME
    Neuron 58.1 (2008 Apr 10): 65-77.

    Developmental biology
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    deLapeyrière O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4992-5000.

    Genesis (New York, N.Y. : 2000)
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Developmental biology
    Camper SA
    Developmental biology 304.2 (2007 Apr 15): 455-66.

    Developmental biology
    Camper SA
    Developmental biology 305.1 (2007 May 1): 145-60.

    Developmental biology
    Camper SA
    Developmental biology 313.1 (2008 Jan 1): 118-29.

    The Journal of comparative neurology
    Sherwood P
    The Journal of comparative neurology 506.4 (2008 Feb 1): 584-603.

    Nature cell biology
    Harris WA
    Nature cell biology 14.8 (2012 Aug): 859-64.

    The Journal of comparative neurology
    Price DJ
    The Journal of comparative neurology 512.5 (2009 Feb 10): 613-27.

    Neurobiology of disease
    Suzuki N
    Neurobiology of disease 20.1 (2005 Oct): 38-48.

    Nature biotechnology
    Eggan K
    Nature biotechnology 29.3 (2011 Mar): 279-86.

    Nature biotechnology
    Studer L
    Nature biotechnology 30.7 (2012 Jul 1): 715-20.

    Cell transplantation
    West FD
    Cell transplantation 22.6 (2013): 945-59.

    The Journal of comparative neurology
    González A
    The Journal of comparative neurology 521.1 (2013 Jan 1): 24-49.

    The Journal of comparative neurology
    Yaginuma H
    The Journal of comparative neurology 521.13 (2013 Sep 1): 2987-3002.

    Development (Cambridge, England)
    Nakagawa S
    Development (Cambridge, England) 130.3 (2003 Feb): 587-98.

    Neuroscience research
    Shiga T
    Neuroscience research 52.1 (2005 May): 11-9.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Fischbach GD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.4 (1997 Feb 15): 1416-24.

    Neuroscience
    Fischer AJ
    Neuroscience 169.3 (2010 Sep 1): 1376-91.

    Proceedings of the National Academy of Sciences of the United States of America
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 96.20 (1999 Sep 28): 10992-9.

    Experimental eye research
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Journal of experimental zoology. Part B, Molecular and developmental evolution
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    WB References

    BMC biology
    Cui S
    BMC biology 12. (2014 Mar 27): 25.

    The Journal of biological chemistry
    Reguenga C
    The Journal of biological chemistry 288.51 (2013 Dec 20): 36285-301.

    Developmental biology
    Morrison JI
    Developmental biology 383.2 (2013 Nov 15): 253-63.

    The Journal of comparative neurology
    González A
    The Journal of comparative neurology 521.1 (2013 Jan 1): 24-49.

    IHC References

    BMC biology
    Cui S
    BMC biology 12. (2014 Mar 27): 25.

    Journal of neuropathology and experimental neurology
    Golden JA
    Journal of neuropathology and experimental neurology 73.3 (2014 Mar): 253-61.

    PloS one
    Gorivodsky M
    PloS one 9.4 (2014): e95256.

    American journal of physiology. Gastrointestinal and liver physiology
    May CL
    American journal of physiology. Gastrointestinal and liver physiology 307.10 (2014 Nov 15): G979-91.

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    BMC biology
    Kawakami K
    BMC biology 12. (2014 May 29): 40.

    Developmental biology
    Ang SL
    Developmental biology 333.2 (2009 Sep 15): 386-96.

    Mechanisms of development
    Bach I
    Mechanisms of development 117.1-2 (2002 Sep): 75-85.

    Neuron
    Okamoto H
    Neuron 30.2 (2001 May): 423-36.

    Developmental biology
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    Pigment cell research
    Domínguez L
    Pigment cell research 18.4 (2005 Aug): 243-51.

    Brain research
    Martín-Partido G
    Brain research 1103.1 (2006 Aug 4): 32-48.

    Neurobiology of disease
    Suzuki N
    Neurobiology of disease 20.1 (2005 Oct): 38-48.

    International journal of cardiology
    Meeson AP
    International journal of cardiology 156.2 (2012 Apr 19): 133-8.

    Neural development
    Pratt T
    Neural development 4. (2009 Jan 16): 3.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Becker T
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.34 (2008 Aug 20): 8510-6.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.18 (2007 May 2): 4978-83.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Higashijima S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.21 (2006 May 24): 5684-97.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Becker CG
    Developmental dynamics : an official publication of the American Association of Anatomists 234.3 (2005 Nov): 535-49.

    Development (Cambridge, England)
    Eisen JS
    Development (Cambridge, England) 131.4 (2004 Feb): 891-902.

    Experimental eye research
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Journal of experimental zoology. Part B, Molecular and developmental evolution
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Nature communications
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    FFPE References

    Diabetes
    Ducy P
    Diabetes 63.3 (2014 Mar): 1021-31.

    BMC biology
    Cui S
    BMC biology 12. (2014 Mar 27): 25.

    PloS one
    Gorivodsky M
    PloS one 9.4 (2014): e95256.

    American journal of physiology. Gastrointestinal and liver physiology
    May CL
    American journal of physiology. Gastrointestinal and liver physiology 307.10 (2014 Nov 15): G979-91.

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    Development (Cambridge, England)
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Brain research
    Martín-Partido G
    Brain research 1103.1 (2006 Aug 4): 32-48.

    Developmental biology
    Camper SA
    Developmental biology 304.2 (2007 Apr 15): 455-66.

    FACS References

    Nature cell biology
    Harris WA
    Nature cell biology 14.8 (2012 Aug): 859-64.

    Epitope Map References

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Neuron
    Edlund T
    Neuron 7.6 (1991 Dec): 881-9.

    Gel Supershift References

    BMC biology
    Cui S
    BMC biology 12. (2014 Mar 27): 25.

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    The Journal of biological chemistry
    May CL
    The Journal of biological chemistry 286.17 (2011 Apr 29): 15352-60.

    All References

    BMC biology
    Cui S
    BMC biology 12. (2014 Mar 27): 25.

    Journal of neuropathology and experimental neurology
    Golden JA
    Journal of neuropathology and experimental neurology 73.3 (2014 Mar): 253-61.

    PloS one
    Gorivodsky M
    PloS one 9.4 (2014): e95256.

    American journal of physiology. Gastrointestinal and liver physiology
    May CL
    American journal of physiology. Gastrointestinal and liver physiology 307.10 (2014 Nov 15): G979-91.

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    BMC biology
    Kawakami K
    BMC biology 12. (2014 May 29): 40.

    Developmental biology
    Ang SL
    Developmental biology 333.2 (2009 Sep 15): 386-96.

    Mechanisms of development
    Bach I
    Mechanisms of development 117.1-2 (2002 Sep): 75-85.

    Neuron
    Okamoto H
    Neuron 30.2 (2001 May): 423-36.

    Developmental biology
    Henderson CE
    Developmental biology 214.1 (1999 Oct 1): 60-71.

    Pigment cell research
    Domínguez L
    Pigment cell research 18.4 (2005 Aug): 243-51.

    Brain research
    Martín-Partido G
    Brain research 1103.1 (2006 Aug 4): 32-48.

    Neurobiology of disease
    Suzuki N
    Neurobiology of disease 20.1 (2005 Oct): 38-48.

    International journal of cardiology
    Meeson AP
    International journal of cardiology 156.2 (2012 Apr 19): 133-8.

    Neural development
    Pratt T
    Neural development 4. (2009 Jan 16): 3.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Becker T
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.34 (2008 Aug 20): 8510-6.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.18 (2007 May 2): 4978-83.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Higashijima S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.21 (2006 May 24): 5684-97.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Becker CG
    Developmental dynamics : an official publication of the American Association of Anatomists 234.3 (2005 Nov): 535-49.

    Development (Cambridge, England)
    Eisen JS
    Development (Cambridge, England) 131.4 (2004 Feb): 891-902.

    Experimental eye research
    Francisco-Morcillo J
    Experimental eye research 89.3 (2009 Sep): 398-415.

    Journal of experimental zoology. Part B, Molecular and developmental evolution
    Francisco-Morcillo J
    Journal of experimental zoology. Part B, Molecular and developmental evolution 314.7 (2010 Nov 15): 580-605.

    Nature communications
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    Diabetes
    Ducy P
    Diabetes 63.3 (2014 Mar): 1021-31.

    PloS one
    Butchbach ME
    PloS one 9.9 (2014): e106818.

    Developmental biology
    Taneyhill LA
    Developmental biology 392.2 (2014 Aug 15): 295-307.

    Developmental neurobiology
    James J
    Developmental neurobiology 74.12 (2014 Dec): 1155-71.

    Nature communications
    Swaroop A
    Nature communications 5. (2014 Jun 20): 4207.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Marunouchi T
    Developmental dynamics : an official publication of the American Association of Anatomists 243.4 (2014 Apr): 588-600.

    Development (Cambridge, England)
    Robert B
    Development (Cambridge, England) 141.8 (2014 Apr): 1726-36.

    Glia
    Legendre P
    Glia 59.4 (2011 Apr): 675-95.

    The Journal of comparative neurology
    Kullander K
    The Journal of comparative neurology 518.12 (2010 Jun 15): 2284-304.

    Development (Cambridge, England)
    Rane SG
    Development (Cambridge, England) 138.10 (2011 May): 1903-12.

    Mechanisms of development
    Ang SL
    Mechanisms of development 128.1-2 (2011 Jan-Feb): 90-103.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Johnson JE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.30 (2011 Jul 27): 10859-71.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.13 (2012 Mar 28): 4426-39.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Bach I
    Developmental dynamics : an official publication of the American Association of Anatomists 235.3 (2006 Mar): 786-91.

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Nature
    Svendsen CN
    Nature 457.7227 (2009 Jan 15): 277-80.

    Neuron
    Greenberg ME
    Neuron 58.1 (2008 Apr 10): 65-77.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    deLapeyrière O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.13 (2000 Jul 1): 4992-5000.

    Genesis (New York, N.Y. : 2000)
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Developmental biology
    Camper SA
    Developmental biology 304.2 (2007 Apr 15): 455-66.

    Developmental biology
    Camper SA
    Developmental biology 305.1 (2007 May 1): 145-60.

    Developmental biology
    Camper SA
    Developmental biology 313.1 (2008 Jan 1): 118-29.

    The Journal of comparative neurology
    Sherwood P
    The Journal of comparative neurology 506.4 (2008 Feb 1): 584-603.

    Nature cell biology
    Harris WA
    Nature cell biology 14.8 (2012 Aug): 859-64.

    The Journal of comparative neurology
    Price DJ
    The Journal of comparative neurology 512.5 (2009 Feb 10): 613-27.

    Nature biotechnology
    Eggan K
    Nature biotechnology 29.3 (2011 Mar): 279-86.

    Nature biotechnology
    Studer L
    Nature biotechnology 30.7 (2012 Jul 1): 715-20.

    Cell transplantation
    West FD
    Cell transplantation 22.6 (2013): 945-59.

    The Journal of comparative neurology
    González A
    The Journal of comparative neurology 521.1 (2013 Jan 1): 24-49.

    The Journal of comparative neurology
    Yaginuma H
    The Journal of comparative neurology 521.13 (2013 Sep 1): 2987-3002.

    Development (Cambridge, England)
    Nakagawa S
    Development (Cambridge, England) 130.3 (2003 Feb): 587-98.

    Neuroscience research
    Shiga T
    Neuroscience research 52.1 (2005 May): 11-9.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Fischbach GD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.4 (1997 Feb 15): 1416-24.

    Neuroscience
    Fischer AJ
    Neuroscience 169.3 (2010 Sep 1): 1376-91.

    Proceedings of the National Academy of Sciences of the United States of America
    Beachy PA
    Proceedings of the National Academy of Sciences of the United States of America 96.20 (1999 Sep 28): 10992-9.

    The Journal of biological chemistry
    Reguenga C
    The Journal of biological chemistry 288.51 (2013 Dec 20): 36285-301.

    Developmental biology
    Morrison JI
    Developmental biology 383.2 (2013 Nov 15): 253-63.

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Neuron
    Edlund T
    Neuron 7.6 (1991 Dec): 881-9.

    The Journal of biological chemistry
    May CL
    The Journal of biological chemistry 286.17 (2011 Apr 29): 15352-60.

    Endocrinology
    Cui S
    Endocrinology 151.10 (2010 Oct): 4787-800.

    PloS one
    Gross MK
    PloS one 7.12 (2012): e48573.

    Neuron
    Henderson CE
    Neuron 17.1 (1996 Jul): 63-74.

    Cell
    Tessier-Lavigne M
    Cell 87.6 (1996 Dec 13): 1001-14.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Ito K
    Developmental dynamics : an official publication of the American Association of Anatomists 214.1 (1999 Jan): 55-65.

    Journal of neuroscience research
    deLapeyrière O
    Journal of neuroscience research 55.1 (1999 Jan 1): 119-26.

    Methods in molecular biology (Clifton, N.J.)
    Ziegler L
    Methods in molecular biology (Clifton, N.J.) 584. (2010): 283-300.

    Methods in molecular biology (Clifton, N.J.)
    Goldstein RS
    Methods in molecular biology (Clifton, N.J.) 584. (2010): 367-85.

    Proceedings of the National Academy of Sciences of the United States of America
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

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