F55A10

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$40.00
SKU: F55A10
View product citations for antibody F55A10 on CiteAb

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Available: 365

DSHB Data Sheet

Catalog Fields

Antigen: Homeobox protein Nkx-6.1; Nkx6.1
Hybridoma Cells Available:
Antigen Species: Rat
Depositor: Madsen, O.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Amphibian, Chicken, Fish, Human, Mouse, Porcine, Rat, Zebrafish
Depositors Notes: This antibody does not cross react with Nkx6.2. F55A10 competes for binding with F55A12, but can bind simultaneously with F64A6B4 or F65A2.
Antigen Molecular Weight: 44-46 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Nkx6-1
Immunogen: GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Alternate Gene Names: Nkx61; Nkx6a; Nkx6.1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Fox-NY
Epitope Location or Sequence: C-terminal a.a. 299-365, DDDYNKP
Uniprot ID: O35762 
Immunogen Sequence: a.a. 299-365
Entrez Gene ID: 65193 
Additional Characterization:
Antibody Registry ID: AB_532378 
Additional Information:
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F55A10 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F55A10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

36 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    IF References

    Sonic hedgehog is a polarized signal for motor neuron regeneration in adult zebrafish.
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.48 (2009 Dec 2): 15073-82.

    Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Lectin-based isolation and culture of mouse embryonic motoneurons.
    Klausmeyer A
    Journal of visualized experiments : JoVE .55 (2011 Sep 15): .

    The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling.
    Jackson PK
    Cell 152.1-2 (2013 Jan 17): 210-23.

    Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
    Lowry WE
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 806-11.

    An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs.
    Hecksher-Sørensen J
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.9 (2007 Sep): 925-30.

    Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system.
    Kim J
    Proceedings of the National Academy of Sciences of the United States of America 111.7 (2014 Feb 18): 2794-9.

    Establishment of motor neuron-V3 interneuron progenitor domain boundary in ventral spinal cord requires Groucho-mediated transcriptional corepression.
    Stifani S
    PloS one 7.2 (2012): e31176.

    Lectin-based isolation and culture of mouse embryonic motoneurons.
    Klausmeyer A
    Journal of visualized experiments : JoVE .55 (2011 Sep 15): .

    Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration.
    Stainier DY
    Developmental biology 334.1 (2009 Oct 1): 213-23.

    IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes.
    Herman AE
    PloS one 8.10 (2013): e78483.

    Pdx-1 activates islet α- and β-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2.
    Hohmeier HE
    Molecular and cellular biology 33.20 (2013 Oct): 4017-29.

    Lesion-induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish.
    Becker CG
    The Journal of comparative neurology 520.16 (2012 Nov 1): 3604-16.

    Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development.
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro.
    Bauchet L
    Journal of neuroscience research 86.9 (2008 Jul): 1916-26.

    Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
    Rice CM
    Cell 172.3 (2018 Jan 25): 423-438.e25.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    WB References

    Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord.
    Hugnot JP
    BMC neuroscience 12. (2011 Oct 10): 99.

    Spatiotemporal patterns of Pax3, Pax6, and Pax7 expression in the developing brain of a urodele amphibian, Pleurodeles waltl.
    Moreno N
    The Journal of comparative neurology 521.17 (2013 Dec 1): 3913-53.

    Pdx-1 activates islet α- and β-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2.
    Hohmeier HE
    Molecular and cellular biology 33.20 (2013 Oct): 4017-29.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    IHC References

    IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes.
    Herman AE
    PloS one 8.10 (2013): e78483.

    Wnt5a cooperates with canonical Wnts to generate midbrain dopaminergic neurons in vivo and in stem cells.
    Arenas E
    Proceedings of the National Academy of Sciences of the United States of America 110.7 (2013 Feb 12): E602-10.

    Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration.
    Peers B
    Developmental biology 366.2 (2012 Jun 15): 268-78.

    Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons.
    Partanen J
    Developmental biology 343.1-2 (2010 Jul 1): 63-70.

    Sonic hedgehog is a polarized signal for motor neuron regeneration in adult zebrafish.
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.48 (2009 Dec 2): 15073-82.

    Tulp3 is a critical repressor of mouse hedgehog signaling.
    Petkovich M
    Developmental dynamics : an official publication of the American Association of Anatomists 238.5 (2009 May): 1140-9.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
    Lowry WE
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 806-11.

    An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs.
    Hecksher-Sørensen J
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.9 (2007 Sep): 925-30.

    Lesion-induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish.
    Becker CG
    The Journal of comparative neurology 520.16 (2012 Nov 1): 3604-16.

    Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube.
    Anderson KV
    Development (Cambridge, England) 138.22 (2011 Nov): 4921-30.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

    Spatiotemporal patterns of Pax3, Pax6, and Pax7 expression in the developing brain of a urodele amphibian, Pleurodeles waltl.
    Moreno N
    The Journal of comparative neurology 521.17 (2013 Dec 1): 3913-53.

    Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel-Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and Wnt signalling defects.
    Johnson CA
    Human molecular genetics 22.7 (2013 Apr 1): 1358-72.

    Spatiotemporally restricted regulation of generic motor neuron programs by miR-196-mediated repression of Hoxb8.
    Kessel M
    Developmental biology 344.2 (2010 Aug 15): 857-68.

    Gata2 is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons.
    Partanen J
    Development (Cambridge, England) 136.2 (2009 Jan): 253-62.

    Analysis of hedgehog signaling in mouse intraflagellar transport mutants.
    Eggenschwiler JT
    Methods in cell biology 93. (2009): 347-69.

    Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel-Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and Wnt signalling defects.
    Johnson CA
    Human molecular genetics 22.7 (2013 Apr 1): 1358-72.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

    Immunohistochemistry of pancreatic development in cattle and pig.
    Hyttel P
    Anatomia, histologia, embryologia 39.2 (2010 Apr): 107-19.

    Cell Mass Increase Associated with Formation of Glucose-Controlling β-Cell Mass in Device-Encapsulated Implants of hiPS-Derived Pancreatic Endoderm.
    Pipeleers DG
    Stem cells translational medicine 8.12 (2019 Dec): 1296-1305.

    FFPE References
    FACS References
    Epitope Map References

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    All References

    IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes.
    Herman AE
    PloS one 8.10 (2013): e78483.

    Wnt5a cooperates with canonical Wnts to generate midbrain dopaminergic neurons in vivo and in stem cells.
    Arenas E
    Proceedings of the National Academy of Sciences of the United States of America 110.7 (2013 Feb 12): E602-10.

    Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration.
    Peers B
    Developmental biology 366.2 (2012 Jun 15): 268-78.

    Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons.
    Partanen J
    Developmental biology 343.1-2 (2010 Jul 1): 63-70.

    Sonic hedgehog is a polarized signal for motor neuron regeneration in adult zebrafish.
    Becker CG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.48 (2009 Dec 2): 15073-82.

    Tulp3 is a critical repressor of mouse hedgehog signaling.
    Petkovich M
    Developmental dynamics : an official publication of the American Association of Anatomists 238.5 (2009 May): 1140-9.

    Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
    Jorgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.

    Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
    Lowry WE
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 806-11.

    An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs.
    Hecksher-Sørensen J
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55.9 (2007 Sep): 925-30.

    Lesion-induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish.
    Becker CG
    The Journal of comparative neurology 520.16 (2012 Nov 1): 3604-16.

    Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube.
    Anderson KV
    Development (Cambridge, England) 138.22 (2011 Nov): 4921-30.

    Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling.
    Novitch BG
    Development (Cambridge, England) 138.15 (2011 Aug): 3199-211.

    Spatiotemporal patterns of Pax3, Pax6, and Pax7 expression in the developing brain of a urodele amphibian, Pleurodeles waltl.
    Moreno N
    The Journal of comparative neurology 521.17 (2013 Dec 1): 3913-53.

    Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel-Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and Wnt signalling defects.
    Johnson CA
    Human molecular genetics 22.7 (2013 Apr 1): 1358-72.

    Spatiotemporally restricted regulation of generic motor neuron programs by miR-196-mediated repression of Hoxb8.
    Kessel M
    Developmental biology 344.2 (2010 Aug 15): 857-68.

    Gata2 is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons.
    Partanen J
    Development (Cambridge, England) 136.2 (2009 Jan): 253-62.

    Analysis of hedgehog signaling in mouse intraflagellar transport mutants.
    Eggenschwiler JT
    Methods in cell biology 93. (2009): 347-69.

    Immunohistochemistry of pancreatic development in cattle and pig.
    Hyttel P
    Anatomia, histologia, embryologia 39.2 (2010 Apr): 107-19.

    Cell Mass Increase Associated with Formation of Glucose-Controlling β-Cell Mass in Device-Encapsulated Implants of hiPS-Derived Pancreatic Endoderm.
    Pipeleers DG
    Stem cells translational medicine 8.12 (2019 Dec): 1296-1305.

    Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
    Clotman F
    Neuroscience 165.1 (2010 Jan 13): 116-29.

    Lectin-based isolation and culture of mouse embryonic motoneurons.
    Klausmeyer A
    Journal of visualized experiments : JoVE .55 (2011 Sep 15): .

    The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling.
    Jackson PK
    Cell 152.1-2 (2013 Jan 17): 210-23.

    Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system.
    Kim J
    Proceedings of the National Academy of Sciences of the United States of America 111.7 (2014 Feb 18): 2794-9.

    Establishment of motor neuron-V3 interneuron progenitor domain boundary in ventral spinal cord requires Groucho-mediated transcriptional corepression.
    Stifani S
    PloS one 7.2 (2012): e31176.

    Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration.
    Stainier DY
    Developmental biology 334.1 (2009 Oct 1): 213-23.

    Pdx-1 activates islet α- and β-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2.
    Hohmeier HE
    Molecular and cellular biology 33.20 (2013 Oct): 4017-29.

    Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development.
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro.
    Bauchet L
    Journal of neuroscience research 86.9 (2008 Jul): 1916-26.

    Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
    Rice CM
    Cell 172.3 (2018 Jan 25): 423-438.e25.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord.
    Hugnot JP
    BMC neuroscience 12. (2011 Oct 10): 99.

    Specificity of four monoclonal anti-NKx6-1 antibodies.
    Jørgensen MC
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 56.4 (2008 Apr): 415-24.

    Mycobacteria, cytokines and antibiotics.
    Rook GA
    Pathologie-biologie 38.4 (1990 Apr): 276-80.

    Modeling Monogenic Diabetes using Human ESCs Reveals Developmental and Metabolic Deficiencies Caused by Mutations in HNF1A.
    Gadue P
    Cell stem cell 25.2 (2019 Aug 1): 273-289.e5.

    Genetic dissection of Gata2 selective functions during specification of V2 interneurons in the developing spinal cord.
    Nardelli J
    Developmental neurobiology 75.7 (2015 Jul): 721-37.

    [The possibility of subcutaneous rupture of the Achilles tendon under the effect of a vertical tensile force (experimental study)].
    Karasev VI
    Ortopediia travmatologiia i protezirovanie 30.12 (1969 Dec): 41-3.

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